Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains The GenBank accession numbers for the sequences determined in this work are given in Table 4 T4 .

Mora, Diego; Fortina, María Grazia; Parini, Carlo; Daffonchio, D.; Manachini, P. L.

doi:10.1099/00221287-146-8-2027

Cited by 84 publications

(39 citation statements)

References 37 publications

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“…4A, lanes 4 to 7) sharing DNA fragments of approximately 80, 130, and 170 kb. Therefore, both approaches suggest that the species can be divided into at least two genotypic groups and this diversity within the species is in agreement with recent studies (29,30). Interestingly, two P. pentosaceus strains, NCIMB 8127 and NCIMB 8952, which shared the P. acidilactici PFGE patterns for NotI (Fig.…”

Section: Vol 68 2002 Rapd Pcr and Pfge Analysis Of Pediococci 767supporting

confidence: 75%

“…To date, RAPD PCR studies of pediococci have been based on single random primers. An 18-mer primer originally designed to target a pediocin-encoding gene on a plasmid from P. acidilactici was only effective at discriminating strains of P. acidilactici, with 19 strains producing seven distinct RAPD patterns (29,30). In another study, a 17-mer primer discriminated nine pediococci isolated from the gastrointestinal tracts of ducks into four groups and partial 16S ribosomal sequencing indicated that each group represented a single species (21).…”

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confidence: 99%

“…These include the use of specific DNA target probes (23,27,28,29,37), ribotyping (2,18,38), total DNA-DNA hybridization (1,13), 16S rRNA gene sequencing (2,10,21,33), randomly amplified polymorphic DNA (RAPD) PCR (21,29,30,32) and pulsed-field gel electrophoresis (PFGE) (3,12,24).…”

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confidence: 99%

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Genomic Diversity within the Genus Pediococcus as Revealed by Randomly Amplified Polymorphic DNA PCR and Pulsed-Field Gel Electrophoresis

Simpson

Stanton

Fitzgerald

et al. 2002

Appl Environ Microbiol

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The genomic diversity of 33 previously assigned strains from six species within the genus Pediococcus was assessed by randomly amplified polymorphic DNA (RAPD) PCR and pulsed-field-gel electrophoresis (PFGE). The RAPD PCR patterns produced by two separate random primers, termed P1 (ACGCGCCCT) and P2 (ATGTAACGCC), were compared by the Pearson correlation coefficient and the unweighted pair group method with arithmetic averages clustering algorithm. Pattern variations between repeat samples set a strain discrimination threshold of less than 70% similarity. P1 and P2 primers alone and in combination produced 14, 21, and 28 distinct patterns, respectively. When each strain was assigned with a type strain with which it shared the highest level of similarity, both primers grouped 17 of the 27 strains to their proposed species. PFGE following genomic digestion with the restriction enzymes ApaI, NotI, and AscI produced 30, 32, and 28 distinct macrorestriction patterns, respectively. Specific DNA fragments within the NotI and AscI macrorestriction patterns for each strain were observed that allowed 27 of the 33 strains to be assigned to their proposed species. For example, following digestion with AscI, all Pediococcus parvulus strains were characterized by two DNA fragments, one of approximately 220 kb and another between 700 and 800 kb. The exceptions correlated with those observed with both RAPD PCR primers and included three P. damnosus and two P. pentosaceus strains that grew at temperatures regarded as nonpermissive for their proposed species but not for those with which they grouped.

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Section: Vol 68 2002 Rapd Pcr and Pfge Analysis Of Pediococci 767supporting

confidence: 75%

mentioning

confidence: 99%

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Genomic Diversity within the Genus Pediococcus as Revealed by Randomly Amplified Polymorphic DNA PCR and Pulsed-Field Gel Electrophoresis

Simpson

Stanton

Fitzgerald

et al. 2002

Appl Environ Microbiol

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“…New techniques could be used to analyze such variants, such as multilocus typing, which analyzes several housekeeping genes involved in transcriptional or translational cell machinery, to highlight a high degree of genetic polymorphisms among strains of a species (22). In this study, the growth characteristics of the two variants in different types of milk and during yogurt production were indistinguishable from those of the wild-type strain (data not shown).…”

Section: Discussionmentioning

confidence: 70%

Two-Dimensional Electrophoresis Study of Lactobacillus delbrueckii subsp. bulgaricus Thermotolerance

