Genomic Diversity of Methicillin-Resistant Staphylococcus aureus CC398 Isolates Collected from Diseased Swine in the German National Resistance Monitoring Program GE
            <i>RM</i>
            -Vet from 2007 to 2019

Krüger-Haker, Henrike; Ji, Xing; Hanke, Dennis; Fiedler, S.; Feßler, Andrea T.; Jiang, Nansong; Kaspar, Heike; Wang, Yan; Wu, Congming; Schwarz, Štefan

doi:10.1128/spectrum.00770-23

Cited by 9 publications

(2 citation statements)

References 96 publications

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“…Recent reports have identified CC398 as the predominant LA-MRSA clone found in livestock worldwide, including Europe, North America, Asia, and Africa ( Wang et al, 2023 ). LA-MRSA-CC398, lacking specific host specificity, can cross various species barriers, colonize or infect animals and humans through close contact with contaminated livestock or food, leading to human infections ( Tang et al, 2021 ; Kruger-Haker et al, 2023 ). Due to the stable presence of Tn916 and SCCmec in CC398, these mobile genetic elements (or other antibiotic resistance genes) are often retained when CC398 was transmitted to humans ( Matuszewska et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Huang,

Zhu,

Yan

et al. 2024

Front. Microbiol.

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Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen contributing to healthcare-associated infections, which can result in multiple sites infections. The epidemiological characteristics of MRSA exhibit variability among distinct regions and healthcare facilities. The aim of this study was to investigate the molecular epidemiology and nosocomial outbreak characteristics of MRSA in a county-level hospital in China. A total of 130 non-repetitive MRSA strains were collected from December 2020 to November 2021. Whole-genome sequencing (WGS) was performed to identify antimicrobial resistance and virulence factors. Phylogenetic analysis was conducted to ascertain genetic diversity and phylogenetic relationships. Independent transmission scenarios were determined by the phylogeny derived from single nucleotide polymorphisms (SNPs) within the core genome. All the MRSA isolates were collected from the intensive care unit (30.00%, 39/130), the department of otorhinolaryngology (10.00%, 13/130) and the department of burn unit (9.23%, 12/130). The clinical samples mainly included phlegm (53.85%, 70/130), purulent fluid (24.62%, 32/130), and secretions (8.46%, 11/130). The resistance rates to erythromycin, clindamycin and ciprofloxacin were 75.38, 40.00, and 39.23%, respectively. All the isolates belonged to 11 clonal complexes (CCs), with the major prevalent types were CC5, CC59, and CC398, accounting for 30.00% (39/130), 29.23% (38/130), and 16.92% (22/130), respectively. Twenty sequence types (STs) were identified, and ST59 (25.38%, 33/130) was the dominant lineage, followed by ST5 (23.84%, 31/130) and ST398 (16.92%, 22/130). Three different SCCmec types were investigated, most of isolates were type IV (33.85%, 44/130), followed by type II (27.69%, 36/130) and type III (0.77%, 1/130). The common clonal structures included CC5-ST5-t2460-SCCmec IIa, CC59-ST59-t437-SCCmec IV and CC398-ST398-t034-SCCmec (−), with rates of 16.92% (22/130), 14.62% (19/130), and 13.84% (18/130), respectively. Only 12 panton-valentine leucocidin (PVL) positive strains were identified. Two independent clonal outbreaks were detected, one consisting of 22 PVL-negative strains belongs to CC5-ST5-t2460-SCCmec IIa and the other consisting of 8 PVL-negative strains belongs to CC5-ST5-t311-SCCmec IIa. Overall, our study indicated that the CC5 lineage emerged as the predominant epidemic clone of MRSA, responsible for nosocomial outbreaks and transmission within a county-level hospital in China, highlighting the necessity to strengthen infection control measures for MRSA in such healthcare facilities.

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Section: Discussionmentioning

confidence: 99%

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Huang,

Zhu,

Yan

et al. 2024

Front. Microbiol.

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“…However, some studies have demonstrated that IEC harbouring LA-MRSA CC398 can be present in animal isolates, and it has also been proposed that LA-MRSA CC398 is capable of re-adapting to the human host (Cuny et al 2015a;Sieber et al 2020;Avberšek et al 2021). However, virulence factors, such as enterotoxin, exotoxin and exfoliative toxin genes, have also been reported, and LA-MRSA CC383 can acquire foreign genetic material by horizontal gene transfer (Krüger-Haker et al 2023). The capability of reobtaining virulence factors and the recent evolution of MRSA CC398 of human origin might pose a greater risk to human and animal health in the future.…”

Section: Evolution Of La-mrsa Cc398 and Other Mrsa Cc398mentioning

confidence: 99%

Control strategies for LA-MRSA in Swedish pig production: A disease modelling approach

Tuominen¹

2023

Acta Universitatis Agriculturae Sueciae

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Antimicrobial resistance is a threat to global health. Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a category of multiresistant bacteria that primarily colonises livestock animals. While pigs are considered its main reservoir, LA-MRSA is zoonotic and causes an occupational risk to those working with livestock. However, LA-MRSA is also capable of spreading to humans without livestock contact. Although LA-MRSA carriers are usually asymptomatic, LA-MRSA can cause a wide range of infections in humans. The aim of this thesis was to use disease modelling to study the spread of LAMRSA and assess possible control strategies in a Swedish farrow-to-finish pig herd—additionally, the thesis aimed to fill knowledge gaps regarding the survival of LA-MRSA in the farm environment. The modelling studies concluded that eradicating LA-MRSA is challenging. Early detection and introduction of control measures were considerably more effective in lowering the within-herd prevalence than measures that were implemented when LA-MRSA had become established in the herd. The time to disease elimination was at least 300 days even with the most effective control measures that were introduced early at the outbreak phase of disease spread. In an experimental study, the survival of LA-MRSA strains belonging to the clonal complex (CC) 398 varied on different surface materials (concrete, polypropylene plastic and stainless steel). This finding can be beneficial when planning efficient cleaning and disinfection routines in pig farms. In conclusion, investing in early detection and intensive early control measures may be justified if a low LA-MRSA prevalence country aims to have LA-MRSAfree pig herds.

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A look at staphylococci from the one health perspective

Cuny,

Layer-Nicolaou,

Werner

et al. 2024

International Journal of Medical Microbiology

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Genomic Diversity of Methicillin-Resistant Staphylococcus aureus CC398 Isolates Collected from Diseased Swine in the German National Resistance Monitoring Program GE RM -Vet from 2007 to 2019

Cited by 9 publications

References 96 publications

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Control strategies for LA-MRSA in Swedish pig production: A disease modelling approach

A look at staphylococci from the one health perspective

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