Genomic Exploration of the Hemiascomycetous Yeasts: 9. <i>Saccharomyces kluyveri</i>

Neuvéglise, Cécile; Bon, Elisabeth; Lépingle, Andrée; Wincker, Patrick; Artiguenave, François; Gaillardin, Claude; Casarégola, Serge

doi:10.1016/s0014-5793(00)02280-8

Cited by 27 publications

(19 citation statements)

References 33 publications

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“…Among 682 couples of genes [29], we observed 56.5% conserved synteny between the two yeasts. This value is close to S. kluyveri (53.5%) [30], K. lactis (47.3%) [31], and K. marxianus var. marxianus (49.8%) [32].…”

Section: 7supporting

confidence: 57%

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

et al. 2000

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A genomic exploration of Kluyveromyces thermotolerans was performed by random sequence tag (RST) analysis. We sequenced 2653 RSTs corresponding to inserts sequenced from both ends. We performed a systematic comparison with a complete set of proteins from Saccharomyces cerevisiae, other completely sequenced genomes and SwissProt. We identified six mitochondrial genes and 1358^1496 nuclear genes by comparison with S. cerevisiae. In addition, 25 genes were identified by comparison with other organisms. This corresponds to about 24% of the estimated gene content of this organism. A lower level of conservation is observed with orthologues to genes of S. cerevisiae previously classified as orphans. Gene order was found to be conserved between S. cerevisiae and K. thermotolerans in 56.5% of studied cases. ß

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Section: 7supporting

confidence: 57%

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

et al. 2000

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“…Genes present in paralogous gene families represent only 4.5% of the newly identi¢ed genes in S. servazzii. This is much less than what was observed in other species [21,22]. Among the S. servazzii paralogues identi¢ed here, ORFs involved in transport are the most frequent.…”

Section: Duplicated Genescontrasting

confidence: 57%

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Casarégola¹,

Lépingle²,

Bon³

et al. 2000

FEBS Letters

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The genome of Saccharomyces servazzii was analyzed with 2570 random sequence tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 putative open reading frames with significant homology to Saccharomyces cerevisiae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S. cerevisiae. About 25% of the S. servazzii genes were identified, assuming that the gene complement is identical in both yeasts. S. servazzii carries very few transposable elements related to Ty elements in S. cerevisiae. Most of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb. A significant match with the Kluyveromyces lactis linear DNA plasmid pGKL-1 encoded RF4 killer protein suggests that a related plasmid exists in S. servazzii. The sequences have been deposited with EMBL under the accession numbers AL402279Â L404848. ß 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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“…As their counterparts in S. cerevisiae, the S. exiguus COX1 and COB genes exhibit a mosaic intronic structure, but the ai1, ai2, ai5K, ai5L, and ai5Q introns of COX1 [18] were not detected in S. exiguus. Similar observations have been reported for S. kluyveri and S. servazzii [12,16], suggesting a di¡erent organization of introns. Several intronic ORFs were also detected to be associated to COX1, COB and the 21S rRNA gene.…”

Section: Mtdnasupporting

confidence: 87%

“…DNA fragmentation and cloning, as well as the quality control of the library, were performed as described by Neuvëglise et al [12]. A set of 1389 clones was sequenced by Gënoscope [13].…”

Section: Library Constructionmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 6. Saccharomyces exiguus

Bon¹,

Neuvéglise²,

Lépingle³

et al. 2000

FEBS Letters

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Random sequence tags were obtained from a genomic DNA library of Saccharomyces exiguus. The mitochondrial genome appeared to be at least 25.7 kb in size, with a different organization compared to Saccharomyces cerevisiae. An unusual putative 953 bp long terminal repeated element associated to Ty3 was found. A set of 1451 genes was identified homologous to S. cerevisiae open reading frames. Only five genes were identified outside the S. cerevisiae taxon, confirming that S. exiguus is phylogenetically closely related to S. cerevisiae. Unexpectedly, numerous duplicated genes were found whereas they are unique in S. cerevisiae. The sequences are deposited at EMBL under the accession numbers: AL407377^AL409955. ß

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Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

Cited by 27 publications

References 33 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Genomic Exploration of the Hemiascomycetous Yeasts: 6. Saccharomyces exiguus

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