Genomic discovery of ion channel genes in the central nervous system of the lamprey Petromyzon marinus

Northcutt, Adam J.; Hough, Ryan A.; Frese, Alexander N.; McClellan, Andrew D.; Schulz, David J.

doi:10.1016/j.margen.2019.03.003

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…In lamprey, two Nav1 paralogs that conserve ABM (XP_032802055 and XP_032831283 in Fig. 1 B ) are mainly expressed in the nervous system, and other three paralogs that only weakly conserve ABM show different expression patterns ( 27 , 33 , 55 , 56 ). It was also noted that these lamprey Nav1 paralogs are not necessarily grouped with either of mammalian four Nav1 clades: Nav1.1/1.2/1.3/1.7, Nav1.4, Nav1.5/1.8/1.9, and Nav1.6 ( 27 , 55 , 56 ).…”

Section: Discussionmentioning

confidence: 99%

“…1 B ) are mainly expressed in the nervous system, and other three paralogs that only weakly conserve ABM show different expression patterns ( 27 , 33 , 55 , 56 ). It was also noted that these lamprey Nav1 paralogs are not necessarily grouped with either of mammalian four Nav1 clades: Nav1.1/1.2/1.3/1.7, Nav1.4, Nav1.5/1.8/1.9, and Nav1.6 ( 27 , 55 , 56 ). These suggest that gene diversification from the CiNav1a-like ancestral Nav1 occurred independently in lamprey, and cell-type specific functional role was assigned to each gene, similarly to what presumably happened in the lineages of amphioxus, ascidians, and jawed vertebrates ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

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Heterologous functional expression of ascidian Nav1 channels and close relationship with the evolutionary ancestor of vertebrate Nav channels

Kawai

Hashimoto

Eguchi

et al. 2021

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heterologous functional expression of ascidian Nav1 channels and close relationship with the evolutionary ancestor of vertebrate Nav channels

Kawai

Hashimoto

Eguchi

et al. 2021

Journal of Biological Chemistry

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“…Furthermore, even if the present model were updated in the future based on new data, it is very likely the present study would still yield the same two main conclusions: multiple solutions in parameter space and correlations between ion channel conductances. Second, a recent molecular study identified a large number of voltage-gated and non-voltage-gated ion channels that were expressed in lamprey brain tissue containing reticular nuclei (Northcutt et al 2019). However, it is not clear whether RS neurons contain all of these ion channels, and if so, the locations and densities of the various channels in these cells currently are unknown.…”

Section: Dual Annealing and Grid Searches In The Present Studymentioning

confidence: 99%

Modeling of lamprey reticulospinal neurons: multiple distinct parameter sets yield realistic simulations

Ruffolo

McClellan

2020

Journal of Neurophysiology

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For the lamprey and other vertebrates, reticulospinal (RS) neurons project descending axons to the spinal cord and activate motor networks to initiate locomotion and other behaviors. In the present study, a biophysically-detailed computer model of lamprey RS neurons was constructed consisting of three compartments: dendritic; somatic; and axon initial segment (AIS). All compartments included passive channels. In addition, the soma and axon initial segment (AIS) had fast potassium and sodium channels. The soma included three additional voltage-gated ion channels (slow sodium, high-voltage activated (HVA) and low-voltage activated (LVA) calcium), and calcium-activated potassium (SK) channels. An initial manually-adjusted default parameter set, which was based in part on modified parameters from models of lamprey spinal neurons, generated simulations of single action potentials and repetitive firing that scored favorably (0.658; max=0.964) compared to experimentally-derived properties of lamprey RS neurons. Subsequently, a dual-annealing search paradigm identified 4302 viable parameter sets at local maxima within parameter space that yielded higher scores than the default parameter set, including many with much higher scores of ~0.85-0.87 (i.e. ~30% improvement). In addition, five-conductance and two-conductance grid searches identified a relatively large number of viable parameters sets for which significant correlations were present between maximum conductances for pairs of ion channels. The present results indicated that multiple model parameter sets ("solutions") generated action potentials and repetitive firing that mimicked many of the properties of lamprey RS neurons. To our knowledge, this is the first study to systematically explore parameter space for a biophysically-detailed model of lamprey RS neurons.

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Further studies of ion channels in the electroreceptor of the skate through deep sequencing, cloning and cross species comparisons

Clusin

Shi

et al. 2019

Gene

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Genomic discovery of ion channel genes in the central nervous system of the lamprey Petromyzon marinus

Cited by 5 publications

References 45 publications

Heterologous functional expression of ascidian Nav1 channels and close relationship with the evolutionary ancestor of vertebrate Nav channels

Heterologous functional expression of ascidian Nav1 channels and close relationship with the evolutionary ancestor of vertebrate Nav channels

Modeling of lamprey reticulospinal neurons: multiple distinct parameter sets yield realistic simulations

Further studies of ion channels in the electroreceptor of the skate through deep sequencing, cloning and cross species comparisons

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