Genomic Organization, Chromosomal Localization, and Expression of the Murine RAB3D Gene

Adachi, Roberto; Nigam, Rupesh; Tuvim, Michael J.; DeMayo, Francesco J.; Dickey, Burton F.

doi:10.1006/bbrc.2000.3032

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…Lung tissue has been identified as a major source of mRNA encoding the small GTP-binding protein rab3D (21). In contrast, a recent study employing quantitative immunoblotting to map the tissue distribution of rab3 isoforms failed to detect rab3D protein in Triton X-114 extracts of lung tissue (22).…”

Section: Discussionmentioning

confidence: 99%

Rab3D and Actin Reveal Distinct Lamellar Body Subpopulations in Alveolar Epithelial Type II Cells

Weeren

Graaff

Jamieson

et al. 2004

Am J Respir Cell Mol Biol

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Rab3D is a small GTP-binding protein associated with secretory vesicles in various exocrine and endocrine cells, where it has been implicated in regulated exocytosis. Data obtained previously in pancreas have suggested that rab3D is involved in the coating of secretory granules with filamentous actin. In the present study we employed Western blot analysis, immunofluorescence, and immunoelectron microscopy to examine the distribution of rab3D in rat lung. Rab3D immunoreactivity was detected in bronchiolar Clara cells and alveolar epithelial type II (AET-II) cells. In both cell types, rab3D displayed preferential localization to secretory vesicles that were identified using specific antibodies against Clara Cell Secretory Protein and p180 lamellar body protein, respectively. Interestingly, rab3D was associated with only 24% of the lamellar bodies in AET-II cells. Rab3D-positive lamellar bodies were typically in close proximity of the apical plasma membrane, where exocytosis occurs. Another subpopulation of lamellar bodies, constituting only 2%, was not only rab3D-positive but could also be labeled with the filamentous-actin probe phalloidin. A third subpopulation, constituting 9%, displayed actin coating without rab3D staining. We propose that these three lamellar body subpopulations represent consecutive intermediates along the regulated exocytotic pathway, implying that rab3D release and actin coating are intimately linked processes.

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Section: Discussionmentioning

confidence: 99%

Rab3D and Actin Reveal Distinct Lamellar Body Subpopulations in Alveolar Epithelial Type II Cells

Weeren

Graaff

Jamieson

et al. 2004

Am J Respir Cell Mol Biol

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“…This fragment was first cloned into pCRII-TOPO Blunt vector and then subcloned into a firefly luciferase reporter vector (pGL3 Basic; Promega, Madison, WI) using the HindIII and XhoI sites in the PCRII and pGL3 multicloning sites. A 950 bp fragment of the Rab3D 5Ј untranslated region was generated by PCR and subcloning from a genomic DNA fragment reported previously by our laboratory (30). Primer sequences were 5Ј-GGTACCCGACAGAGGGAAACATTG-AGG (sense) and 5Ј-CTCGAGCCGCACAGGGCTGCGACCCTCG (antisense), and include restriction endonuclease sites (bold) for subcloning into KpnI (sense) and XhoI (antisense) sites in pGL3 Basic.…”

Section: Muc5ac Rab3d and Ccsp Promoter Cloning And Analysismentioning

confidence: 99%

Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen-Challenged Mice

Evans

Williams

Tuvim

et al. 2004

Am J Respir Cell Mol Biol

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344

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Airway mucus hypersecretion is a prominent feature of many obstructive lung diseases. We thus determined the ontogeny and exocytic phenotype of mouse airway mucous cells. In naive mice, ciliated (approximately 40%) and nonciliated (approximately 60%) epithelial cells line the airways, and > 95% of the nonciliated cells are Clara cells that contain Clara cell secretory protein (CCSP). Mucous cells comprise < 5% of the nonciliated cells. After sensitization and a single aerosol antigen challenge, alcian blue-periodic acid Schiff's positive mucous cell numbers increase dramatically, appearing 6 h after challenge (21% of nonciliated/nonbasal cells), peaking from Days 1-7 (99%), and persisting at Day 28 (65%). Throughout the induction and resolution of mucous metaplasia, ciliated and Clara cell numbers identified immunohistochemically change only slightly. Intracellular mucin content peaks at Day 7, and mucin expression is limited specifically to a Clara cell subset in airway generations 2-4 that continue to express CCSP. Functionally, Clara cells are secretory cells that express the regulated exocytic marker Rab3D and, in antigen-challenged mice, rapidly secrete mucin in response to inhaled ATP in a dose-dependent manner. Thus, Clara cells show great plasticity in structure and secretory products, yet have molecular and functional continuity in their identity as specialized apical secretory cells.

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“…Intron/exon distribution is maintained depending on the subfamily to which Rab GT-Pases belong, thus maintaining a conserved gene structure. It has been reported that within the Rab GTPase families, there is a tendency to maintain this intron-exon distribution related to a specific function within the same family [40,47,48]. An example is observed in the Populus trichocarpa Rab GTPase family, whereas the intron-exon distribution remains conserved within each subfamily (RabA-RabH) [42].…”

Section: Discussionmentioning

confidence: 99%

Identification, Classification, and Transcriptional Analysis of Rab GTPase Genes from Tomato (Solanum lycopersicum) Reveals Salt Stress Response Genes

Soto,

San Martín-Davison,

Salinas-Cornejo

et al. 2024

Genes

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Salinity in plants generates an osmotic and ionic imbalance inside cells that compromises the viability of the plant. Rab GTPases, the largest family within the small GTPase superfamily, play pivotal roles as regulators of vesicular trafficking in plants, including the economically important and globally cultivated tomato (Solanum lycopersicum). Despite their significance, the specific involvement of these small GTPases in tomato vesicular trafficking and their role under saline stress remains poorly understood. In this work, we identified and classified 54 genes encoding Rab GTPases in cultivated tomato, elucidating their genomic distribution and structural characteristics. We conducted an analysis of duplication events within the S. lycopersicum genome, as well as an examination of gene structure and conserved motifs. In addition, we investigated the transcriptional profiles for these Rab GTPases in various tissues of cultivated and wild tomato species using microarray-based analysis. The results showed predominantly low expression in most of the genes in both leaves and vegetative meristem, contrasting with notably high expression levels observed in seedling roots. Also, a greater increase in gene expression in shoots from salt-tolerant wild tomato species was observed under normal conditions when comparing Solanum habrochaites, Solanum pennellii, and Solanum pimpinellifolium with S. lycopersicum. Furthermore, an expression analysis of Rab GTPases from Solanum chilense in leaves and roots under salt stress treatment were also carried out for their characterization. These findings revealed that specific Rab GTPases from the endocytic pathway and the trans-Golgi network (TGN) showed higher induction in plants exposed to saline stress conditions. Likewise, disparities in gene expression were observed both among members of the same Rab GTPase subfamily and between different subfamilies. Overall, this work emphasizes the high degree of conservation of Rab GTPases, their high functional diversification in higher plants, and the essential role in mediating salt stress tolerance and suggests their potential for further exploration of vesicular trafficking mechanisms in response to abiotic stress conditions.

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Genomic Organization, Chromosomal Localization, and Expression of the Murine RAB3D Gene

Cited by 8 publications

References 54 publications

Rab3D and Actin Reveal Distinct Lamellar Body Subpopulations in Alveolar Epithelial Type II Cells

Rab3D and Actin Reveal Distinct Lamellar Body Subpopulations in Alveolar Epithelial Type II Cells

Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen-Challenged Mice

Identification, Classification, and Transcriptional Analysis of Rab GTPase Genes from Tomato (Solanum lycopersicum) Reveals Salt Stress Response Genes

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