Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817

Gao, Dandan; Chang, Kunpeng; Ding, Gongtao; Wu, Hongjing; Chen, Yuanhong; Jia, Mengya; Liu, Xiaohua; Wang, Shuixing; Jin, Yuyuan; Pan, Hao; Li, Haixing

doi:10.1186/s13568-019-0799-0

Cited by 40 publications

(27 citation statements)

References 58 publications

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“…brevis strains, with only 91% of amino acid sequence identity. The results further demonstrated that GadB contributed to the main GAD activity [55]. gadA does not have a close relationship with the accumulation of GABA.…”

Section: Gad System Of Labmentioning

confidence: 78%

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Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Cui

Miao

Niyaphorn

et al. 2020

IJMS

289

186

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Gamma-aminobutyric acid (GABA) is widely distributed in nature and considered a potent bioactive compound with numerous and important physiological functions, such as anti-hypertensive and antidepressant activities. There is an ever-growing demand for GABA production in recent years. Lactic acid bacteria (LAB) are one of the most important GABA producers because of their food-grade nature and potential of producing GABA-rich functional foods directly. In this paper, the GABA-producing LAB species, the biosynthesis pathway of GABA by LAB, and the research progress of glutamate decarboxylase (GAD), the key enzyme of GABA biosynthesis, were reviewed. Furthermore, GABA production enhancement strategies are reviewed, from optimization of culture conditions and genetic engineering to physiology-oriented engineering approaches and co-culture methods. The advances in both the molecular mechanisms of GABA biosynthesis and the technologies of synthetic biology and genetic engineering will promote GABA production of LAB to meet people’s demand for GABA. The aim of the review is to provide an insight of microbial engineering for improved production of GABA by LAB in the future.

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Section: Gad System Of Labmentioning

confidence: 78%

“…Recently, it was found that a high GABA-producing Lb. brevis CD0817 contained only a gadB-gadC operon and was absent of gadA gene [55]. What is more, there are obvious differences between GadB of CD0817 and those in the other GABA-producing Lb.…”

Section: Gad System Of Labmentioning

confidence: 96%

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Cui

Miao

Niyaphorn

et al. 2020

IJMS

289

186

View full text Add to dashboard Cite

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“…Typically, L. brevis contains two GAD-encoding genes, gadA and gadB, which when expressed yield GAD enzymes that share approximately 50% amino acid sequence similarity [6]. In contrast, the gadB gene is absent in strain L. brevis CD0817 [59] and the amino acid sequence identities of GadA and GadC from L. brevis CD0817 against other L. brevis strains are 91% and 90%, respectively. The transcriptional regulator gene gadR plays a crucial role in GABA production and acid resistance in L. brevis.…”

Section: Occurrence and Organization Of Glutamic Acid Decarboxylase (Gad) Genesmentioning

confidence: 99%

Glutamate Decarboxylase from Lactic Acid Bacteria—A Key Enzyme in GABA Synthesis

2020

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Glutamate decarboxylase (l-glutamate-1-carboxylase, GAD; EC 4.1.1.15) is a pyridoxal-5’-phosphate-dependent enzyme that catalyzes the irreversible α-decarboxylation of l-glutamic acid to γ-aminobutyric acid (GABA) and CO2. The enzyme is widely distributed in eukaryotes as well as prokaryotes, where it—together with its reaction product GABA—fulfils very different physiological functions. The occurrence of gad genes encoding GAD has been shown for many microorganisms, and GABA-producing lactic acid bacteria (LAB) have been a focus of research during recent years. A wide range of traditional foods produced by fermentation based on LAB offer the potential of providing new functional food products enriched with GABA that may offer certain health-benefits. Different GAD enzymes and genes from several strains of LAB have been isolated and characterized recently. GABA-producing LAB, the biochemical properties of their GAD enzymes, and possible applications are reviewed here.

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“…Microorganisms, including LAB, are an important source of GABA [ 1 , 39 – 42 ], and the GABA producing capability amongst LAB like lactobacilli varies between species and strains [ 43 – 47 ]. More recently, high GABA producing lactobacilli strains have been isolated to meet consumer needs [ 45 , 48 ]. In this manuscript, we sought to further investigate strain-specific mechanisms for GABA production in Levilactobacillus brevis .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics reveal different metabolic strategies for acid resistance and gamma-aminobutyric acid (GABA) production in select Levilactobacillus brevis strains

Banerjee¹,

Poore²,

Gerdes³

et al. 2021

Microb Cell Fact

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Background Of the many neurotransmitters in humans, gamma-aminobutyric acid (GABA) shows potential for improving several mental health indications such as stress and anxiety. The microbiota-gut-brain axis is an important pathway for GABAergic effects, as microbially-secreted GABA within the gut can affect host mental health outcomes. Understanding the molecular characteristics of GABA production by microbes within the gut can offer insight to novel therapies for mental health. Results Three strains of Levilactobacillus brevis with syntenous glutamate decarboxylase (GAD) operons were evaluated for overall growth, glutamate utilization, and GABA production in typical synthetic growth media supplemented with monosodium glutamate (MSG). Levilactobacillus brevis Lbr-6108™ (Lbr-6108), formerly known as L. brevis DPC 6108, and Levilactobacillus brevis Lbr-35 ™ (Lbr-35) had similar growth profiles but differed significantly in GABA secretion and acid resistance. Lbr-6108 produced GABA early within the growth phase and produced significantly more GABA than Lbr-35 and the type strain Levilactobacillus brevis ATCC 14869 after the stationary phase. The global gene expression during GABA production at several timepoints was determined by RNA sequencing. The GAD operon, responsible for GABA production and secretion, activated in Lbr-6108 after only 6 h of fermentation and continued throughout the stationary phase. Furthermore, Lbr-6108 activated many different acid resistance mechanisms concurrently, which contribute to acid tolerance and energy production. In contrast, Lbr-35, which has a genetically similar GAD operon, including two copies of the GAD gene, showed no upregulation of the GAD operon, even when cultured with MSG. Conclusions This study is the first to evaluate whole transcriptome changes in Levilactobacillus brevis during GABA production in different growth phases. The concurrent expression of multiple acid-resistance mechanisms reveals niche-specific metabolic functionality between common human commensals and highlights the complex regulation of GABA metabolism in this important microbial species. Furthermore, the increased and rapid GABA production of Lbr-6108 highlights the strain’s potential as a therapeutic and the overall value of screening microbes for effector molecule output.

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Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817

Cited by 40 publications

References 58 publications

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Glutamate Decarboxylase from Lactic Acid Bacteria—A Key Enzyme in GABA Synthesis

Transcriptomics reveal different metabolic strategies for acid resistance and gamma-aminobutyric acid (GABA) production in select Levilactobacillus brevis strains

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