Genotoxic effects of<i>Heracleum sosnowskyi</i>in the<i>Allium cepa</i>test

Pesnya, D. S.; Romanovsky, Anton V.; Serov, Dmitriy A.; Poddubnaya, Nadezhda

doi:10.1080/00087114.2016.1272313

Cited by 14 publications

(10 citation statements)

References 47 publications

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“…According to Leme and Marin-Morales [18], chromosomal breaks and bridges indicate a clastogenic action of the toxicant on the biotest, whereas lost and late chromosomes, stickiness, or c-metaphases result in aneugenic effects. Thus, coumarin A1 caused aneugenic effects in meristematic cells of L. sativa, evident due to the high frequency of stickiness and lost chromosomes promoted by the 50 µg mL −1 concentration [18,19].…”

Section: Discussionmentioning

confidence: 99%

“…Studies of germination parameters and initial seedling growth can verify the biological effects of substances, including coumarins, by elucidating possible phytotoxic effects on the morphophysiology of plant species [5,[15][16][17]. Correlating these phytotoxicity studies with changes in the cell cycle and behavior of the chromosomal complement results in a deeper understanding of the mechanisms of action of coumarins, making it possible to verify cytogenotoxicity as a biological effect of these substances [18,19]. In addition, studies of the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) can infer the occurrence of possible oxidative stress on plants exposed to coumarins and their derivatives.…”

Section: Introductionmentioning

confidence: 99%

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Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Govêa

Pereira

Assis

et al. 2020

Plants

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Coumarins are widely distributed substances in plant species that promote phytotoxic effects, allowing them to be exploited as herbicides less harmful to the environment, since many invasive species have demonstrated resistance to commercially available products. The derived coumarins used in this study had not been tested in plant models and their effect on plants was unknown. The objective of this study was to evaluate the phytotoxic action of these coumarins in bioassays with Lactuca sativa L., in order to select the most responsive substance whose toxicity was best elucidated by chromosomal complement and enzymatic antioxidant metabolism studies. From the phytotoxicity assays, coumarin 8-methoxy-2-oxo-6-(prop-2-en-1-yl)-2H-chromene-3-carboxylic acid (A1), reported here for the first time, was selected as the most responsive and caused a reduction in the following parameters: number of normal seedlings, fresh biomass, root length and shoot length. Subsequent studies demonstrated that this coumarin is cytogenotoxic due to damage caused to the cell cycle and the occurrence of chromosomal abnormalities. However, it did not interfere with antioxidant enzyme activity and did not cause lipid peroxidation. The changes caused by coumarin A1 described herein can contribute to better understanding the allelochemical actions of coumarins and the potential use of these substances in the production of natural herbicides.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Govêa

Pereira

Assis

et al. 2020

Plants

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“…A simple search on Google scholar on 21 June 2018 with the key "Allium test" produced 3170 hits, showing the wide use of the test in a variety of different investigations. A few examples are studies examining the effects of toxic plants (Khan et al 2010;Pesnya et al 2017), nanoparticles (Yekeen et al 2017), synthetic plant hormones such as etephon (Yavuz et al, 2017), industrial waste (Firbas and Amon 2017;Dutta et al 2018), aquatic environmental samples (Firbas 2015), fungal extracts (Dávila Giraldo et al 2018), herbicides (de Souza et al 2016), insecticides (Souza et al 2017), fungicides (Dane and Dalgiç 2005), pesticides (Fatma et al 2018), radioactivity (Evseeva et al, 2003) and heavy metals (Liu and Kottke 2003), and the mitigating effects of antioxidant or vitamins in case of treatment with genotoxic agents (Asita Okorie et al 2017).…”

Section: Introductionmentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student's t, and the nonparametric tests Kruskal-Wallis and Mann-Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.

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“…Medicinal plants, although used for their therapeutic properties, also exhibit dose dependent toxic effects. Allium cepa root tip meristematic cells assay easily determines dose dependent cytotoxicity and genotoxicity of medicinal plant extracts and plant-derived compounds [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

An evidence based efficacy and safety assessment of the ethnobiologicals against poisonous and non-poisonous bites used by the tribals of three westernmost districts of West Bengal, India: Anti-phospholipase A2 and genotoxic effects

et al. 2020

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Introduction To explore the ethnobiological wisdom of the tribals of three western districts of West Bengal, India against poisonous and non-poisonous bites and stings, a quantitative approach was adopted. These age-old yet unexplored knowledge can be utilized in finding lead-molecules against poisonous and non-poisonous animal-bites. Further, an evidence-based approach is needed to assess the venom-neutralization ability of plants by experimental studies. Materials and methods During 2008–2009 and 2012–2017, 11 ethnomedicinal surveys were carried out to explore the use of medicinal flora and fauna via conducting open semi-structured interviews with 47 traditional healers (THs) or informants. The retrieved dataset was statistically evaluated using seven quantitative-indexes: use-value (UV), informants'-consensus-factor (ICF), fidelity-level (FL), relative-importance (RI), cultural importance-index (CI), index of agreement on remedies (IAR) and cultural agreement-index (CAI). Anti-phospholipaseA2 (PLA2) properties of selected plant extracts were also examined. In addition, the cytotoxicity and genotoxicity of the water extract of the plants showing high FL as well as significant PLA2 inhibitory potential were investigated using Allium cepa root tip assay. Results A total of 41 traditional-formulations (TFs) containing 40 plant species (of 39 genera from 28 families) and 3 animal species were prescribed by the THs. Fabaceae exhibited most number of medicinal plants. Piper nigrum (1.78) and Apis cerana indica and Crossopriza lyoni (both 0.21) exhibited the highest UV among the plants and the animals respectively. Stinging of centipede and dog/cat/hyena bite displayed highest ICF (1.00 each). Among the plants, the maximum RI (0.91) and CI (4.98) values were observed for Aristolochia indica. IAR (1.00) was recorded maximum for Achyranthes aspera, Gloriosa superba, Lycopodium cernuum, Smilax zeylanica and Streblus asper. Maximum CAI value was noted for Piper nigrum (5.5096). Among the animals, Apis cerana indica (0.31) and Crossopriza lyoni (1.52) displayed the highest RI and CI values respectively. Crossopriza lyoni (0.99) and Apis cerana indica (1.3871) exhibited maximum IAR and CAI values respectively. Plants showing higher FL exhibited higher anti-PLA2 activity via selective inhibition of human-group PLA2. In addition, Allium cepa root tip assay has indicated the safety and/or toxicity of the plant parts prescribed by the THs. Root water extracts of Aristolochia indica and Gloriosa superba exhibited significant genotoxicity and cytotoxicity. Conclusions Three western districts of West Bengal is the natural abode for many tribal and non-tribal communities. A noteworthy correlation was established between the plants used against poisonous-bites and their anti-PLA2 activity. A few plant parts used by the THs also exhibited high toxicity. Such alternative medical practices serve as the only option in these underprivileged and backward areas during medical-exigencies.

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Genotoxic effects ofHeracleum sosnowskyiin theAllium cepatest

Cited by 14 publications

References 47 publications

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

An evidence based efficacy and safety assessment of the ethnobiologicals against poisonous and non-poisonous bites used by the tribals of three westernmost districts of West Bengal, India: Anti-phospholipase A2 and genotoxic effects

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