Genotoxic effects of low-dose exposure to pirimicarb studied with accelerator mass spectrometry

Wang, Haifang; Wu, Xiaohong; Li, Xinsong; Liu, Yuanfang; Li, Kun; Lu, Xuesong; Wang, Jianjun; Liu, Kexin

doi:10.1007/bf02882582

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…Other toxins in which DNA adduct formation has been examined include nicotine and nicotine derived nitrosamine in mouse liver [30], the insecticide pirimicarb also in mouse liver [31] and trichloroethylene [32]. All three compounds bound to DNA to a varying degree.…”

Section: Macromolecular Adductsmentioning

confidence: 99%

Current perspectives of 14 C-isotope measurement in biomedical accelerator mass spectrometry

Lappin¹,

Garner²

2004

Analytical and Bioanalytical Chemistry

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Accelerator mass spectrometry (AMS) is an extremely sensitive nuclear physics technique developed in the mid-70's for radiocarbon dating of historical artefacts. The technique centres round the use of a tandem Van de Graaff accelerator to generate the potential energy to permit separation of elemental isotopes at the single atom level. AMS was first used in the early 90's for the analysis of biological samples containing enriched 14C for toxicology and cancer research. Since that time biomedical AMS has been used in the study of (1) metabolism of xenobiotics in animals and humans (2) pathways of drug metabolism (3) biomarkers (4) metabolism of endogenous molecules including vitamins (5) DNA and protein binding studies and (6) clinical diagnosis. A new drug development concept which relies on the ultrasensitivity of AMS known as human microdosing (Phase 0) is being used to obtain early human metabolism information of candidate drugs arising out of discovery. These various aspects of AMS are reviewed in this article and a perspective on future applications of AMS provided.

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Section: Macromolecular Adductsmentioning

confidence: 99%

Current perspectives of 14 C-isotope measurement in biomedical accelerator mass spectrometry

Lappin¹,

Garner²

2004

Analytical and Bioanalytical Chemistry

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Applications of 14C-AMS for Carcinogen, Pharmaceutical and Nutrient Studies

Wang

Liu

Sun

2001

J. Nucl. Radiochem. Sci.

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Accelerator mass spectrometry (AMS) is an ultrasensitive measure for tracing i4C labeled molecules in vivo or detecting the biomarker for assessment of carcinogenesis. Basic principles, wide applications and new progresses of bio-14C-AMS have been presented. It is a state-of-the-art tool for measuring the adduction of biological molecules with xenobiotics, including carcinogens, drugs, agrochemicals, nicotine etc.. We have studied the genotoxicity and proteins adduction of smoking specific nicotine and its nitrosamine derivative NNK since 1994. The successful applications have proven the effectiveness of AMS in assessment of cancer risk, screening of drug toxicity and studies of nutrient uptake. In particular, AMS is characterized by measuring xenobiotics at very low dose levels relevant to human environmental exposure. It is sensitive and precise to an attomole (10-18 mole) of 14C per mg carbon. Although it has some shortcomings, undoubtedly, AMS possesses an evident merit of high sensitivity and will have widespread applications in the biomedical sciences.

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Genotoxic effects of low-dose exposure to pirimicarb studied with accelerator mass spectrometry

Cited by 2 publications

References 6 publications

Current perspectives of 14 C-isotope measurement in biomedical accelerator mass spectrometry

Current perspectives of 14 C-isotope measurement in biomedical accelerator mass spectrometry

Applications of 14C-AMS for Carcinogen, Pharmaceutical and Nutrient Studies

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