Genomic data reveals the emergence of the co-occurrence of blaKPC-2 and blaCTX-M-15 in an Escherichia coli ST648 strain isolated from rectal swab within the framework of hospital surveillance

Piekar, María; Álvarez, Verónica Elizabeth; Knecht, Camila A.; Leguina, Ana Carolina del Valle; Allende, Natalia; Páez, Laura Carrera; Gambino, Anahí S.; Machuca, Adrián González; Campos, Josefina; Fox, Barbara; Carpio, Eduardo; Aguilar, Andrea; Alonso, Fernando Martín; Canigia, Liliana Fernández; Quiroga, María Paula; Centrón, Daniela

doi:10.1016/j.jgar.2022.12.012

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Interestingly, two of these strains (E. coli ECO 37 isolated in 2014 and E. coli ECO 81 isolated in 2015) were isolated before the first description of the mcr-1 gene in isolates from animals, food, and patients in China (Liu et al, 2016). Escherichia coli ST615 was not identified in those epidemiological studies and in other studies performed with E. coli strains from Argentina isolated from the clinic or other environments before or after the COVID-19 pandemic (Sennati et al, 2012;Dominguez et al, 2019;Faccone et al, 2023;Gramundi et al, 2023;Piekar et al, 2023), confirming the sporadic condition of this clone. Escherichia coli M19736 ST615 strain isolated in 2015 was identified at that time as one of the first isolates harboring the mcr-1 gene in human infections caused by E. coli in Latin America (Rapoport et al, 2016).…”

Section: Discussionmentioning

confidence: 72%

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Carrera Páez,

Olivier,

Gambino

et al. 2024

Front. Cell. Infect. Microbiol.

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There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical Escherichia coli M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an mcr-1 gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids that harbor blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, or aadB genes. Escherichia coli M19736 acquired blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, and aadB genes, being only blaNDM-1 maintained at 100% on the 10th day of subculture. In addition, when the evolved MDR-E. coli M19736 acquired sequentially blaCTX-M-15 and blaNDM-1 genes, the maintenance pattern of the plasmids changed. In addition, when the evolved XDR-E. coli M19736 acquired in an ulterior step the paadB plasmid, a different pattern of the plasmid’s maintenance was found. Interestingly, the evolved E. coli M19736 strains disseminated simultaneously the acquired conjugative plasmids in different combinations though selection was ceftazidime in all cases. Finally, we isolated and characterized the extracellular vesicles (EVs) from the native and evolved XDR-E. coli M19736 strains. Interestingly, EVs from the evolved XDR-E. coli M19736 harbored blaCTX-M-15 though the pDCAG1-CTX-M-15 was previously lost as shown by WGS and experiments, suggesting that EV could be a relevant reservoir of ARG for susceptible bacteria. These results evidenced the genetic plasticity of a sporadic clone of E. coli such as ST615 that could play a relevant transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug-resistant phenotypes of superbugs within the nosocomial niche by acting simultaneously as a vector and reservoir of multiple ARGs which later could be disseminated.

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Section: Discussionmentioning

confidence: 72%

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Carrera Páez,

Olivier,

Gambino

et al. 2024

Front. Cell. Infect. Microbiol.

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“…Analysis of the genetic context of the bla KPC-2 gene revealed that it was located in the Tn4401a transposon, a member of the Tn3 transposon family ( 28 , 29 ). To our knowledge, this is one of the few reports on Tn4401a in E. coli ( 30 ). Of note, current data differ from the previous genetic environment of bla KPC-2 detected in Peru, highlighting the presence of different genetic structures carrying this gene in the country ( 7 ).…”

Section: Discussionmentioning

confidence: 84%

Molecular characterization of carbapenemase-producing Enterobacterales in a tertiary hospital in Lima, Peru

Cuicapuza,

Loyola,

Velásquez

et al. 2024

Microbiol Spectr

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Carbapenemase-producing Enterobacterales (CPE) are a growing threat to global health and the economy. Understanding the interactions between resistance and virulence mechanisms of CPE is crucial for managing difficult-to-treat infections and informing outbreak prevention and control programs. Here, we report the characterization of 21 consecutive, unique clinical isolates of CPE collected in 2018 at a tertiary hospital in Lima, Peru. Isolates were characterized by phenotypic antimicrobial susceptibility testing and whole-genome sequencing to identify resistance determinants and virulence factors. Seven Klebsiella pneumoniae isolates were classified as extensively drug-resistant. The remaining Klebsiella , Enterobacter hormaechei, and Escherichia coli isolates were multidrug-resistant. Eighteen strains carried the metallo-β-lactamase NDM-1, two the serine-carbapenemase KPC-2, and one isolate had both carbapenemases. The bla NDM-1 gene was located in the truncated ΔISAba125 element, and the bla KPC-2 gene was in the Tn4401a transposon. ST147 was the most frequent sequence type among K. pneumoniae isolates. Our findings highlight the urgent need to address the emergence of CPE and strengthen control measures and antibiotic stewardship programs in low- and middle-income settings. IMPORTANCE Genomic surveillance of antimicrobial resistance contributes to monitoring the spread of resistance and informs treatment and prevention strategies. We characterized 21 carbapenemase-producing Enterobacterales collected at a Peruvian tertiary hospital in 2018, which exhibited very high levels of resistance and carried numerous resistance genes. We detected the coexistence of carbapenemase-encoding genes ( bla NDM-1 and bla KPC-2 ) in a Klebsiella pneumoniae isolate that also had the PmrB(R256G) mutation associated with colistin resistance. The bla KPC-2 genes were located in Tn4401a transposons, while the bla NDM-1 genes were in the genetic structure Tn125 (ΔISAba125). The presence of high-risk clones among Klebsiella pneumoniae (ST11 and ST147) and Escherichia coli (ST410) isolates is also reported. The study reveals the emergence of highly resistant bacteria in a Peruvian hospital, which could compromise the effectiveness of current treatments and control.

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Effect of Wastewater Irrigation on the Presence of Extended Spectrum Beta-Lactamase (Esbl) Producing Escherichia Coli in Agricultural Soil

Rosas-Perez,

Lüneberg,

Aguilar-Rodea

et al. 2024

Preprint

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Genomic data reveals the emergence of the co-occurrence of blaKPC-2 and blaCTX-M-15 in an Escherichia coli ST648 strain isolated from rectal swab within the framework of hospital surveillance

Cited by 3 publications

References 6 publications

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Molecular characterization of carbapenemase-producing Enterobacterales in a tertiary hospital in Lima, Peru

Effect of Wastewater Irrigation on the Presence of Extended Spectrum Beta-Lactamase (Esbl) Producing Escherichia Coli in Agricultural Soil

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