Genomic identification of Border disease virus in sheep aborted foetuses

Mokhtari, Azam; Manshoori, M.

doi:10.15547/bjvm.1054

Cited by 4 publications

(9 citation statements)

References 12 publications

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“…This may be contributed to absence of vaccination and/or control programs in Mosul city, despite the occurrence of BDV being documented in previous studies (20,21). A novel introduction of BDV to the farms in Iraq is due to the presence of imported sheep, and goats from Iraq's neighbors and other BDV-endemic countries such as Iran (27) and Turkey (28). This result agrees with what was mentioned by Hasan and Alsaad (29).…”

Section: Discussionsupporting

confidence: 84%

“…The outcomes from the current work indicated that the occurrence of BDV in Mosul was detected by utilizing a PCR technique are approximately the same or different from those indicated in other countries. In Turkey it was 22.2% (28), while, in Iran was 9% (27). In Spain the result ranged from 7.9-50% (37).…”

Section: Discussionmentioning

confidence: 91%

“…The analysis of final PCR products was applied using agarose gel electrophoresis and observable on 1.5% agarose gel stained with ethidium bromide and compared with DNA markers (100 base pair ladder, Axon Scientific Sdn Bhd, Malaysia). The RT-PCR procedural stages stated above were preformed according to the manufacturer's instructions and the procedure of (27).…”

Section: Rna Extraction and Rt-pcr Amplificationmentioning

confidence: 99%

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Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Hassan¹

2021

IJVS

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Globally, Border disease virus (BDV) has caused substantial economic losses among small ruminants (sheep and goats). This is the first molecular study carried out in Mosul city, Iraq. To determine the prevalence of Border Disease Virus and to examine problem of persistent infection (PI) using Reverse transcriptase polymerase chain reaction technique (RT-PCR) in female local breed of small ruminants. During the period between November 2018 to June 2019, 364 blood samples were collected from 264 local Awassi sheep and 100 local cross breed goats secure provided by private breeders. The animals were of ≥1.5 years old and the samples were obtained from various locations in Mosul city, with varying rearing methods and had not been vaccinated against BDV. This investigation indicated that the prevalence of BDV infection in sheep and goats were 15.9% (42/264) and 3% (3/100) respectively, whereas the occurrence of PI in sheep was 2.38% and in goats was 0%. Hence it was concluded that Border disease was circulating in small ruminants in Mosul city. This calls for a need to design programs to monitor and control the disease and eventually eradicate it is prevalence in Mosul city.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 91%

Section: Rna Extraction and Rt-pcr Amplificationmentioning

confidence: 99%

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Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Hassan¹

2021

IJVS

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“…′ UTR and BVDV-NS3 via infection with lentiviral vectors (24). Similar assessments were performed by Basagoiti et al (31) who developed a Western Nile virus (WNV) sub-replicon-expressing cell line.…”

Section: Discussionmentioning

confidence: 69%

“…There are no commercial live attenuated or recombinant subunit vaccines for BDV. The BVDV vaccine for cows is not recommended for use in sheep since border disease viruses, which are usually common in sheep population, are genetically different from the most common strains of BVDV ( 3 , 24 , 25 ). On the other hand, it is important to note that no systematic antiviral drugs have been reported for border disease; however, since BDV has a very close relationship with its family members, BVDV and CSFV, the drugs used for them could be implicated for BDV.…”

Section: Discussionmentioning

confidence: 99%

In vitro Inhibition of Border Disease Virus Replication With Lentivirus-Mediated shRNAs

et al. 2021

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Background: Border disease is believed to be one of the most important diseases in the animal husbandry industry, which has not yet been eradicated in Iran. The development of approaches based on the application of interfering RNA (RNAi) for antiviral therapy has attracted a great deal of attention over the recent years. The present research was conducted to design, construct, and apply shRNA against the NS3 gene of BDV to evaluate the prevention of BDV proliferation in the cell culture system. For this purpose, the suitable oligonucleotide sequence of NS3 gene coding was selected utilizing BDV- X818 strain. Afterwards, using shRNA design software, shRNA molecules were designed and synthesized. These shRNAs were cloned into the desired vectors and were finally transfected in HEK293T cells employing the third generation of lentiviral packaging system. Subsequently, these shRNA expressing lentiviruses were transduced to the MDBK cell line to challenge to border virus. In order to evaluate the efficacy of shRNAs, the viral infectious titer and RNA copy number were calculated with TCID50 and Real-time RT-PCR tests, respectively.Results: The results revealed that shRNAs 1, 2, and 3 decreased viral RNA by more than 90% compared to the control groups. BDV titer noticeably decreased after the challenge with shRNAs 1, 2, and 3 from ~88% up to 99% in comparison with the control groups.Conclusions: Overall, it could be concluded that RNAi may be considered as a strong treatment proposal against viruses, such as BDV.

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Causes of abortion in Iranian sheep flocks and associated risk factors

et al. 2022

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Abortion is a major issue in sheep husbandry. It can result in significant economic losses and a severe public health risk. This survey assessed the infectious and non-infectious causes of abortion in Iranian sheep flocks and determined the main risk factors. In this cross-sectional survey, causes of abortion were evaluated in 757 sheep flocks, and risk factors were analysed. A checklist containing general animal information for each abortion outbreak evaluated was filled in. Data were analysed using univariate tests and multivariable binary logistic regression analysis. In this sense, parity, gestational age of the aborted fetus, vaccination protocol, mineral supplementation and history of stillbirth showed significant associations with abortion. Infectious agents such as Coxiella burnetti (22.7%), Chlamydia abortus (12.3%) and Brucella melitensis (10.4%) were the most frequently isolated in the investigated flocks, with more than 2% of abortion rates. On the other hand, non-infectious agents such as trauma, pregnancy toxaemia and vitamin E/Se deficiency were involved in those flocks with low abortion rates (less than 10%). Results revealed multiple causes of abortion outbreaks among Iranian sheep flocks, which need careful investigation to identify possible aetiology and risk factors. Further studies are necessary to evaluate if these factors are similar to other countries in the same region.

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Genomic identification of Border disease virus in sheep aborted foetuses

Cited by 4 publications

References 12 publications

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

In vitro Inhibition of Border Disease Virus Replication With Lentivirus-Mediated shRNAs

Causes of abortion in Iranian sheep flocks and associated risk factors

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