Genotoxic and cytotoxic effect of chitinase against Corcyra cephalonica larvae under laboratory conditions

Vijayakumar, N.; Nalini, Madanagopal; Rajkuberan, Chandrasekaran; Faruck, Lukmanul Hakkim; Bakshi, Hamid A.; Sangilimuthu, Alagar Yadav

doi:10.1007/s42690-021-00478-8

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…The addition of R. iracundus venom to S2 insect cells caused 99% cell lysis within 30 s, demonstrating the potency of R. iracundus venom to lyse and liquify their prey. In our transcriptome and proteome data we found digestive enzymes such as S1 proteases, S1 protease + CUB, and also chitinases which are, possibly among other molecular components, responsible for the observed insect softening and cell lysis [26,62].…”

Section: Primary Functions Of Reduviid Venom: Capturing and Feeding On Arthropod Preymentioning

confidence: 83%

Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

et al. 2021

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Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized and systemic reactions in vertebrates. However, assassin bug venoms remain poorly characterized. We collected the venom from the assassin bug Rhynocoris iracundus and investigated its composition and bioactivity in vitro and in vivo. It caused lysis of murine neuroblastoma, hepatoma cells, and healthy murine myoblasts. We demonstrated, for the first time, that assassin bug venom induces neurolysis and suggest that it counteracts paralysis locally via the destruction of neural networks, contributing to tissue digestion. Furthermore, the venom caused paralysis and melanization of Galleria mellonella larvae and pupae, whilst also possessing specific antibacterial activity against Escherichia coli, but not Listeria grayi and Pseudomonas aeruginosa. A combinatorial proteo-transcriptomic approach was performed to identify potential toxins responsible for the observed effects. We identified neurotoxic Ptu1, an inhibitory cystin knot (ICK) toxin homologous to ω-conotoxins from cone snails, cytolytic redulysins homologous to trialysins from hematophagous kissing bugs, and pore-forming hemolysins. Additionally, chitinases and kininogens were found and may be responsible for insecticidal and cytolytic activities. We demonstrate the multifunctionality and complexity of assassin bug venom, which renders its molecular components interesting for potential biomedical applications.

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Section: Primary Functions Of Reduviid Venom: Capturing and Feeding On Arthropod Preymentioning

confidence: 83%

Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

et al. 2021

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Hemocytic cell line from the moth Glyphodes pyloalis (Lepidoptera: Crambidae) response to essential oils from Artemisia annua (Asterales: Asteraceae)

Oftadeh

Sendi

Valizadeh

et al. 2022

In Vitro Cell.Dev.Biol.-Animal

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Genotoxic effect of two environmentally safe doses of cadmium on the hepato-nephrocytic system of Bombus atratus forager workers

Boeing,

Provase,

Tsukada

et al. 2024

Environmental Toxicology and Pharmacology

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Genotoxic and cytotoxic effect of chitinase against Corcyra cephalonica larvae under laboratory conditions

Cited by 4 publications

References 33 publications

Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Hemocytic cell line from the moth Glyphodes pyloalis (Lepidoptera: Crambidae) response to essential oils from Artemisia annua (Asterales: Asteraceae)

Genotoxic effect of two environmentally safe doses of cadmium on the hepato-nephrocytic system of Bombus atratus forager workers

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