Genotoxic effects in the Eastern mudminnow (Umbra pygmaea L.) after exposure to Rhine water, as assessed by use of the SCE and Comet assays: A comparison between 1978 and 2005

Alink, G.M.; Quik, J.T.K.; Penders, E.; Spenkelink, A.; Rotteveel, Serge; Maas, J.L.; Hoogenboezem, Wim

doi:10.1016/j.mrgentox.2007.03.011

Cited by 37 publications

(14 citation statements)

References 26 publications

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“…All the heavy metals studied were found in higher amounts than the WHO permissible limits. The studies of Alink et al (2007) and Obiakor et al (2010) strongly corroborate the findings of the present study as the River Chenab is heavily polluted by the industry in the city of Faisalabad. A huge population depends on the water from the River Chenab as this body of water largely contributes to agriculture and fisheries.…”

Section: Discussionsupporting

confidence: 91%

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Habitat induced mutational effects and fatty acid profile changes in bottom dweller <em>Cirrhinus mrigala</em> inhabitant of river Chenab

Hussain¹,

Sultana²,

Sultana³

et al. 2015

Grasas y Aceites

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SUMMARY:Fish from the river Chenab were analyzed for DNA damage by the Comet and Micronucleus assays. The fatty acid profile was determined by gas chromatography using a Flame Ionization Detector. Atomic absorption spectrophotometry showed significant (p<0.05) levels of contamination due to Cd, Hg, Cu, Mn, Zn, Pb, Cr, Sn and phenols in the fish habitat. The Comet assay revealed significant (p<0.05) DNA damage in Cirrhinus mrigala of 37.29±2.51%, 34.96±2.53% and 38.80±2.42% in the comet tail, in the tail moment of 23. 48±3.90, 19.78±4.26 and 14.30±1.82, in the olive moment of 16.22±2.04, 13.83±1.96 10.99±0.90, respectively, from three experimental sites. The micronucleus assay showed a high frequency of single micronucleus induction of 44.80±3.73, double induction of 06.20±0.97 and nuclear abnormalities of 09.60±1.72, as calculated in a thousand cells. C8:0, C12:0, C20:0, C16:1(n-7), C16:1(n-9), C20:1(n-9), C18:2(n-6), C18:4(n-3), C20:5(n-3), C22:4(n-6) fatty acids were found missing in the fish with a higher intensity of DNA damage but were found in optimal amounts both in farmed and wild fish from non polluted habitats. A highly significant (p<0.01) correlation was also found in saturated, unsaturated fatty acids and DNA damage and habitat. The present study revealed that the habitat of even lower intensity pollutions not only induce DNA damage but also confiscate essential fatty acids of the omega series and reduce the amount of unsaturated fatty acids for which fish is preferred over other animals.KEYWORDS: Cirrhinus; DNA damage; Fatty acids; Habitat; Niche; Pollution RESUMEN: Efectos inducidos por el hábitat sobre mutaciones y perfil de ácidos grasos en carpas mrigala que habitan en el fondo del río Chenab. Los daños en el ADN de los peces del río Chenab se analizaron mediante la técnica del "ensayo del cometa" y el ensayo de micronúcleos. El perfil de ácidos grasos se determinó mediante cromatografía de gases con detector de ionización de llama. La espectrofotometría de absorción atómica mostró diferencias significativas (p<0.05) de los niveles de contaminación por Cd, Hg, Cu, Mn, Zn, Pb, Cr, Sn y fenoles en el hábitat de los peces. El ensayo del cometa mostró daños significativos (p<0,05) en el ADN de las carpas mrigala de tres sitios experimentales: 37,29±2,51%, 34,96±2,53 y 38,80±2,42% del ADN en la cola del cometa, momento de la cola con 23,48±3,90, 19,78±4,26 y 14,30±1,82% y cola media con 16,22±2,04, 13,83±1,96 10,99±0,90. Los ensayos de micronúcleos mostraron una alta frecuencia de inducción de micronú-cleos como única 44,80±3,73, 06,20±0,97 y doble anomalías nucleares 09,60±1,72 calculados en miles de células. C8:0, C12:0, C20:0, C16:1(n-7), C16:1 (n-9), C20:1(n-9), C18:2(n-6), C18:4(n-3), C20:5(n-3) y C22:4(n-6) se mostraron como ácidos grasos que faltan en el pescado que tiene una mayor intensidad de daño en el ADN, aunque se encuentran en cantidades óptimas, tanto en los peces de piscifactoría como en los salvajes donde no hay hábitats contaminados. Se encontró una correlación altamente significa...

