Genotoxic and mutagenic effects of Atrazine Atanor 50 SC on Dendropsophus minutus Peters, 1872 (Anura: Hylidae) developmental larval stages

Gonçalves, Macks Wendhell; Campos, Calebe Bertolino Marins de; Guerra, Vinícius; Cruz, Aparecido Divino da; Marco, Paulo De; Silva, Daniela de Melo e

doi:10.1016/j.chemosphere.2017.05.078

Cited by 26 publications

(13 citation statements)

References 42 publications

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“…In this study, the commercial form of marvel herbicide showed to be highly genotoxic, just as previously reported on evaluations of its individual compounds, atrazine and dicamba, in different species (Campos-Pereira et al, 2012;Gonçalves et al, 2017;Huang et al, 2015;Larramendy et al, 2010;Perocco et al, 1990;Sharma & Vig 2012;Song et al, 2009;Srivastava & Mishra, 2009;Ruiz et al, 2014).…”

Section: Discussionsupporting

confidence: 78%

“…The Environmental Protection Agency (US-EPA, 2009) decided to reassess the risks of atrazine to the health and the environment; additionally, Srivastava & Mishra (2009) reported its effect in altering the mitotic index, increasing the number of chromosomal aberrations in plants. Genotoxic activity of atrazine was clearly demonstrated in different bioassays using earthworm, quail, micro-algae, fishes, anurans, rodents, human lymphocytes, HepG2 cells, and plants (Adeyemi et al, 2015;Campos-Pereira et al, 2012;García-Villada & Reboud, 2007;Gonçalves et al, 2017;Nwani et al, 2011;Powell et al, 2011;Song et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Concentration-associated genotoxicity of the herbicide marvel in Oreochromis niloticus erythrocytes and in human and mouse Balb/c lymphocytesalb/c

Silva

Arámbula²,

Ramírez³

et al. 2022

Acta Universitaria

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The genotoxic activity of various concentrations of the marvel herbicide (0.76 μg/1 ml, 7.68 μg/1 ml, 76.8 μg/1 ml, 768 μg/1 ml) was evaluated in lymphocytes of humans and in Balb/c strain mice, as well as in erythrocytes of tilapia (Oreochromis niloticus). They were exposed to each of the concentrations for 6 h, and then genetic damage was evaluated using the comet assay. All concentrations tested induced significant genetic damage compared to the negative control (p < 0.01). On the other hand, there were no differences between the blood cells of the organisms studied. Tail length and tail moment increased starting from 0.76 μg/1 ml concentration, which is the lowest concentration reported to cause any genetic damage. Data suggest that the reported differences regarding the genotoxicity of marvel and its active ingredients are due to the marked differences between the concentrations studied and the adjuvants present in the commercial formulations.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Concentration-associated genotoxicity of the herbicide marvel in Oreochromis niloticus erythrocytes and in human and mouse Balb/c lymphocytesalb/c

Silva

Arámbula²,

Ramírez³

et al. 2022

Acta Universitaria

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“…The conducted studies mainly focused on the evaluation of environmental stressors such as agrochemicals and heavy metals in vivo. [223,[227][228][229][230][231][232][233][234][235][236][237], fungicides (captan) [238], pesticides [239,240], antiparasitic agents [239], persistent organic pollutants (POPs) [219] as well as other agrochemicals [241] and poultry litter [242]. These studies indicated the DNA damaging effects of selected chemicals as well as using amphibians as sentinel organisms for the evaluation of environmental pollution.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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“…In contrast, the laboratory analyses have used concentrations of up to 35 mg/L [9]. Atrazine has been shown to affect the morphology of the oxygen-carrying cells in the circulatory system, causing alterations to the membrane, nucleus, and cytoplasm in erythrocytes from Lithobates catesbeianus [10], Dendrophryniscus minutus [11], Rhinella schneideri [12], Anaxyrus americanus, Xenopus laevis [13], and Lithobates pipiens [14]. Several of these authors have also determined that an increase in micronucleus frequency is associated with liver damage [15,16].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of the Atrazine Herbicide on Erythrocytes and Liver Damage in Lithobates spectabilis

Méndez-Tepepa,

Hernández-Pérez,

Juárez-Santacruz

et al. 2023

Fishes

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In Mexico, atrazine is one of the herbicides most widely authorized and used in different irrigation districts. Atrazine is a herbicide that contaminates aquatic systems. Previous studies have shown that atrazine causes damage to red blood cells and liver tissue in different aquatic species, including abnormalities in melanomacrophages. However, more information on amphibians is needed, since most of these studies have been done on fish. Furthermore, no study has determined the effect of atrazine on species native to Mexico. Therefore, in this study, we asked what the effects of atrazine are on the erythrocytes and melanomacrophages of the liver in the male frog (Lithobates spectabilis). In the present study, we analyzed (1) the cytotoxicity of atrazine using the micronucleus test, (2) the area of melanomacrophage centers and the presence of melanin, and (3) the characterization of liver damage using histological techniques. Our results show that atrazine is cytotoxic to erythrocytes, increases the area of and melanin presence in melanomacrophage centers, and causes liver damage in male L. spectabilis. Therefore, hepatotoxicity and cytotoxicity are indicators of environmental stress. We suggest monitoring Mexico’s aquatic systems and further analyzing atrazine effects and other pollutants on native species.

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Genotoxic and mutagenic effects of Atrazine Atanor 50 SC on Dendropsophus minutus Peters, 1872 (Anura: Hylidae) developmental larval stages

Cited by 26 publications

References 42 publications

Concentration-associated genotoxicity of the herbicide marvel in Oreochromis niloticus erythrocytes and in human and mouse Balb/c lymphocytesalb/c

Concentration-associated genotoxicity of the herbicide marvel in Oreochromis niloticus erythrocytes and in human and mouse Balb/c lymphocytesalb/c

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Cytotoxic Effects of the Atrazine Herbicide on Erythrocytes and Liver Damage in Lithobates spectabilis

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