Genomic dissection of Australian Bordetella pertussis isolates from the 2008–2012 epidemic

Safarchi, Azadeh; Octavia, Sophie; Wu, Sunny Z.; Kaur, Sandeep; Sintchenko, Vitali; Gilbert, Gwendolyn L.; Wood, Nicholas; McIntyre, Peter; Marshall, Helen; Keil, Anthony D.; Lan, Ruiting

doi:10.1016/j.jinf.2016.01.005

Cited by 43 publications

(64 citation statements)

References 66 publications

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“…Our previous comparative genome study identified several SNP differences between cluster I and cluster II strains at the genomic level including a potential adaptive SNP in subtilisin (SphB1) . Furthermore, our study and others also identified a region of difference (BP1948–BP1966 [RD10]) that was deleted in all ptxP3 cluster I strains but not in cluster II .…”

Section: Introductionsupporting

confidence: 57%

“…In BP3303, which encodes an ADP‐dependent (S)‐NAD(P)H‐hydrate dehydratase, L1423 contains a non‐synonymous SNP at nucleotide position 699 which results in an amino acid change from glycine to arginine. This SNP was also found in most Australian cluster I 2008–2012 epidemic strains that were sequenced (19/22) . Using SWISS‐MODEL and the site directed mutagenesis (SDM) server, the amino acid change in BP3303 was predicted to produce a protein with reduced stability (∆∆G = −2.2).…”

Section: Discussionmentioning

confidence: 99%

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Proteomic Adaptation of Australian Epidemic Bordetella pertussis

et al. 2018

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Bordetella pertussis causes whooping cough. The predominant strains in Australia changed to single nucleotide polymorphism (SNP) cluster I (pertussis toxin promoter allele ptxP3/pertactin gene allele prn2) from cluster II (non-ptxP3/non-prn2). Cluster I was mostly responsible for the 2008-2012 Australian epidemic and was found to have higher fitness compared to cluster II using an in vivo mouse competition assay, regardless of host's immunization status. This study aimed to identify proteomic differences that explain higher fitness in cluster I using isobaric tags for relative and absolute quantification (iTRAQ), and high-resolution multiple reaction monitoring (MRM-hr). A few key differences in the whole cell and secretome were identified between the cluster I and II strains tested. In the whole cell, nine proteins were upregulated (>1.2 fold change, q < 0.05) and three were downregulated (<0.8 fold change, q < 0.05) in cluster I. One downregulated protein was BP1569, a TLR2 agonist for Th1 immunity. In the secretome, 12 proteins were upregulated and 1 was downregulated which was Bsp22, a type III secretion system (T3SS) protein. Furthermore, there was a trend of downregulation in three T3SS effectors and other virulence factors. Three proteins were upregulated in both whole cell and supernatant: BP0200, molybdate ABC transporter (ModB), and tracheal colonization factor A (TcfA). Important expression differences in lipoprotein, T3SS, and transport proteins between the cluster I and II strains were identified. These differences may affect immune evasion, virulence and metabolism, and play a role in increased fitness of cluster I.

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Section: Introductionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Proteomic Adaptation of Australian Epidemic Bordetella pertussis

et al. 2018

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“…Furthermore, in the SNP cluster I strain used in this study, L1423, there were two additional genes with SNPs including sphB1 and fliM which are also under the Bvg regulation (Supplementary Table 1). The SNP in sphB1 was a common SNP found in all Australian B. pertussis SNP profile 13 (cluster I) isolates from the 2008-2012 epidemic that were sequenced previously while the SNP in fliM was only found in a subset of the isolates [15]. Genomic content of currently circulating B. pertussis strains has been reduced compared to other SNP clusters [38][39][40].…”

Section: Discussionmentioning

confidence: 97%

“…Both isolates expressed all antigens included in the ACV as shown previously [14]. The two isolates were also sequenced using the methods as detailed in Safarchi et al [15] (Supplementary File 1).…”

Section: B Pertussis Clinical Strainsmentioning

confidence: 99%

Better colonisation of newly emerged Bordetella pertussis in the co-infection mouse model study

Safarchi

Octavia

Luu

et al. 2016

Vaccine

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“…The session on biology, genomics, and evolution encompassed functional genomics, evolutionary genomics, and vaccine antigen deficiency of B. pertussis isolates that probably resulted from selection pressure from vaccine-induced immunity (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). New transcriptomics data expanded our understanding of gene regulation and its complexity in Bordetella biology.…”

Section: Biology Genomics and Evolutionmentioning

confidence: 99%

Highlights of the 11th International Bordetella Symposium: from Basic Biology to Vaccine Development

Carbonetti

König

Lan

et al. 2016

Clin Vaccine Immunol

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Pertussis is a severe respiratory disease caused by infection with the bacterial pathogen Bordetella pertussis. The disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. Other Bordetella species cause diseases in humans, animals, and birds. Scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in Buenos Aires, Argentina from 5 to 8 April 2016 for the 11th International Bordetella Symposium to discuss recent advances in our understanding of the biology of these organisms, the diseases they cause, and the development of new vaccines and other strategies to prevent these diseases. Highlights of the meeting included pertussis epidemiology in developing nations, genomic analysis of Bordetella biology and evolution, regulation of virulence factor expression, new model systems to study Bordetella biology and disease, effects of different vaccines on immune responses, maternal immunization as a strategy to prevent newborn disease, and novel vaccine development for pertussis. In addition, the group approved the formation of an International Bordetella Society to promote research and information exchange on bordetellae and to organize future meetings. A new Bordetella.org website will also be developed to facilitate these goals.

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Genomic dissection of Australian Bordetella pertussis isolates from the 2008–2012 epidemic

Cited by 43 publications

References 66 publications

Proteomic Adaptation of Australian Epidemic Bordetella pertussis

Proteomic Adaptation of Australian Epidemic Bordetella pertussis

Better colonisation of newly emerged Bordetella pertussis in the co-infection mouse model study

Highlights of the 11th International Bordetella Symposium: from Basic Biology to Vaccine Development

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