Genomic diversity of oral <i>Candida krusei</i> isolates as revealed by DNA fingerprinting and electrophoreric karyotyping

Dassanayake, R.S.; Samaranayake, Y. H.; Samaranayake, LP

doi:10.1034/j.1600-0463.2000.d01-17.x

Cited by 13 publications

(7 citation statements)

References 29 publications

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“…A total of 55 isolates recovered from 23 patients could be differentiated into 31 genotypes by using primer 1, which showed the highest discriminatory power with a discriminatory index of 0.977. Our data support previous findings that the discriminatory power of RAPD analysis rests upon the primer used (11).…”

Section: Discussionsupporting

confidence: 92%

Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre‐school children

Hossain¹,

Ansari

Schulz‐Weidner³

et al. 2003

Oral Microbiology and Immunology

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The clonal relationship between oral and fecal Candida albicans isolated from children of pre-school age was examined using RAPD analysis. Significantly higher levels of C. albicans were found in saliva, dental plaque, carious specimens and stools of 56 patients with severe caries as compared to 52 healthy control subjects. The highest prevalence was found in carious specimens and a strong correlation was observed between its presence in saliva, dental plaque, carious specimen and feces. RAPD analysis of isolates from 23 patients with simultaneous oral and fecal C. albicans revealed clonal counterparts present in both oral and stool samples in 15 cases; five patients harbored closely related strains; and three patients harbored unrelated strains. Our results demonstrate a strong correlation between oral and gastrointestinal C. albicans colonization. We assume that carious teeth may constitute an ecologic niche for C. albicans potentially responsible for recurrent oral and non-oral candidiasis.

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Section: Discussionsupporting

confidence: 92%

Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre‐school children

Hossain¹,

Ansari

Schulz‐Weidner³

et al. 2003

Oral Microbiology and Immunology

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“…Karyotypic comparison of seven nonisogenic P. anomala strains, characterized as P. anomala by BCCM TM /MUCL, revealed chromosomal length polymorphism, a sign of intraspecies variation, as already observed for other yeast species such as Candida krusei (Dassanayake et al. 2000), Candida albicans (Mehta et al.…”

Section: Discussionsupporting

confidence: 71%

Genetic characterization of the yeast Pichia anomala (strain K), an antagonist of postharvest diseases of apple

2005

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Aims:To obtain information about the genomic organization of Pichia anomala (strain K) and about its genomic diversity at species and intraspecies level. Methods and Results: The PFGE karyotype of strain K was composed of four bands ranging in size from 1AE1 to 3AE2 Mb. The number of chromosomes was estimated at six since bands 2 and 3 seemed to result from the comigration of two chromosomes with similar size. A comparison of strain K and Hansenula wingeii migration profiles led to the estimate of K strain genome size at 11AE7 Mb. Comparison with isogenic strains, resulting from the sporulation of strain K, highlighted some major karyotypic differences. Two segregants (KH6 and KH7) showed supernumerary chromosomes and one (KH9) displayed chromosomal length polymorphism. This genomic instability was confirmed by molecular hybridization with four probes, consisting of URA3, LEU2, PAEXG1 and PAEXG2 genes of P. anomala. URA3 and LEU2 probes showed second hybridization signals on supernumerary chromosomes of strain KH7 and on chromosome 6 of strain K for LEU2 only. Karyotypic comparison of seven nonisogenic P. anomala strains revealed chromosomal length polymorphism, a sign of intraspecies variation. Conclusions: This work has supplied information about genome size and chromosome number of strain K of P. anomala. The strain seems to be aneuploid because of the presence of supernumerary chromosomes and additional hybridization signals for URA3 and LEU2 probes in the chromosomal profile of some segregants. The work also highlighted genomic diversity within the P. anomala species. Significance and Impact of the Study: Results obtained here increase information about the aneuploidy of P. anomala (strain K). Information about the genomic diversity of the segregants will be of great interest for further studies on strain K mode of action. The genome size and chromosomal profile of P. anomala presented here are different from the results obtained elsewere for Hansenula anomala, while Hansenula is included as a synonym of Pichia. This warrants further studies to investigate this taxonomic relationship.

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“…PFGE was used previously to estimate the number of chromosomes for clinical and environmental isolates of C. krusei ( Iwaguchi et al 1990 ; Doi et al 1992 ; Dassanayake et al 2000 ; Jespersen et al 2005 ). Based on the chromosomal patterns, it was estimated that C. krusei has a total of four to six chromosomes: around two to four large chromosomes (∼2.8–3.5 Mb), and two small chromosomes (∼1.4 Mb).…”

Section: Resultsmentioning

confidence: 99%

Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5

Cuomo¹,

Shea

Yang

et al. 2017

G3 Genes|Genomes|Genetics

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Candida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here, we report a high-quality genome sequence and assembly for the first clinical isolate of C. krusei, strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that, while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen.

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Genomic diversity of oral Candida krusei isolates as revealed by DNA fingerprinting and electrophoreric karyotyping

Cited by 13 publications

References 29 publications

Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre‐school children

Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre‐school children

Genetic characterization of the yeast Pichia anomala (strain K), an antagonist of postharvest diseases of apple

Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5

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