Genomic insights of Klebsiella pneumoniae isolated from a native Amazonian fish reveal wide resistome against heavy metals, disinfectants, and clinically relevant antibiotics

Cerdeira, Louise; Monte, Daniel Farias Marinho do; Fuga, Bruna; Sellera, Fábio P.; Neves, Ingrith; Rodrigues, Liana Álvares; Landgraf, Mariza; Lincopán, Nilton

doi:10.1016/j.ygeno.2020.09.015

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…The CTX-M enzymes can be mostly clustered into five groups based on sequence homologies: CTX-M-1 (including CTX-M-15 and CTX-M-55), CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25 [ 91 ]. ESBLs of seafood-origin reported to date comprise CTX-M-1, CTX-M-8, CTX-M-9, CTX-M-14, CTX-M-15, CTX-M-24, CTX-M-27, CTX-M-55, CTX-M-65, CTX-M-130, SHV-5, SHV-12, SHV-32, SHV-186, SHV-187, TEM-24, TME-52 and OXA in E. coli , Salmonella or other Enterobacterales [ 38 , 39 , 42 , 44 , 45 , 46 , 64 , 76 , 89 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 ]. Additionally, class A PER-1 was identified from Vibrio parahaemolyticus of fish.…”

Section: Results (Headings As Per the Codex Guidelines)mentioning

confidence: 99%

Extended Spectrum β-Lactamase-Producing Enterobacterales of Shrimp and Salmon Available for Purchase by Consumers in Canada—A Risk Profile Using the Codex Framework

Uhland,

Li,

Mulvey

et al. 2023

Antibiotics

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The extended-spectrum β-lactamase (ESBL)-producing Enterobacterales (ESBL-EB) encompass several important human pathogens and are found on the World Health Organization (WHO) priority pathogens list of antibiotic-resistant bacteria. They are a group of organisms which demonstrate resistance to third-generation cephalosporins (3GC) and their presence has been documented worldwide, including in aquaculture and the aquatic environment. This risk profile was developed following the Codex Guidelines for Risk Analysis of Foodborne Antimicrobial Resistance with the objectives of describing the current state of knowledge of ESBL-EB in relation to retail shrimp and salmon available to consumers in Canada, the primary aquacultured species consumed in Canada. The risk profile found that Enterobacterales and ESBL-EB have been found in multiple aquatic environments, as well as multiple host species and production levels. Although the information available did not permit the conclusion as to whether there is a human health risk related to ESBLs in Enterobacterales in salmon and shrimp available for consumption by Canadians, ESBL-EB in imported seafood available at the retail level in Canada have been found. Surveillance activities to detect ESBL-EB in seafood are needed; salmon and shrimp could be used in initial surveillance activities, representing domestic and imported products.

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Section: Results (Headings As Per the Codex Guidelines)mentioning

confidence: 99%

Extended Spectrum β-Lactamase-Producing Enterobacterales of Shrimp and Salmon Available for Purchase by Consumers in Canada—A Risk Profile Using the Codex Framework

Uhland,

Li,

Mulvey

et al. 2023

Antibiotics

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“…However, the parrot was a companion animal [confirmed through written confirmation with the corresponding author ( 94 )] which implies a possible human-companion animal transmission. The qnrE1 gene was also identified in a MDR K. pneumoniae isolate from a native Amazonian fish in Brazil where it was harbored in a hybrid IncFIB/IncHI1B plasmid ( 95 ). qnrE1 originated from the chromosome of Enterobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Clinically relevant antibiotic resistance in Escherichia coli from black kites in southwestern Siberia: a genetic and phenotypic investigation

