Genomotyping of Coxiella burnetii Using Microarrays Reveals a Conserved Genomotype for Hard Tick Isolates

Leroy, Quentin; Armougom, Fabrice; Barbry, Pascal; Raoult, Didier

doi:10.1371/journal.pone.0025781

Cited by 16 publications

(10 citation statements)

References 32 publications

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“…Another study, using whole-genome microarrays, compared the genome content of 52 C. burnetii isolates retrieved from hard ticks, mammals and humans, demonstrated the existence of a single conserved genomotype for hard tick isolates. [44].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Infectious Potential of Coxiella burnetii: A Study on Belgian Livestock Isolates

et al. 2013

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Q-fever is a zoonosis caused by the gram-negative obligate intracellular pathogen Coxiella burnetii. Since its discovery, and particularly following the recent outbreaks in the Netherlands, C. burnetii appeared as a clear public health concern. In the present study, the infectious potential displayed by goat and cattle isolates of C. burnetii was compared to a reference strain (Nine Mile) using both in vitro (human HeLa and bovine macrophage cells) and in vivo (BALB/c mice) models. The isolates had distant genomic profiles with one - the goat isolate - being identical to the predominant strain circulating in the Netherlands during the 2007–2010 outbreaks. Infective doses were established with ethidium monoazide-PCR for the first time here applied to C. burnetii. This method allowed for the preparation of reproducible and characterized inocula thanks to its capacity to discriminate between live and dead cells. Globally, the proliferative capacity of the Nine Mile strain in cell lines and mice was higher compared to the newly isolated field strains. In vitro, the bovine C. burnetii isolate multiplied faster in a bovine macrophage cell line, an observation tentatively explained by the preferential specificity of this strain for allogeneic host cells. In the BALB/c mouse model, however, the goat and bovine isolates multiplied at about the same rate indicating no peculiar hypervirulent behavior in this animal model.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Infectious Potential of Coxiella burnetii: A Study on Belgian Livestock Isolates

et al. 2013

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“…Comparative genomic hybridization (CGH), thanks to whole-genome microarray techniques, was performed when genome sequences became available. Different "genomotypes" were defined based on polymorphisms in plasmid open reading frames (ORFs) and chromosomal sequences (166), but this method is time-consuming. Loftis et al have tried to describe the diversity of strains by detection of different plasmid sequences, but this has a poor discriminant power (167).…”

Section: Comparative Genomics and Pangenomic Analysismentioning

confidence: 99%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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Coxiella burnetii is the agent of Q fever, or "query fever," a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection with C. burnetii was the breaking of the old dichotomy between "acute" and "chronic" Q fever. The clinical presentation of C. burnetii infection depends on both the virulence of the infecting C. burnetii strain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients with C. burnetii infection.

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“…Although microarrays are built on the basis of gene family clusters, PanArray uses an approach based and centreed on probes independently of annotation, gene clustering and multi-alignments. This tool works as well for the known isolates as the unknown; it has been tested on 20 isolates of Listeria monocytogenes and also on C. burnetii [28,29] . Finally, obtaining data from the microarray approach requires particular and specific analyses, new genes cannot be found.…”

Section: Toolsmentioning

confidence: 99%

The bacterial pangenome as a new tool for analysing pathogenic bacteria

Rouli

Merhej

Fournier

et al. 2015

New Microbes and New Infections

231

187

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The bacterial pangenome was introduced in 2005 and, in recent years, has been the subject of many studies. Thanks to progress in next-generation sequencing methods, the pangenome can be divided into two parts, the core (common to the studied strains) and the accessory genome, offering a large panel of uses. In this review, we have presented the analysis methods, the pangenome composition and its application as a study of lifestyle. We have also shown that the pangenome may be used as a new tool for redefining the pathogenic species. We applied this to the Escherichia coli and Shigella species, which have been a subject of controversy regarding their taxonomic and pathogenic position.

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Genomotyping of Coxiella burnetii Using Microarrays Reveals a Conserved Genomotype for Hard Tick Isolates

Cited by 16 publications

References 32 publications

In Vitro and In Vivo Infectious Potential of Coxiella burnetii: A Study on Belgian Livestock Isolates

In Vitro and In Vivo Infectious Potential of Coxiella burnetii: A Study on Belgian Livestock Isolates

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

The bacterial pangenome as a new tool for analysing pathogenic bacteria

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