Genotoxic biomonitoring of agricultural workers exposed to pesticides in the north of Sinaloa State, Mexico

Martínez-Valenzuela, Carmen; Gómez‐Arroyo, Sandra; Villalobos‐Pietrini, Rafael; Waliszewski, Stefan M.; Calderón-Segura, María Elena; Félix-Gastélum, Rubén; Álvarez-Torres, Armando

doi:10.1016/j.envint.2009.07.010

Cited by 75 publications

(50 citation statements)

References 37 publications

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“…For instance, Ç elik et al [32] and Burgaz et al [36] found a significant increase in the frequency of MNC in smokers compared with non-smokers, whereas Bolognesi et al [37] and Martino-Roth et al [38] reported a small decrease in the frequency of MNC in smokers, although this was not significant. Similar to our observations, several authors also found that the occurrence of MNC was not significantly associated with smoking status in individuals occupationally exposed to fuel derivatives [21,22,31,33] or other potentially carcinogenic substances, such as pesticides [23,24]. Regarding ONA, and fully in line with our observations, Martins et al [22] found that tobacco smoking did not significantly interfere with the cytotoxicity induced by petroleum derivates in buccal mucosa cells or in the lateral border epithelium of the tongue in petrol-station attendants.…”

Section: Discussionsupporting

confidence: 91%

“…Alcohol is also described as a genotoxic substance [39], but in this study, as well as in those of others [21,23,31,32,40] its consumption was not significantly associated with the increase in frequency of MNC or ONA in the buccal epithelium.…”

Section: Discussioncontrasting

confidence: 56%

“…The micronucleus assay with exfoliated cells is emerging as a preferential method to measure this type of damage [11]; it is considered a sensitive and non-invasive method for monitoring DNA damage in human populations [15,20]. Recently, such approaches have been frequently used in bio-monitoring studies of occupational exposure to carcinogenic substances [e.g., [21][22][23][24][25], but to our knowledge no study has been done for thermoelectric power-plant workers exposed to VOCs derived from fuel oil. Thus, the main objective of this study is to assess the association between such occupational exposure to VOCs and the frequency of genotoxic and cytotoxic events in the human buccal epithelium.…”

Section: Introductionmentioning

confidence: 99%

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Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Garcia

Linhares

Amaral

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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a b s t r a c tThermoelectric power-plant workers are constantly exposed to high levels of potentially genotoxic gaseous substances, such as volatile organic compounds (VOCs) from the combustion of fuel oil or the processing of naphtha. The aim of the present study was to estimate the association between such occupational exposure and the frequency of micronucleated cells and cells with other nuclear anomalies.Buccal epithelial cells were collected from a total of 44 power-plant workers (exposed group) and 47 administrative workers (non-exposed group), and examined for the frequency of micronucleated cells (MNC) and of cells with other nuclear anomalies (ONA: pyknosis, karyolysis, and karyorrhexis) by means of the micronucleus assay. The frequencies of MNC and ONA per 1000 cells in the exposed group (1.8‰ and 82.4‰, respectively) were significantly higher than in the non-exposed group (0.2‰ and 58.3‰, respectively). The exposed group had a twelve-fold increase in risk for formation of MNC compared with non-exposed individuals (RR = 12.1; 95% CI, 5.0-29.2; P < 0.001). The confounding factors analyzed (age, smoking status, alcohol consumption, and mouthwash use) did not show any significant association with the frequency of MNC or ONA.The findings of this study show that workers from power plants exposed to VOCs have a significantly elevated risk for DNA damage. Therefore, bio-monitoring of DNA damage is recommended for this group of workers.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Garcia

Linhares

Amaral

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Some biomonitoring studies found positive association of age (Ozkul et al,1997;Moretti et al, 2000), sex (Betti et al, 1994;Bajpayee et al, 2002;Fenech et al, 2003), smoking (Burgaz et al, 1999;Hoffmann et al, 2005), alcohol intake (Ramirez et al, 2002) and tobacco chewing (Nair et al, 1991;Trivedi et al, 1993) while some studies observed no association of age (Zhu et al, 1999;Konapaka et al, 2003), sex (Anderson et al, 1993;Zhu et al, 1999;Konapaka et al, 2003), smoking (Moller et al, 2006), alcohol intake (Celik et al, 2007;Martinez-Valenzuela et al, 2009) with genetic damage. In our study, we did not observe any significant association of age, sex and consumption habits (smoking and alcohol use) with genetic damage.…”

