Genomic diversities of <scp>ctxB</scp>, <scp>tcpA</scp> and <scp>rstR</scp> alleles of Vibrio cholerae <scp>O139</scp> strains isolated from Odisha, India

Behera, Dipti Ranjan; Nayak, Ashish Kumar; Nayak, Smruti Ranjan; Nayak, Dilena; Swain, Sipraswati; Maharana, Pradeep Kumar; Biswal, Bhagyalaxmi; Pany, Swatishree; Pal, Bibhuti Bhusan

doi:10.1111/1758-2229.13016

Cited by 4 publications

(1 citation statement)

References 35 publications

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“… 21 V. cholerae O1 and O139, which cause cholera, generally have CTX genes. 22 The virulence genes of the strain reported in this paper are tox R, RTX, hlyA, T3SS/T6SS , and lack of CTX gene, ace gene, and zot gene. Compared with O1 or O139, the virulence genes of V. cholerae are less, and the strain does not produce cholera toxin.…”

Section: Discussionmentioning

confidence: 78%

Whole Genome Analysis of a Non-O1, Non-O139 Vibrio cholerae Detected from Human Blood in China

Tang,

Li,

Zhang

et al. 2023

IDR

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Non-O1, non-O139 Vibrio cholerae (NOVC) can cause cholera-like diarrhea, but it rarely causes extraintestinal infection, so it is easily overlooked. In this report, we present a case of NOVC detected through blood culture in a 58-year-old male patient with cirrhosis, resulting in severe infection. The patient had been diagnosed with cirrhosis seven years prior and was admitted to the hospital due to abdominal distension and gastrointestinal bleeding. Gram-negative bacilli were isolated from blood cultures and identified as V. cholerae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and average nucleotide identity (ANI). Moreover, the serum agglutination test showed that the strain was non-O1/non-O139. Further whole genome sequencing and analysis of the strain showed that the strain mainly carried virulence genes tox R, RTX, hly A, T3SS/T6SS , but no resistant genes such as sulII, dfrA1, strB were detected. It provides information for the study of the pathogenic mechanism and drug resistance mechanism of V. cholerae . The patient had severe symptoms and a poor prognosis, indicating that although the NOVC strain infected in this patient had few virulence genes, it was not weak in pathogenicity. It may be caused by the effect of some virulence genes, which should be paid attention to.

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Section: Discussionmentioning

confidence: 78%

Whole Genome Analysis of a Non-O1, Non-O139 Vibrio cholerae Detected from Human Blood in China

Tang,

Li,

Zhang

et al. 2023

IDR

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The assessment of microbial ecology: a special emphasis on the Indian scenario

Lal

Singh

Sarkar

et al. 2022

Environ Microbiol Rep

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Spectrum of ctxB genotypes, antibiogram profiles and virulence genes of Vibrio cholerae serogroups isolated from environmental water sources from Odisha, India

et al. 2023

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Background The present study reports on the comprehensive analysis of Vibrio cholerae O1 and non-O1/non-O139 serogroups isolated from environmental water sources during cholera outbreaks, epidemics and surveillance studies between years 2007 to 2019 from different districts of Odisha, India. Methods A total of 85 stocked cultures of V. cholerae O1 and non-O1/non-O139 strains were analyzed for different ctxB genotypes, toxic genes, antibiogram profiles through PCR assays and pulsotyped by pulsed-field gel electrophoresis (PFGE). Results From all V. cholerae strains tested, 51 isolates were O1 Ogawa and the rest 34 strains were non-O1/non-O139. All the V. cholerae O1 strains were altered El Tor variants carrying ctxB1, ctxB3 and ctxB7 genotypes. However, only ctxB1 genotypes were present in V. cholerae non-O1/non-O139. Though non-O1/non-O139 strains were negative by O1 antisera, 20% strains were positive for rfbO1 gene by PCR assay. All the V. cholerae isolates possessed a variety of virulence genes including ace, ctxAB, toxR, zot, hlyA which were in higher percentage in the case of V. cholerae O1. The Vibrio cholerae O1 and non-O1-/non-O139 strains showed multiple antibiotic resistances in 2007 and 2012. The PCR detection of four resistance associated genes (strB, dfrA1, sulll, SXT) confirmed higher prevalence in V. cholerae non-O1/non-O139 strains. The PFGE analysis revealed 3 pulsotypes having 93% similarity among V. cholerae O1 strains. Conclusion This study indicates the changing epidemiology, antibiogram patterns and continuous genetic variation in environmental V. cholerae strains of Odisha over the years. So continuous surveillance is necessary to understand the changing patterns of V. cholerae different serogroups isolated from stool and water samples from Odisha.

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Genomic diversities of ctxB, tcpA and rstR alleles of Vibrio cholerae O139 strains isolated from Odisha, India

Cited by 4 publications

References 35 publications

Whole Genome Analysis of a Non-O1, Non-O139 Vibrio cholerae Detected from Human Blood in China

Whole Genome Analysis of a Non-O1, Non-O139 Vibrio cholerae Detected from Human Blood in China

The assessment of microbial ecology: a special emphasis on the Indian scenario

Spectrum of ctxB genotypes, antibiogram profiles and virulence genes of Vibrio cholerae serogroups isolated from environmental water sources from Odisha, India

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