Genomics and nitrogen metabolic characteristics of a novel heterotrophic nitrifying-aerobic denitrifying bacterium Acinetobacter oleivorans AHP123

Zhou, Xiangqun; Wang, Yuanli; Tan, Xiaojie; Sheng, Yequan; Li, Yanbin; Zhang, Qin; Xu, Jun; Shi, Zhengsheng

doi:10.1016/j.biortech.2023.128822

Cited by 40 publications

(3 citation statements)

References 36 publications

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“…The key genes involved in nitrogen removal have been detected to further confirm the HN-AD capacity of HN1 and establish its nitrogen metabolism pathway. Key genes corresponding to hydroxylamine oxidoreductase (HAO) and ammonia monooxygenase (AMO) were absent from the genome of strain HN1, which is similar to other reported HN-AD strains, such as Stutzerimonas frequens TF18 [44], Acinetobacter oleivorans AHP123 [45], and Halomonas venusta SND-01 [22], resulting in the prevention of the complete nitrification pathway. However, the presence of the nxrAB proved that HN1 could convert nitrite to nitrate.…”

Section: Discussionsupporting

confidence: 74%

Characteristics and Mechanism of Heterotrophic Nitrification/Aerobic Denitrification in the Marine Tritonibacter mobilis HN1

Qi,

Zhang,

et al. 2023

JMSE

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This work aimed to reveal the heterotrophic nitrification and aerobic denitrification activities in a salt-tolerant strain, Tritonibacter mobile HN1, isolated from mariculture sludge by using a pure culture method. Strain HN1 showed the removal rates of ammonia, nitrite, nitrate, and total nitrogen of 98.22%, 100%, 95.71%, and 86.63%, respectively, with the carbon source of sodium citrate or sodium succinate, ratio of carbon to nitrogen of 15, salinity of 3%, temperature of 30 °C, shaking speed of 120 rpm, and pH of 8. The genes of narG, narH, narI, nirS, norB, norC, nosZ, glnA, and GltB were found in the genome sequence of strain HN1, confirming the pathways of nitrogen assimilation, heterotrophic nitrification, and aerobic denitrification. In addition, two nitrifying genes, amo and nap, were missing in the genome of Tritonibacter mobile HN1, indicating that HN1 may have novel genes for this function. This study showed that HN1 had the potential to remove nitrogen contaminants in saline environments and was the first Tritonibacter mobilis strain with heterotrophic nitrification and aerobic denitrification capabilities.

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Section: Discussionsupporting

confidence: 74%

Characteristics and Mechanism of Heterotrophic Nitrification/Aerobic Denitrification in the Marine Tritonibacter mobilis HN1

Qi,

Zhang,

et al. 2023

JMSE

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“…Grease as a carbon source could provide additional electron donors for nitrogen metabolism, thereby increasing the nitrogen -removal efficiency ( He et al, 2015 ). Meanwhile, the assimilation pathway was maintained by nitrate reductase ( nasA ) and nitrite reductase ( nirB/nirD ), which reduced NO 3 − − N to NO 3 − − N and NO 3 − − N to NH 4 + -N. Under the catalysis of gdhA, glnA , and gltB genes, NH 4 + -N was converted into biological nitrogen for cell growth and metabolism ( Zhou X. et al, 2023 ). Thus, the assimilation gene was significantly upregulated, indicating that the assimilation pathway became highly active when KFOG served as the carbon source.…”

Section: Resultsmentioning

confidence: 99%

New insight into the metabolic mechanism of a novel lipid-utilizing and denitrifying bacterium capable of simultaneous removal of nitrogen and grease through transcriptome analysis

Tong,

Li,

Qin

et al. 2023

Front. Microbiol.

