Genotoxic activity of the commercial herbicide containing bifenox in bovine peripheral lymphocytes

Šiviková, Katarı́na; Dianovský, Ján

doi:10.1016/s1383-5718(98)00184-3

Cited by 16 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[28] According to Preston et al, [29] in case of cytotoxicity induced by non-clastogenic compounds, it can be actually a confirmation that the metabolic activation of the test compound had occurred. Similar to our experiment, a slight (non-significant) increase of CAs in 24h assay and induction of cytotoxicity were observed byŠiviková et al [30] in bovine cultured lymphocytes after treatment with the commercial triazole herbicide containing bifenox. In the 48 h test, the authors detected a clastogenic effect at only one of all tested concentrations.…”

Section: Polyploidiessupporting

confidence: 92%

Effect of triazole pesticide formulation on bovine culture cells

Holečková

Šiviková

Dianovský

et al. 2013

Journal of Environmental Science and Health, Part B

Self Cite

View full text Add to dashboard Cite

To date, most data about the possible genotoxic effect of triazole pesticides are focused on laboratory animals resulting in limited information on further non-target organisms such as cattle. The objective of the present study was to investigate the effect of triazole (tebuconazole/prothioconazole) fungicide formulation on the induction of chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and DNA fragmentation in bovine cultured lymphocytes. Our results showed that the fungicide formulation did not induce significant number of CAs in bovine cells after 24 h treatment. Nevertheless, the dose-dependent reduction of mitotic division was observed, with the strongest effect at 30.0 μg mL(-1) in both donors (P < 0.01 and P < 0.001, respectively). Prolonged 48 h exposure caused the increased level of breaks in treated cultures (3.0-15.0 μg mL(-1); P < 0.05) and significant decrease in mitotic index (MI). The tested fungicide failed to produce any statistical changes in the SCE frequency neither after 24 h nor 48 h treatment. However, the significant decline of the proliferation index (PI) was observed after 24 h indicating the fungicide influence on cell cycle kinetics. Prolonged 48 h exposure caused cytotoxicity reflecting in lower PI value relative to control mainly at the highest fungicide concentrations (30.0 μg mL(-1), P < 0.001). Using painting probes for bovine chromosomes 1, 5 and 7 (BTA1, BTA5 and BTA7) only low levels of aneuploidies were detected. Significant increase of polyploidy cells (P < 0.05) was induced by a 3.0 μg mL(-1) dose of the fungicide after 48 h. DNA fragmentation assay didn't reveal the presence of DNA nucleosome ladder in cell cultures at any time (24 h and 48 h) and fungicide concentration.

show abstract

Section: Polyploidiessupporting

confidence: 92%

Effect of triazole pesticide formulation on bovine culture cells

Holečková

Šiviková

Dianovský

et al. 2013

Journal of Environmental Science and Health, Part B

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previously we reported the results on chromosome aberrations and sister chromatid exchanges in bovine blood lymphocytes exposed to the herbicide in different sampling times -for the last 2h, 24h and 48h of cultivation, respectively (·iviková and Dianovsk˘ 1999). The statistically significant genotoxic effect was obtained in the sister chromatid assay after exposure to the herbicide for 24 h only.…”

mentioning

confidence: 92%

Mitotic Index and Cell Proliferation Kinetics as Additional Variables for Assessment of Genotoxic Effect of The Herbicide Modown

Šiviková¹,

Dianovský²

2000

Acta Vet. Brno

View full text Add to dashboard Cite

The in vitro effect of the herbicide Modown (with active component bifenox) was tested for the ability to influence cell proliferation of PHA-stimulated bovine peripheral lymphocytes. Mitotic (MI) and proliferation (PI) indices were determined as an alternative for the screening of the cytostatic activity. The herbicide Modown exerted a clear effect on the inhibition of MI and PI over a concentration-tested range of 25 µg/ml to 1000 µg/ml. An expressive proliferation delays was found after the treatment with herbicide at a dose of 250 µg/ml (P < 0.001), while the higher doses of 500 and 1000 µg/ml had caused nearly complete mitotic inhibition in each donor (P < 0.05 and P < 0.001, respectively). A correlation between the PI and MI inhibition refers rather to cytostatic than cytotoxic effects of the herbicide. The results support the possibility of immunosuppression by herbicide exposure. Herbicide Modown, bovine peripheral lymphocytes, cell proliferation, cytostatic effect

show abstract

“…1) is non-persistent but genotoxic (Sivikova and Dianovsky 1999) herbicide and thus there is an ever increasing demand for sensitive and selective analytical methods enabling its trace determination in both drinking and surface waters.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of the herbicide Bifenox in drinking and river water using a silver solid amalgam electrode

et al. 2009

View full text Add to dashboard Cite

A new method for the determination of submicromolar and nanomolar concentrations of herbicide Bifenox using differential pulse voltammetry at a mercury meniscus modified silver solid amalgam electrode is described. This new type of working electrode is non-toxic, environmentally friendly, and compatible with principles of green analytical chemistry, and its surface can be easily electrochemically renewed in the case of passivation. The newly developed direct differential pulse voltammetric determination of Bifenox gives limit of determination 6.7Á10 -7 and 6.9Á10 -7 M in drinking and river water, respectively. Both the selectivity and the sensitivity can be further increased by preliminary separation and preconcentration using solid phase extraction. This combination enables to reach limit of determination 0.9 9 10 -9 and 1.5 9 10 -9 M for drinking and river water, respectively. Because of lower running and investment costs, this electroanalytical method can be used for large scale monitoring of possible water contamination with tested herbicide.

show abstract

Genotoxic activity of the commercial herbicide containing bifenox in bovine peripheral lymphocytes

Cited by 16 publications

References 20 publications

Effect of triazole pesticide formulation on bovine culture cells

Effect of triazole pesticide formulation on bovine culture cells

Mitotic Index and Cell Proliferation Kinetics as Additional Variables for Assessment of Genotoxic Effect of The Herbicide Modown

Voltammetric determination of the herbicide Bifenox in drinking and river water using a silver solid amalgam electrode

Contact Info

Product

Resources

About