Genomic insights on heterogeneous resistance to vancomycin and teicoplanin in Methicillin-resistant Staphylococcus aureus: A first report from South India

Bakthavatchalam, Yamuna Devi; Babu, P G; Munusamy, Elakkiya; Dwarakanathan, Hariharan Triplicane; Rupali, Priscilla; Zervos, Marcus J.; Victor, Peter John; Veeraraghavan, Balaji

doi:10.1371/journal.pone.0227009

Cited by 30 publications

(24 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, in the present study, all the hVISH isolates had dual mutations D471E with I527M in the rpo B gene. Interestingly, rpoB mutation-driven vancomycin heteroresistance has been reported in S. aureus [ 34 , 35 ], S. epidermidis [ 16 ] and S. capitis [ 7 ]. It is interesting to note that the occurrence of dual rpo B mutations (D471E and I527M) contributes to rifampicin resistance in S. haemolyticus .…”

Section: Discussionmentioning

confidence: 99%

Vancomycin Heteroresistance in Staphylococcus Haemolyticus : Elusive Phenotype

et al. 2021

Self Cite

View full text Add to dashboard Cite

Aim: To determine the presence of vancomycin heteroresistance in Staphylococcus haemolyticus. Materials & methods: A total of 48 rifampicin-resistant S. haemolyticus isolates from bloodstream infections were included. Vancomycin heteroresistance was determined using the population analysis profile-area under curve (PAP-AUC) method. All the isolates were screened for the presence of mecA gene, mutations in the rpoB gene, staphylococcal cassette chromosome mec and multilocus sequence types. Results: Fifteen isolates were identified as heteroresistant vancomycin-intermediate S. haemolyticus using PAP-AUC method. Dual rpoB mutations (D471E and I527M) contributed for the rifampicin resistance. The sequence types of heteroresistant vancomycin-intermediate S. haemolyticus were highly diverse. Conclusion: These findings illustrate the potential of S. haemolyticus to develop heteroresistance, which emphasizes the need for routine surveillance of S. haemolyticus isolated from intensive care units for infection control practices.

show abstract

Section: Discussionmentioning

confidence: 99%

Vancomycin Heteroresistance in Staphylococcus Haemolyticus : Elusive Phenotype

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…These heterogeneous populations represent a clinical challenge since they can lead to treatment failure. Sometimes, cross-resistance between teicoplanin and vancomycin can be observed [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture

et al. 2020

View full text Add to dashboard Cite

The main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and agr typing methods. The majority of S. aureus from human sources were resistant to cefoxitin (and harbor the mecA gene) and fluoroquinolones, whereas only four strains of S. aureus from animal sources revealed resistance to ciprofloxacin. In the set of S. aureus isolated from humans, the most frequent spa, MLST, and agr group were t032, ST22, and I, respectively. In strains from animal origin the most common spa, MLST, and agr group found were t2383, ST398, and III/not typable, respectively. S. aureus from humans and animals were identified either in clonal complexes CC5, CC30, and CC398, suggesting that they have the same putative founder in their evolution. Considering the three CCs encompassing strains from human and animal reservoirs with different spa-types, we can hypothesize that this might reflect an adaptation to different phylogenetic lineages in those reservoirs (host species) probably associated to genetic diversification of pre-existing strains.

show abstract

“…The glycopeptide family of antibiotics, to which teicoplanin also belongs, remains a mainstay option for suspected severe SSTI and invasive MRSA infections [3], even though there are multiple alternatives, and it has a potential for renal toxicity, Unfortunately, extensive antibiotic use in healthcare and agriculture in recent years [4] has determined a selection pressure on bacteria leading to a strong increase [5,6] in the emergence of MRSA isolates with reduced susceptibility to teicoplanin [7].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Cathelicidin LL-37 in an MRSA Wound Infection Mouse Model

et al. 2021

View full text Add to dashboard Cite

Background: LL-37 is the only human antimicrobial peptide that belongs to the cathelicidins. The aim of the study was to evaluate the efficacy of LL-37 in the management of MRSA-infected surgical wounds in mice. Methods: A wound on the back of adult male BALB/c mice was made and inoculated with Staphylococcus aureus. Two control groups were formed (uninfected and not treated, C0; infected and not treated, C1) and six contaminated groups were treated, respectively, with: teicoplanin, LL-37, given topically and /or systemically. Histological examination of VEGF expression and micro-vessel density, and bacterial cultures of wound tissues, were performed. Results: Histological examination of wounds in the group treated with topical and intraperitoneal LL-37 showed increased re-epithelialization, formation of the granulation tissue, collagen organization, and angiogenesis. Conclusions: Based on the mode of action, LL-37 has a potential future role in the management of infected wounds.

show abstract

Genomic insights on heterogeneous resistance to vancomycin and teicoplanin in Methicillin-resistant Staphylococcus aureus: A first report from South India

Cited by 30 publications

References 71 publications

Vancomycin Heteroresistance in Staphylococcus Haemolyticus : Elusive Phenotype

Vancomycin Heteroresistance in Staphylococcus Haemolyticus : Elusive Phenotype

Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture

Efficacy of Cathelicidin LL-37 in an MRSA Wound Infection Mouse Model

Contact Info

Product

Resources

About