Gouesbet

Jan

Boyaval

2002

Appl Environ Microbiol

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The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced viability, observed after 10 min at 65°C, resulted from the overexpression of HSP and from mechanisms not linked to protein synthesis. In order to analyze the thermoadaptation mechanisms involved, thermoresistant variants were selected. These variants showed enhanced constitutive tolerance toward heat shock. However, contrary to the wild-type strain, these variants were poorly protected after osmotic or heat pretreatments. This result suggests that above a certain threshold, cells reach a maximum level of protection that cannot be easily exceeded. A comparison of protein patterns showed that the variants were able to induce more rapidly their adaptive mechanisms than the original strain. In particular, the variants were able to express constitutively more HSP, leading to the higher level of thermoprotection observed. This is the first report of the study by 2-DE of the heat stress response in L. delbrueckii subsp. bulgaricus.Lactobacillus delbrueckii subsp. bulgaricus is one of the most important industrial lactic acid bacteria. This fact is mainly due to its use in yogurt and fermented milk for human consumption. However, the price of current commercial preparations restricts its use, mainly because of the high susceptibility of L. delbrueckii subsp. bulgaricus to preservation processes. For example, this species would be a very interesting probiotic (40) if the production cost of these sensitive bacteria could be decreased substantially. This goal could be achieved by increasing cell survival or by using a less deleterious production process. Direct spray drying of vegetative microbial cells is emerging (3) as an efficient and inexpensive preservation method with a high output and may lower this cost. Knowledge of the resistance of these bacteria to heat stress would be helpful for mastering cell viability during this dehydration treatment. Teixeira et al. (35) studied L. delbrueckii subsp. bulgaricus thermotolerance and clearly showed the successful application of such a preservation method to concentrated suspensions of these bacteria (34,35). When cells were submitted to a heat pretreatment at 50°C or to a hyperosmotic pretreatment before lethal temperature challenge (65°C), the viability of the cells increased (13). Nevertheless, little is known of potential methods for enhancing cell survival and mechanisms used by the cell to protect itself from heat stress or to repair damage. Two-dimensional electrophoresis (2-DE) of proteins is currently one of the highest-resolution analytical techniques available for the study of protein expression patterns (the promising genome-wide DNA arrays are not yet available for L. delbrueckii subsp. bulgaricus). New technological developments have improved r...

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“…1). Identification to species level is best obtained by 16S rDNA gene sequencing Kurzak et al, 1998;Omar et al, 2000;Barney et al, 2001), species-specific DNA probes (Lonvaud-Funel et al, 1993;Mora et al, 1997;Rodrigues et al, 1997), ribotyping (Jager & Harlander, 1992;Satokari et al, 2000;Barney et al, 2001;Santos et al, 2005), randomly amplified polymorphic DNA (RAPD) PCR (Kurzak et al, 1998;Nigatu et al, 1998;Mora et al, 2000;Simpson et al, 2002;Fujii et al, 2005;Tamang et al, 2005), and pulsed-field gel electrophoresis (PFGE) ( Dellaglio et al, 1981;Luchansky et al, 1992;Barros et al, 2001). Simpson et al (2006) developed a rapid PFGE protocol for species-and strain-level differentiation.…”

Section: The Genus Pediococcusmentioning

confidence: 99%

Taxonomic Status of Lactic Acid Bacteria in Wine and Key Characteristics to Differentiate Species

Dicks

Endo

2016

SAJEV

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Oenococcus oeni is the best malolactic bacterium adapted to low pH and the high SO 2 and ethanol concentrations in wine. Leuconostoc mesenteroides and Leuconostoc paramesenteroides (now classified as Weissella paramesenteroides) have also been isolated from wine. Pediococcus damnosus is not often found in wine and is considered a contaminant of high pH wines. Pediococcus inopinatus, Pediococcus parvulus and Pediococcus pentosaceus have occasionally been isolated from wines. Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus hilgardii (previously Lactobacillus vermiforme), Lactobacillus fructivorans (previously Lactobacillus trichoides and Lactobacillus heterohiochii) and Lactobacillus fermentum have been isolated from most wines. Lactobacillus hilgardii and L. fructivorans are resistant to high acid and alcohol and have been isolated from spoiled fortified wines. Lactobacillus vini, Lactobacillus lindneri, Lactobacillus nagelii and Lactobacillus kunkeei have been described more recently. The latter two species are known to cause sluggish or stuck alcoholic fermentations in wine. Although Lactobacillus collinoides and Lactobacillus mali (previously Lactobacillus yamanashiensis) decarboxylate L-malic acid, they are more often found in cider and fruit juices. Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus diolivorans, Lactobacillus jensenii and Lactobacillus paracasei are seldomly isolated from wines. Some strains of Lactobacillus casei may be closer related to Lactobacillus paracasei or a distant relative, Lactobacillus zeae. Oenococcus kitaharae, isolated from compost is genetically closely related to Oenococcus oeni, but does not decarboxylate malate, prefers higher growth pH and is phenotypically well distinguished from O. oeni. This review summarises the current taxonomic status of malolactic bacteria and lists key phenotypic characteristics that may be used to identify the species.

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Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains The GenBank accession numbers for the sequences determined in this work are given in Table 4 T4 .

Cited by 84 publications

References 37 publications

Genomic Diversity within the Genus Pediococcus as Revealed by Randomly Amplified Polymorphic DNA PCR and Pulsed-Field Gel Electrophoresis

Genomic Diversity within the Genus Pediococcus as Revealed by Randomly Amplified Polymorphic DNA PCR and Pulsed-Field Gel Electrophoresis

Two-Dimensional Electrophoresis Study of Lactobacillus delbrueckii subsp. bulgaricus Thermotolerance

Taxonomic Status of Lactic Acid Bacteria in Wine and Key Characteristics to Differentiate Species

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