Genomics analysis of KPC-2 and NDM-5-producing Enterobacteriaceae in migratory birds from Qinghai Lake, China

Bai, Shuancheng; Fang, Liangxing; Xiao, Hongliang; Zhang, Yin; Guo, Wenying; Zhang, Jixing; Liu, Juan; Zhang, Yan; Wang, Minge; Sun, Ruanyang; Han, Lu; Yu, Yang; Sun, Jian; Liu, Yahong; Liao, Xiaoping

doi:10.1007/s00253-023-12746-3

Cited by 2 publications

(1 citation statement)

References 53 publications

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“…2 The Klebsiella pneumoniae species complex (KpSC) comprises the main disseminators of carbapenemases from health-care institutions to other sources. 3 In this respect, carbapenemase-positive K. pneumoniae strains have been reported in migratory birds, 4 companion animals, 5 aquatic ecosystems, 6 soils, 7 and vegetables, 8 and may have been amplified by the COVID-19 pandemic. 9,10 Indeed, the coexistence of carbapenemases in Enterobacterales has emerged rapidly, causing pan-⊎-lactam resistance.…”

Section: Introductionmentioning

confidence: 99%

Emergence of carbapenem‐resistant Klebsiella pneumoniae species complex from agrifood systems: detection of ST6326 co‐producing KPC‐2 and NDM‐1

Furlan,

da Silva Rosa,

Ramos

et al. 2024

J Sci Food Agric

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BackgroundKlebsiella pneumoniae species complex (KpSC) is an important disseminator of carbapenemase‐encoding genes, mainly blaKPC‐2 and blaNDM‐1, from hospitals to the environment. Consequently, carbapenem‐resistant strains can be spread through the agrifood system, raising concerns about food safety. Therefore, this study aimed to isolate carbapenem‐resistant KpSC strains from agricultural and environmental sectors and characterize them by phenotypic, molecular, and genomic analyses.ResultsK. pneumoniae and Klebsiella quasipneumoniae strains isolated from soils with lemon, guava, and fig cultivation, and surface waters displayed an extensive drug resistance profile and carried blaKPC‐2, blaNDM‐1, or both. In addition to carbapenemase‐encoding genes, KpSC strains harbor a broad resistome (antimicrobial resistance and metal tolerance) and present putative hypervirulence. Soil‐derived K. pneumoniae strains were assigned as high‐risk clones (ST11 and ST307) and harbored the blaKPC‐2 gene associated with Tn4401b and Tn3‐like elements on IncN‐pST15 and IncX5 plasmids. In surface waters, the coexistence of blaKPC‐2 and blaNDM‐1 genes was identified in the K. pneumoniae ST6326, a new carbapenem‐resistant regional Brazilian clone. In this case, blaKPC‐2 with Tn4401a isoform and blaNDM‐1 associated with a Tn125‐like transposon were located on different plasmids. In addition, K. quasipneumoniae ST526 presented the blaNDM‐1 gene associated with a Tn3000 transposon on an IncX3 plasmid.ConclusionThese findings alert for the transmission of carbapenemase‐positive KpSC across the agricultural and environmental sectors, raising critical food safety and environmental issues.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

Emergence of carbapenem‐resistant Klebsiella pneumoniae species complex from agrifood systems: detection of ST6326 co‐producing KPC‐2 and NDM‐1

Furlan,

da Silva Rosa,

Ramos

et al. 2024

J Sci Food Agric

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Decoding the enigma: unveiling the transmission characteristics of waterfowl-associated blaNDM-5-positive Escherichia coli in select regions of China

Zhang,

Shu,

Yang

et al. 2024

Front. Microbiol.

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Escherichia coli (E. coli) serves as a critical indicator microorganism for assessing the prevalence and dissemination of antibiotic resistance, notably harboring various antibiotic-resistant genes (ARGs). Among these, the emergence of the blaNDM gene represents a significant threat to public health, especially since carbapenem antibiotics are vital for treating severe infections caused by Gram-negative bacteria. This study aimed to characterize the antibiotic resistance features of blaNDM-5-positive E. coli strains isolated from waterfowl in several regions of China and elucidate the dissemination patterns of the blaNDM-5 gene. We successfully isolated 103 blaNDM-5-positive E. coli strains from 431 intestinal fecal samples obtained from waterfowl across five provincial-level units in China, with all strains exhibiting multidrug resistance (MDR). Notably, the blaNDM-5 gene was identified on plasmids, which facilitate efficient and stable horizontal gene transfer (HGT). Our adaptability assays indicated that while the blaNDM-5-positive plasmid imposed a fitness cost on the host bacteria, the NDM-5 protein was successfully induced and purified, exhibiting significant enzymatic activity. One strain, designated DY51, exhibited a minimum inhibitory concentration (MIC) for imipenem of 4 mg/L, which escalated to 512 mg/L following exposure to increasing imipenem doses. This altered strain demonstrated stable resistance to imipenem alongside improved adaptability, correlating with elevated relative expression levels of the blaNDM-5 and overexpression of efflux pumps. Collectively, this study highlights the horizontal dissemination of the blaNDM-5 plasmid among E. coli strains, confirms the associated fitness costs, and provides insights into the mechanisms underlying the stable increase in antibiotic resistance to imipenem. These findings offer a theoretical framework for understanding the dissemination dynamics of blaNDM-5 in E. coli, which is essential for developing effective strategies to combat carbapenem antibiotic resistance.

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Genomics analysis of KPC-2 and NDM-5-producing Enterobacteriaceae in migratory birds from Qinghai Lake, China

Cited by 2 publications

References 53 publications

Emergence of carbapenem‐resistant Klebsiella pneumoniae species complex from agrifood systems: detection of ST6326 co‐producing KPC‐2 and NDM‐1

Emergence of carbapenem‐resistant Klebsiella pneumoniae species complex from agrifood systems: detection of ST6326 co‐producing KPC‐2 and NDM‐1

Decoding the enigma: unveiling the transmission characteristics of waterfowl-associated blaNDM-5-positive Escherichia coli in select regions of China

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