Genomics analysis of the steroid estrogen-degrading bacterium Serratia nematodiphila DH-S01

Abstract

At present, the oestrogens detected in the environment have been classified as group 1 carcinogens by the World Health Organization. They have obvious effects on organisms at extremely low environmental concentration (1.0 ng/L) and easily accumulate in the ecosystem, which has adverse effects on the environment and ecological health. The use of microbial degradation to remove steroid estrogens from polluted environments has received increasing attention. In this study, a bacterium capable of degrading 17b-estradiol was isolated from a sewage treatment plant in Jilin, China, and identified as Serratia nematodiphila DH-S01. The results of degradation experiments showed that after culturing the bacteria for 4 days, the degradation rate of oestrone and 17b-estradiol at 15 mg/L reached 93.47% and 93.2%, respectively. Genome-wide sequencing showed that the genome of strain DH-S01 consists of a single circular chromosome, 5,256,558 bp in length, which contains 4,874 predicted coding genes. Based on genome annotation, high abundance genes are related to the metabolism of terpenoids and polyketides. Nine types of sterol-and oestrogen-degrading enzymes were annotated in this strain, and the existence and expression of the enzymes were analyzed by polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR). Comparative genomic analysis showed that there are genes encoding eight enzymes in the common genes of the four Serratia strains, highlighting the potential of the other three Serratia strains to degrade steroid estrogen.

Microbial biomass, composition, and functions are responsible for the differential removal of trace organic chemicals in biofiltration systems

“…For instance, the degradation of ibuprofen can be initiated by enoyl-CoA hydratase with the introduction of hydroxyl groups, this enzyme was found to be up-regulated during the biodegradation process (Kjeldal et al 2012). Enoyl-CoA hydratase was also identified in steroid estrogen degradation by bacterium Serratia nematodiphila (Zhao et al 2020). Furthermore, Carmeron et al (2019) reported enoyl-CoA hydratase contributed to biofilm formation and the antibiotic tolerance, which also supported our findings that the high biomass promoted the TOrCs removal.…”

“…2012). Enoyl-CoA hydratase was also identified in steroid estrogen degradation by bacterium Serratia nematodiphila (Zhao et al . 2020).…”

Microbial Biomass, Composition, and Functions Are Responsible for the Differential Removal of Trace Organic Chemicals in Biofiltration Systems: A Batch Study

“…For instance, the degradation of ibuprofen can be initiated by enoyl-CoA hydratase with the introduction of hydroxyl groups, this enzyme was found to be upregulated during the biodegradation process (Almeida et al, 2013). Enoyl-CoA hydratase was also identified in steroid estrogen degradation by bacterium Serratia nematodiphila (Zhao et al, 2020). Furthermore, Cameron et al (2019) reported enoyl-CoA hydratase contributed to biofilm formation and the antibiotic tolerance, which also supported our findings that the high biomass promoted the TOrCs removal.…”

The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001

“…Most estrogen-degrading bacteria have been identified from activated sludge, farmland soil, or compost in sewage treatment plants. At present, more than one hundred estrogen-degrading microorganisms have been reported [ 10 – 34 ], and the estrogen degradation efficiency of some strains can reach more than 90% within a specific time period (Table S1 ). For example, Actinomycetes have very good substrate adaptation to high concentrations of estrogen and exhibit no delay in cell growth.…”