Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?

Yurchenko, Vyacheslav; Butenko, Anzhelika; Kostygov, Alexei Y.

doi:10.3390/pathogens10091124

Cited by 11 publications

(11 citation statements)

References 153 publications

(196 reference statements)

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“…The catalase gene copy number was analyzed in eight trypanosomatid species with high-quality genomes available [ 46 ]: Blastocrithidia sp., Crithidia bombi , C. expoeki , C. fasciculata , Leptomonas pyrrhocoris , L. seymouri , Novymonas esmeraldas , and Vickermania ingenoplastis ( Table S1 ). We reasoned that using lower-quality draft genomic data is not justified as it may produce artifacts, because of the substantial number of unassembled contigs.…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Three Trypanosomatid Catalases of Different Origin

et al. 2021

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Most trypanosomatid flagellates do not have catalase. In the evolution of this group, the gene encoding catalase has been independently acquired at least three times from three different bacterial groups. Here, we demonstrate that the catalase of Vickermania was obtained by horizontal gene transfer from Gammaproteobacteria, extending the list of known bacterial sources of this gene. Comparative biochemical analyses revealed that the enzymes of V. ingenoplastis, Leptomonas pyrrhocoris, and Blastocrithidia sp., representing the three independent catalase-bearing trypanosomatid lineages, have similar properties, except for the unique cyanide resistance in the catalase of the latter species.

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Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Three Trypanosomatid Catalases of Different Origin

et al. 2021

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“…Despite kinetoplastids having similarities in their genomic organization and cellular structures, they comprise a large variety of organisms, many of them pathogenic, and transmitted by different vectors, causing different diseases in multiple hosts [ 1 ]. Parasites of the genera Leishmania [ 2 ] and Trypanosoma [ 3 ] cause disease in humans, while pathogens of the genus Phytomonas are capable of infecting plants [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Trypanosomatidae Activity of Essential Oils and Their Main Components from Selected Medicinal Plants

et al. 2023

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Kinetoplastida is a group of flagellated protozoa characterized by the presence of a kinetoplast, a structure which is part of a large mitochondria and contains DNA. Parasites of this group include genera such as Leishmania, that cause disease in humans and animals, and Phytomonas, that are capable of infecting plants. Due to the lack of treatments, the low efficacy, or the high toxicity of the employed therapeutic agents there is a need to seek potential alternative treatments. In the present work, the antiparasitic activity on Leishmania infantum and Phytomonas davidi of 23 essential oils (EOs) from plants of the Lamiaceae and Asteraceae families, extracted by hydrodistillation (HD) at laboratory scale and steam distillation (SD) in a pilot plant, were evaluated. The chemical compositions of the EOs were determined by gas chromatography-mass spectrometry. Additionally, the cytotoxic activity on mammalian cells of the major components from the most active EOs was evaluated, and their anti-Phytomonas and anti-Leishmania effects analyzed. L. infantum was more sensitive to the EOs than P. davidi. The EOs with the best anti-kinetoplastid activity were S. montana, T. vulgaris, M. suaveolens, and L. luisieri. Steam distillation increased the linalyl acetate, β-caryophyllene, and trans-α-necrodyl acetate contents of the EOs, and decreased the amount of borneol and 1,8 cineol. The major active components of the EOs were tested, with thymol being the strongest anti-Phytomonas compound followed by carvacrol. Our study identified potential treatments against kinetoplastids.

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“…The number of Leishmania species with sequenced and annotated genomes that are amenable to genetic manipulations and, thus, can be considered as models, remains limited [28]. Moreover, to the best of our knowledge, this list does not include any LRV-containing isolate, precluding detailed molecular studies into the undoubtedly important role viruses play in Leishmania biology and leishmaniasis etiology.…”

Section: Introductionmentioning

confidence: 99%

Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content

et al. 2022

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Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the endosymbiotic Leishmania RNA virus 1 (LRV1) cause more severe form of the disease in a mouse model than those that do not. The presence of the virus was implicated into the parasite’s replication and spreading. In this respect, studying the molecular mechanisms of cellular control of viral infection is of great medical importance. Here, we report ~30.5 Mb high-quality genome assembly of the LRV1-positive L. guyanensis M4147. This strain was turned into a model by establishing the CRISPR-Cas9 system and ablating the gene encoding phosphatidate phosphatase 2-like (PAP2L) protein. The orthologue of this gene is conspicuously absent from the genome of an unusual member of the family Trypanosomatidae, Vickermania ingenoplastis, a species with mostly bi-flagellated cells. Our analysis of the PAP2L-null L. guyanensis showed an increase in the number of cells strikingly resembling the bi-flagellated V. ingenoplastis, likely as a result of the disruption of the cell cycle, significant accumulation of phosphatidic acid, and increased virulence compared to the wild type cells.

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Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?

Cited by 11 publications

References 153 publications

Comparative Analysis of Three Trypanosomatid Catalases of Different Origin

Comparative Analysis of Three Trypanosomatid Catalases of Different Origin

Anti-Trypanosomatidae Activity of Essential Oils and Their Main Components from Selected Medicinal Plants

Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content

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