Tarabai

Krejčí

Karyakin³

et al. 2023

mSphere

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Wild birds including raptors can act as vectors of clinically relevant bacteria with antibiotic resistance. The aim of this study was to investigate the occurrence of antibiotic-resistant Escherichia coli in black kites ( Milvus migrans ) inhabiting localities in proximity to human-influenced environments in southwestern Siberia and investigate their virulence and plasmid contents. A total of 51 E. coli isolates mostly with multidrug resistance (MDR) profiles were obtained from cloacal swabs of 35 (64%, n = 55) kites. Genomic analyses of 36 whole genome sequenced E. coli isolates showed: (i) high prevalence and diversity of their antibiotic resistance genes (ARGs) and common association with ESBL/AmpC production (27/36, 75%), (ii) carriage of mcr-1 for colistin resistance on IncI2 plasmids in kites residing in proximity of two large cities, (iii) frequent association with class one integrase (IntI1, 22/36, 61%), and (iv) presence of sequence types (STs) linked to avian-pathogenic (APEC) and extra-intestinal pathogenic E. coli (ExPEC). Notably, numerous isolates had significant virulence content. One E. coli with APEC-associated ST354 carried qnrE1 encoding fluoroquinolone resistance on IncHI2-ST3 plasmid, the first detection of such a gene in E. coli from wildlife. Our results implicate black kites in southwestern Siberia as reservoirs for antibiotic-resistant E. coli . It also highlights the existing link between proximity of wildlife to human activities and their carriage of MDR bacteria including pathogenic STs with significant and clinically relevant antibiotic resistance determinants. IMPORTANCE Migratory birds have the potential to acquire and disperse clinically relevant antibiotic-resistant bacteria (ARB) and their associated antibiotic resistance genes (ARGs) through vast geographical regions. The opportunistic feeding behavior associated with some raptors including black kites and the growing anthropogenic influence on their natural habitats increase the transmission risk of multidrug resistance (MDR) and pathogenic bacteria from human and agricultural sources into the environment and wildlife. Thus, monitoring studies investigating antibiotic resistance in raptors may provide essential data that facilitate understanding the fate and evolution of ARB and ARGs in the environment and possible health risks for humans and animals associated with the acquisition of these resistance determinants by wildlife.

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“…A study by Obire and Guda (2002) [ 38 ] demonstrated the presence of species belonging to the genera Klebsiella , Citrobacter , Escherichia and Enterobacter in leachates from a landfill in Nigeria, which is consistent with the results found in this study. Although species belonging to these genera have been associated with fecal contamination [ 39 ], their presence in highly complex matrices such as leachates is [ 40 ] mainly associated with their ability to tolerate and bioremediate heavy-metal-contaminated environments [ 41 , 42 , 43 , 44 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Beta-Lactam-Resistant Enterobacterales Isolated from Landfill Leachates

et al. 2022

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Antibiotic resistance is one of the main challenges worldwide due to the high morbidity and mortality caused by infections produced by resistant bacteria. In Colombia, this problem has been studied mainly from the clinical perspective; however, it is scarcely studied in the leachates produced in landfills. The objective of this study was to detect, identify and determine the antibiotic sensitivity profile of Enterobacterales isolated from a leachate treatment plant located in Cali, Colombia. Detection was performed using selective culture media, bacterial identification using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF, bioMérieux) and by sequencing the gene coding for the 16S ribosomal RNA subunit when discrepancies were observed between phenotypic characteristics and MALDI-TOF. Antibiotic sensitivity profiling was determined using the automated VITEK®2 system (bioMérieux). Twenty-one isolates were obtained, of which Klebsiella pneumoniae was the most frequent (23.8%), and 34% of the isolates showed decreased sensitivity to beta-lactam antibiotics such as cefoxitin, ampicillin/sulbactam and piperacillin/tazobactam. These findings suggest that leachates from landfills could be a reservoir of pathogenic bacteria carrying antibiotic resistance determinants, so periodic microbiological characterization of these effluents should be performed, promoting the One Health approach.

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Genomic insights of Klebsiella pneumoniae isolated from a native Amazonian fish reveal wide resistome against heavy metals, disinfectants, and clinically relevant antibiotics

Cited by 16 publications

References 19 publications

Extended Spectrum β-Lactamase-Producing Enterobacterales of Shrimp and Salmon Available for Purchase by Consumers in Canada—A Risk Profile Using the Codex Framework

Extended Spectrum β-Lactamase-Producing Enterobacterales of Shrimp and Salmon Available for Purchase by Consumers in Canada—A Risk Profile Using the Codex Framework

Clinically relevant antibiotic resistance in Escherichia coli from black kites in southwestern Siberia: a genetic and phenotypic investigation

Beta-Lactam-Resistant Enterobacterales Isolated from Landfill Leachates

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