Genomic organization and assignment of the interleukin 7 gene (IL7) to porcine chromosome 4q11→q13 by FISH and by analysis of radiation hybrid panels

Uenishi, Hirohide; Hiraiwa, Hideki; Sawazaki, T.; Kiuchi, Sachiko; Yasue, Hiroshi

doi:10.1159/000056951

Cited by 9 publications

(4 citation statements)

References 49 publications

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“…Porcine IL2 has 82.3% (70.7%), porcine IFN␥ has 75.1% (60.2%), porcine IL12 (p40) has 86.3% (82.9%), and porcine IL7 has 85% (73%) nucleotide sequence (amino acid sequence) homologies with their human counterparts, respectively (170,249). Porcine IL6 shares a significant homology with human IL6.…”

Section: Cytokines and Chemokinesmentioning

confidence: 99%

The porcine lung as a potential model for cystic fibrosis

Rogers¹,

Abraham²,

Brogden³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

224

219

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Airway disease currently causes most of the morbidity and mortality in patients with cystic fibrosis (CF). However, understanding the pathogenesis of CF lung disease and developing novel therapeutic strategies have been hampered by the limitations of current models. Although the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) has been targeted in mice, CF mice fail to develop lung or pancreatic disease like that in humans. In many respects, the anatomy, biochemistry, physiology, size, and genetics of pigs resemble those of humans. Thus pigs with a targeted CFTR gene might provide a good model for CF. Here, we review aspects of porcine airways and lung that are relevant to CF.

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Section: Cytokines and Chemokinesmentioning

confidence: 99%

The porcine lung as a potential model for cystic fibrosis

Rogers¹,

Abraham²,

Brogden³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

224

219

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“…The chromosome spreads were then covered with the mixture and incubated at 37°C for 16 hr in a humidified chamber. Hybridization signals were detected using an FITC-conjugated streptavidin/biotinylated anti-streptavidin system (Vector Laboratories, Burlingame, CA, U.S.A.), and located on the specific chromosome according to the procedure described previously [12,28]. The hybridization signals visualized under B2 excitation were captured by a cooled CCD camera (Hamam atsu Ph oton ics Inc., Hamamatsu, Japan), and the R-band patterns under G excitation were superimposed on the image of the hybridization signals.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Molecular Cloning, Chromosomal Location, and Biological Activity of Porcine Interleukin-21

Muneta

Kikuma

Uenishi

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 µg/ml concanavalin A (ConA), 10 µg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 µg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identi ty to the bovine, human, and murine IL-21, respectively. The porcine IL-21 gene was mapped to porcine chromosome 8 (8q22→q23) by means of fluorescence in situ hybridization and radiation hybrid mapping, where the porcine IL-2 gene had been mapped nearby. The recombinant porcine mature IL-21 expressed by E. coli induced dose-dependent proliferation and IFN-γ production from a human NK cell line, NK0. The porcine IL-21 identified in this study will be helpful for the enhancement of innate immune responses of pigs. KEY WORDS: cloning, innate immunity, interleukin-21, NK cell, porcine.J. Vet. Med. Sci. 66(3): 269-275, 2004 Interleukin-21 (IL-21) is a novel cytokine that regulates the proliferation of T and B cells, and involves the maturation and expansion of NK cells from bone marrow progenitor cells [20]. IL-21 is produced by activated T cells [20,21], and is a four-helix-bundle type I cytokine with structural similarity to IL-2, 21]. IL-21 receptor also has significant amino acid similarities with IL-2, , and shares the common γ chain for the subunit of the IL-21 receptor complex [2]. The common γ chain-containing receptor binded by its ligand such as IL-2 and IL-21 causes the activation of the Janus tyrosine kinase family 3 (JAK3) [6,17], and transduce its signal to the downstream molecules. Recent studies have indicated that IL-21 has various biological activities that affect T and NK cell functions [20,21] . Currently, IL-21 is thought to be a mediator cytokine, which promotes the transition from innate to adaptive immunity [9,21].One of the most interesting characteristics of IL-21 is its effect on NK cell maturation and expansion [20], because pigs have high NK cell populations in the peripheral blood [31], and many piglets suffer from diarrhea and respiratory diseases in their neonatal periods during which adaptive immunity is immature. We previously reported the cloning and expression of porcine , and the IFN-γ induction by IL-18 from neonatal piglets [13]. Recently, Strengell et al. reported that IL-21 also enhances IFN-γ production from human NK and T cells in synergy with . Taken, together, we would like to isolate porcine IL-21, a recent cytokine that promotes the transition from innate to adaptive immunity [9,21], in order to utilize this cytokine for the enhancement of neonatal immunity of pigs. We also reported the cloning, and the expression of bovine IL-21 using a baculovirus expression system [15].In the present study, cDNA encoding porcine IL-21 was cloned and characterized, and the chromos...

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“…FISH was performed as described previously (Uenishi et al, 2001) with a slight modification. DNA of the BAC clone 647B12, containing the swine CCR7 gene, was biotinylated with a biotin nick-translation labeling mix kit (Roche Diagnostics, Mannheim, Germany), while the BAC clone 390D11 containing the swine MYH4 gene was labeled with a DIG nick-translation labeling mix kit (Roche Diagnostics).…”

Section: Mapping By Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Molecular cloning and chromosomal assignment to SSC12p13→p11 of swine chemokine receptor CCR7

Shinkai¹,

Muneta

Eguchi

et al. 2003

Cytogenet Genome Res

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We cloned a gene encoding the swine chemokine (C-C motif) receptor 7 (CCR7) and clarified its genomic structure and chromosomal assignment. The ORF and deduced amino-acid sequence were highly conserved with human and mouse CCR7. The swine CCR7 gene was mapped to SSC12p13→p11 by FISH analysis. Stimulation of swine peripheral blood mononuclear cells by IL-12 and IL-18, considered potent inducers of Th1 cells from analyses in humans and mice, downregulated the expression of CCR7. This is the first report of the molecular cloning, chromosomal assignment and characterization of a chemokine receptor in swine.

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Genomic organization and assignment of the interleukin 7 gene (IL7) to porcine chromosome 4q11→q13 by FISH and by analysis of radiation hybrid panels

Cited by 9 publications

References 49 publications

The porcine lung as a potential model for cystic fibrosis

The porcine lung as a potential model for cystic fibrosis

Molecular Cloning, Chromosomal Location, and Biological Activity of Porcine Interleukin-21

Molecular cloning and chromosomal assignment to SSC12p13→p11 of swine chemokine receptor CCR7

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