Genomic organization of major sperm protein genes and pseudogenes in the nematode Caenorhabditis elegans

Ward, Samuel; Burke, Daniel J.; Sulston, John; Coulson, Alan; Albertson, Donna G.; Ammons, David; Klass, Michael; Hogan, Eileen

doi:10.1016/0022-2836(88)90374-9

Cited by 66 publications

(38 citation statements)

References 41 publications

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“…Until now, MSP orthologues have been found only within nematodes. In these, the protein is exclusively expressed in testicular tissues, but contributes to 15-17% of the total and 40% of the soluble protein in nematode sperm (Klass and Hirsh 1981;Nelson and Ward 1981;Ward et al 1988). MSPs have a molecular weight of 14-15 kDa, an isoelectric point of about 8.0, and exist in solution as an extremely stable symmetrical dimer (Klass and Hirsh 1981;King et al 1992;Haaf et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins

2008

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Major sperm proteins (MSPs) represent a protein family occurring in nematodes only. Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequence tracts consistent with the Kozak and polyadenylation consensus sequence. On genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. Conceptual translation inferred two MSP isoforms due to one substitution within the 126-amino acid polypeptide. Bioinformatic analysis predicted that bovine lungworm MSP folds into an immunoglobulin-like seven-stranded beta sandwich as known for Caenorhabditis elegans and Ascaris suum. Furthermore, bovine lungworm MSP is confidentially predicted to be N-terminal-acetylated and secreted via a non-classical pathway. Quantitative real-time polymerase chain reaction analysis using ten developmental lungworm stages showed that msp is transcribed mainly in adult male parasites and in some degree in hypobiotic L5. However, marginal msp transcription was detectable in all of the investigated developmental lungworm stages.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins

2008

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“…To identify a down-regulated target gene that was responsible for the fertilization defect of the ife-1 mutants, we examined whether any of these genes showed the same phenotype as ife-1 mutants when depleted by RNAi (Table 3). Since the major sperm proteins (MSPs), which were one of the 4 down-regulated proteins, are encoded by a family of over 50 almost identical genes called msp genes (Ward et al, 1988), we tested some of the msp genes for RNAi. We found that RNAi for gln-2, lbp-9, and four msp genes did not cause significant sterility (Table 3, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…This family consists of closely related, small, basic proteins that make up 15% of sperm protein (Ward et al, 1988). They are encoded by a multigene family of over 50 genes, including many pseudogenes (Ward et al, 1988). At the transcriptional level, at least some of the msp genes seem to be regulated by ELT-1, which is a germline-enriched GATA transcription factor (Shim, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Regulation of Sperm-Specific Proteins by IFE-1, a Germline-Specific Homolog of eIF4E, in C. elegans

Kawasaki

Jeong

Shim

2011

Molecules and Cells

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IFE-1 is one of the five C. elegans homologs of eIF4E, which is the mRNA 5′ cap-binding component of the translation initiation complex eIF4F. Depletion of IFE-1 causes defects in sperm, suggesting that IFE-1 regulates a subset of genes required for sperm functions. To further understand the molecular function of IFE-1, proteomic analysis was performed to search for sperm proteins that are downregulated in ife-1(ok1978); fem-3(q20) mutants relative to the fem-3(q20) control. The fem-3(q20) mutant background was used because it only produces sperm at restrictive temperature. Total worm proteins were subjected to 2D-DIGE, and differentially expressed protein spots were further identified by MALDI-TOF mass spectrometry. Among the identified proteins, GSP-3 and Major Sperm Proteins (MSPs) were found to be significantly down-regulated in the ife-1(ok1978) mutant. Moreover, RNAi of gsp-3 caused an ife-1-like phenotype. These results suggest that IFE-1 is required for efficient expression of some sperm-specific proteins, and the fertilization defect of ife-1 mutant is caused mainly by a reduced level of GSP-3.

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“…MSP proteins are encoded by a multigene family, which precludes the ability to obtain a genetic mutant easily (Ward et al 1988). MSP isoforms differ by only one to four amino acids.…”

Section: Msp a Protein With Cytoskeletal And Signaling Propertiesmentioning

confidence: 99%

The multifacetedC. elegansmajor sperm protein: an ephrin signaling antagonist in oocyte maturation: Figure 1.

Kuwabara¹

2003

Genes Dev.

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Genomic organization of major sperm protein genes and pseudogenes in the nematode Caenorhabditis elegans

Cited by 66 publications

References 41 publications

Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins

Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins

Regulation of Sperm-Specific Proteins by IFE-1, a Germline-Specific Homolog of eIF4E, in C. elegans

The multifacetedC. elegansmajor sperm protein: an ephrin signaling antagonist in oocyte maturation: Figure 1.

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