Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis

Abstract

In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-responsive element. Promoter deletion analysis using a luciferase reporter gene identified regulatory elements. Challenge with white spot syndrome virus increased expression from the promoter-deletion constructs. These results suggest that FcTR might play an important role in the shrimp immune response.

Reference genes for gene expression studies in the mouse heart

“…The changing contributions of different cell types in different cardiac compartments during developmental stages and pathologies make that either a large set of reference genes is required or that different sets of at least two reference genes are required for different experimental and clinical set-ups. 6,14 .…”

“…Several papers report on reference genes for heart infarction and hypertrophy models 6,14,15 . However, these studies were all restricted to two tissue types; tissue from sham operated mice compared to a single stage after treatment.…”

“…Potential reference genes were selected based on literature 6,14,15 . Nine candidate genes were selected to match the requirement for the analysis of reference gene stability 4 .…”