Section: Discussionmentioning

confidence: 99%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coaltar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

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“…A study has indicated that frequent and indiscriminate use of pesticides has produced genotoxic damage subsequent to occupational exposure to these substances 25 . However, few such evaluations have been made among fetuses and newborns: such studies have almost always focuses on adults exposed directly or indirectly.…”

Section: It Has Been Demonstrated That Medullary Hematopoiesismentioning

confidence: 99%

Hepatic damage in newborns from female rats exposed to the pesticide derivative ethylenethiourea

et al. 2012

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PURPOSE:To evaluate hepatic morphological-histological abnormalities in newborns from female rats exposed to ethylenethiourea. METHODS:A randomized study was conducted on fifty-five newborn Wistar rats were studied: 34 in the experimental group, whose mothers had been exposed to 1% ethylenethiourea; and 21 in the control group, whose mothers had received 0.9% physiological solution. The solution was administered via gavage on the 11 th day of gestation. Cesarean section was performed on the 20 th day of gestation. The newborns' livers were examined and any morphological-histological abnormalities were registered. The presence of megakaryocytes was quantified in 50 microscope fields, as the total number of these cells per mm 2 . RESULTS:The entire experimental group presented abnormalities of embryonic formation, with musculoskeletal anomalies, digestive system anomalies, hepatic congestion and friability, hydrops and delayed intrauterine growth. The histopathological analysis showed that morphological-histological hepatic destructuring had occurred in all entire experimental with removal of the hepatic trabeculae and severe hepatic megakaryocytosis. The mean megakaryocyte density ranged from 107.9 to 114.2 per mm 2 , and it was eight times greater than in the control group, thus characterizing a situation of extramedullary hematopoiesis. CONCLUSION:The fetal exposure to ethylenethiourea caused hepatic damage characterized by severe extramedullary hematopoiesis.Key words: Pesticides. Ethylenethiourea. Liver. Hematopoiesis, Extramedullary. Pregnancy, Animal. Animals, Newborn. Rats. RESUMO OBJETIVO:Avaliar alterações hepáticas morfohistológicas em recém-nascidos de ratas prenhes expostas à etilenotioureia. MÉTODOS:Realizado ensaio randomizado em animais de experimentação, onde foram estudados 55 recém-nascidos de ratas Wistar, 34 do Grupo Experimento, expostas a etilenotioureia 1% e 21 do Grupo Controle, em que a rata prenhe recebeu solução fisiológica 0,9%, ambos por gavagem no 11º dia de gestação. Realizada no 20º dia de gestação cesariana, analisados os fígados dos recém-nascidos e registradas as alterações morfohistológicas. Realizou-se a quantificação dos megacariócitos em 50 campos microscópicos, avaliando a quantidade total destas células por mm². RESULTADOS:Todos os recém-nascidos do Grupo Experimento apresentaram alterações na formação embrionária, com anomalias musculoesqueléticas, anormalidades do sistema digestório, congestão e friabilidade hepática, hidropisia e crescimento intrauterino retardado. A análise histopatológica mostrou desestruturação hepática morfohistológica em todos os recém-nascidos expostos à etilenotioureia, com destrabeculação dos hepatócitos e intensa megacariocitose hepática, apresentando média da densidade deLemos PVRB et al. -Acta Cirúrgica Brasileira -Vol. 27 (12) 2012megacariócitos de 107,9 até 114,2 por mm 2 sendo cerca de oito vezes maior que no Grupo Controle, caracterizando hematopoese extramedular. CONCLUSÃO:A exposição fetal a etilenotioureia provocou danos hepát...

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Genotoxic biomonitoring of agricultural workers exposed to pesticides in the north of Sinaloa State, Mexico

Cited by 75 publications

References 37 publications

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Hepatic damage in newborns from female rats exposed to the pesticide derivative ethylenethiourea

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