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Section: Discussionsupporting

confidence: 91%

“…The frequencies of micronuclei in erythrocytes were detected under a Binocular microscope at T1200x magnification. The erythrocytes with intact cellular and nuclear membranes were counted (Alink et al, 2007;Obiakor et al, 2010).…”

Section: Dna Damagementioning

confidence: 99%

Habitat induced mutational effects and fatty acid profile changes in bottom dweller <em>Cirrhinus mrigala</em> inhabitant of river Chenab

Hussain¹,

Sultana²,

Sultana³

et al. 2015

Grasas y Aceites

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“…Cells from different tissues or different species can differ substantially in tail length [35]. Still, tail length was, as a parameter, used in studies of genotoxicity in fish tissues [39][40][41][42]. Nevertheless, other parameters, especially, tail intensity and tail moment, were more extensively used in similar works [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the tissue selection for the analyses, three cell types were selected, following previous research, erythrocytes, liver and gills [31,42,[46][47][48]. However, different tissues can accumulate pollutants to different degrees, depending on their biochemical characteristics [49].…”

Section: Discussionmentioning

confidence: 99%

Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences

et al. 2013

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Genotoxic potential of waters in six rivers and reservoirs from Serbia was monitored in different tissues of chub (Squalius cephalus L. 1758) with the alkaline comet assay. The comet assay, or a single cell gel electrophoresis, has a wide application as a simple and sensitive method for evaluating DNA damage in fish exposed to various xenobiotics in the aquatic environment. Three types of cells, erythrocytes, gills and liver cells were used for assessing of DNA damage. Images of randomly selected cells were analyzed with a fluorescence microscope Leica and image analysis by software (Comet Assay IV Image analysis system, PI, UK). Three parameters (tail length -l, tail intensity -i and Olive tail moment -m) were analyzed on 1750 nuclei per cell type. The procedure for sum of ranking differences (SRD) was implemented to compare different types of cells and different parameters for estimation of DNA damage.Regarding our nine different estimations of genotoxicity: tail length, intensity and moment in erythrocytes (rel, rei, rem), liver cells (rll, rli, rlm) and gill cells (rgl, rgi, rgm) SRD procedure has shown that the Olive tail moment and tail intensity are (almost) equally good parameters; the SRD value was lower for the tail moment and tail intensity than for tail length in case of all types of cells. The least reliable parameter was rel; close to the borderline case were rei, rll, and rgl (~5% probability of random ranking).

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“…Actually, there have been several reports on the melamine contamination of groundwater near chemical plants (Malina, 2004;Alink et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Treatment of Melamine by GAC Adsorption According to Adsorbent Size: Kinetics and Dispersion-Diffusion

Lee¹,

Lee²,

Han³

2013

Journal of Soil and Groundwater Environment

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Adsorption of melamine was examined using columns packed with granular activated carbon (GAC). Raw GAC was sieved with 20, 40, 60 and 80 mesh to determine the influence of adsorbent particle size on reaction and diffusion. The mass ratio of the adsorption capacity of GAC for melamine ranged from 9.19 to 11.06%, and adsorption rates increased with decreasing particle size within this range. Rate constants between 3.295~4.799 min −1 were obtained using a pseudofirst-order equation that was used to determine adsorption kinetics. A surface diffusion model was adapted to take into account the unsteady-state equation of a spherical adsorbent by converting the surface concentration from a constant to a variable governed by a dispersion equation. The calculated values were fit with the experimental results by using the diffusion coefficients as regression parameters. The modified equation exhibited a more precise agreement with respect to the sum of the absolute error (SAE).

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Genotoxic effects in the Eastern mudminnow (Umbra pygmaea L.) after exposure to Rhine water, as assessed by use of the SCE and Comet assays: A comparison between 1978 and 2005

Cited by 37 publications

References 26 publications

Habitat induced mutational effects and fatty acid profile changes in bottom dweller <em>Cirrhinus mrigala</em> inhabitant of river Chenab

Habitat induced mutational effects and fatty acid profile changes in bottom dweller <em>Cirrhinus mrigala</em> inhabitant of river Chenab

Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences

Treatment of Melamine by GAC Adsorption According to Adsorbent Size: Kinetics and Dispersion-Diffusion

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