Genomic-independent action of thyroid hormones on NTPDase activities in Sertoli cell cultures from congenital hypothyroid rats

Purinergic signalling in the reproductive system in health and disease

“…The effect of congenital hypothyroidism and thyroid hormone supplementation on NTPDase activities in Sertoli cells influences the actions of adenosine and ATP on reproductive functions throughout development [455].…”

Membrane-initiated actions of thyroid hormones on the male reproductive system

“…In this context, activation of P2X 7 purinergic receptor by ATP was considered to be important in increasing intracellular Ca 2+ levels in these cells (Ko et al, 2003). It has also been shown that thyroid hormones can inhibit extracellular ATP hydrolysis leading to increased levels of this nucleotide in Sertoli cells (Zamoner et al, 2006a). In this context, the Cl − secretion in response to Ca 2+ influx in Sertoli cells could be observed after ATP exposure and maybe after thyroid hormone treatment.…”

“…Several evidences support that thyroid hormones act mainly through their nuclear receptors in Sertoli cells, but also these hormones give rise to a wide range of effects in Sertoli cells lacking nuclear receptor activation (Silva et al, , 2002Volpato et al, 2004;Zamoner et al, 2005Zamoner et al, , 2006aMenegaz et al, 2006Menegaz et al, , 2010. These findings led to the identification of non-classical thyroid hormone binding elements in the plasma membrane of testicular cells (Silva et al, 2002).…”

“…These findings led to the identification of non-classical thyroid hormone binding elements in the plasma membrane of testicular cells (Silva et al, 2002). Through binding to these sites, thyroid hormones could exert nongenomic effects, including those on ion fluxes at the plasma membrane (Zamoner et al, 2005;Menegaz et al, 2010), signal transducing pathways (Zamoner et al, 2007b(Zamoner et al, , 2008aMenegaz et al, 2010), amino acid accumulation Volpato et al, 2004;Menegaz et al, 2006Menegaz et al, , 2010, calcium influx (Menegaz et al, 2010), modulation of extracellular nucleotide levels (Zamoner et al, 2006a) and alteration in the intermediate filament cytoskeleton dynamics (Zamoner et al, 2005). Although there are several reports of extranuclear actions of thyroid hormones in male reproductive system, the membrane receptor involved in these actions has not yet been identified in testicular cells.…”

Purinergic signalling in endocrine organs

“…Evidence for sympathetic innervation of human Leydig cells has been presented and their influence on the secretion of testosterone, Thyroid hormones are regulators of the male reproductive system. They modulate extracellular ATP levels in hypothyroid cultured Sertoli cells and congenital hypothyroidism and thyroid hormone supplementation on NTPDase activities in Sertoli cells can influence the actions of ATP and adenosine on reproductive functions during development [611].…”