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IntroductionIssues related to fat, oil, and grease from kitchen waste (KFOG) in lipid-containing wastewater are intensifying globally. We reported a novel denitrifying bacterium Pseudomonas CYCN-C with lipid-utilizing activity and high nitrogen-removal efficiency. The aim of the present study was aim to explore the metabolic mechanism of the simultaneous lipid-utilizing and denitrifying bacterium CYCN-C at transcriptome level.MethodsWe comparatively investigated the cell-growth and nitrogen-removal performances of newly reported Pseudomonas glycinae CYCN-C under defined cultivation conditions. Transcriptome analysis was further used to investigate all pathway genes involved in nitrogen metabolism, lipid degradation and utilization, and cell growth at mRNA levels.ResultsCYCN-C could directly use fat, oil, and grease from kitchen waste (KFOG) as carbon source with TN removal efficiency of 73.5%, significantly higher than that (60.9%) with sodium acetate. The change levels of genes under defined KFOG and sodium acetate were analyzed by transcriptome sequencing. Results showed that genes cyo, CsrA, PHAs, and FumC involved in carbon metabolism under KFOG were significantly upregulated by 6.9, 0.7, 26.0, and 19.0-folds, respectively. The genes lipA, lipB, glpD, and glpK of lipid metabolic pathway were upregulated by 0.6, 0.4, 21.5, and 1.3-folds, respectively. KFOG also improved the denitrification efficiency by inducing the expression of the genes nar, nirB, nirD, and norR of denitrification pathways.ConclusionIn summary, this work firstly provides valuable insights into the genes expression of lipid-utilizing and denitrifying bacterium, and provides a new approach for sewage treatment with reuse of KFOG wastes.

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“…Q-PCR was also performed on the target and internal reference genes of each sample using SYBR Green I. The data were analyzed using the 2 −∆∆CT method [19].…”

Section: Functional Genes Analysismentioning

confidence: 99%

Characteristics of Nitrogen Removal and Functional Gene Transcription of Heterotrophic Nitrification-Aerobic Denitrification Strain, Acinetobacter sp. JQ1004

Hou,

Huang,

Pan

et al. 2024

Water

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In this study, the heterotrophic nitrification–aerobic denitrification strain JQ1004 was investigated in terms of its nitrogen removal mechanism and kinetic properties, laying the foundation for its application in the field of wastewater treatment. Nitrogen balance analysis revealed that the final metabolic product was N2, and approximately 54.61% of N was converted into cellular structure through assimilation. According to the fitting of the Compertz model, the maximum degradation rates of ammonia and nitrate were 7.93 mg/(L·h) and 4.08 mg/(L·h), respectively. A weakly alkaline environment was conducive to N removal, and the sensitivity of functional genes to acidic environments was amoA > nirS > narG. An appropriate increase in dissolved oxygen significantly enhanced heterotrophic nitrification activity, and notably, the denitrification-related functional gene narG exhibited greater tolerance to dissolved oxygen compared to nirS. The transcription level of amoA was significantly higher than that of narG or nirS, confirming that there might have been direct ammonia oxidation metabolic pathways (NH4+→NH2OH→N2) besides the complete nitrification and denitrification pathway. The annotation of nitrogen assimilation-related functional genes (including gltB, gltD, glnA, nasA, nirB, narK, nrtP, cynT, and gdhA genes) in the whole-genome sequencing analysis further confirmed the high assimilation nitrogen activity of the HN-AD strain.

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Genomics and nitrogen metabolic characteristics of a novel heterotrophic nitrifying-aerobic denitrifying bacterium Acinetobacter oleivorans AHP123

Cited by 40 publications

References 36 publications

Characteristics and Mechanism of Heterotrophic Nitrification/Aerobic Denitrification in the Marine Tritonibacter mobilis HN1

Characteristics and Mechanism of Heterotrophic Nitrification/Aerobic Denitrification in the Marine Tritonibacter mobilis HN1

New insight into the metabolic mechanism of a novel lipid-utilizing and denitrifying bacterium capable of simultaneous removal of nitrogen and grease through transcriptome analysis

Characteristics of Nitrogen Removal and Functional Gene Transcription of Heterotrophic Nitrification-Aerobic Denitrification Strain, Acinetobacter sp. JQ1004

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