Genotoxic effects of silver nanoparticles on a tropical marine amphipod via feeding exposure

Botelho, Marina Tenório; Passos, Maria José de Arruda Rocha Campos; Trevizani, Tailisi Hoppe; Figueira, Rubens César Lopes; Umbuzeiro, Gisela de Aragão; Gomes, Vicente

doi:10.1016/j.mrgentox.2022.503527

Cited by 14 publications

(8 citation statements)

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“…A qualitative assay to verify phagocytosis responses was performed (methods adapted from Bergami et al, 2019, and Botelho et al, 2022) using cultures of Escherichia coli (DH5α). Hemolymph was collected from two individuals and added to 100 μL of reconstituted seawater.…”

Section: Methodsmentioning

confidence: 99%

“…Amphipods are an order of Crustacea widely used in ecotoxicology (Anderson et al, 2004; Ingersoll et al, 2002; X. Wang & Zauke, 2004; Weber et al, 2013). Several studies have used the hemolymph of amphipods to measure adverse effects, such as DNA damage (comet assay), from various types of exposures (Botelho et al, 2022; Lacaze et al, 2010; Rocha et al, 2015; Ronci et al, 2015); however, these studies provide minimal information about the cell types present in the hemolymph, or their proportions. The hemocytes have been briefly described only for Echinogammarus stammeri , a freshwater amphipod (Dezfuli et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Because of the increasing interest in the use of the marine amphipod Parhyale hawaiensis in ecotoxicology (Artal et al, 2018, 2019; Botelho et al, 2022; dos Santos, Botelho, et al, 2022; Dos Santos, Vannuci‐Silva, et al, 2022; Vacchi et al, 2019; Vannuci‐Silva et al, 2018, 2019) and in other sciences (Paris et al, 2022; Rehm et al, 2009), we here describe their hemocyte characteristics and identify the various cell types and their proportions, as well as their ability to phagocytize bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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Hemocytes are circulating blood cells that play a crucial function in amphipods and other crustacean immune systems. The hemocytes of the marine tropical amphipod Parhyale hawaiensis have been used for the evaluation of DNA damage and micronuclei, but they have not been characterized in the scientific literature.The aim of this study was to describe the hemolymph cells of P. hawaiensis and study their phagocytotic activity. Basic dyes were used to differentiate the cell types and the presence of lipids. The total hemocyte counts (THCs) and the proportion and sizes of the hemocyte types were determined. Hemolymph was exposed to Escherichia coli for verification of the presence of phagocytosis. Three cell types, all containing lipids, were identified in P. hawaiensis: granulocytes (oval shape, 13.4 Â 7.6 μm), semi-granulocytes (oval shape, 14.1 Â 7.2 μm), and hyalinocytes (round shape, 9.6 Â 7.2 μm). Those three cell types were found in different percentages in males (64.8%, 31.1%, and 4.2%) and females (70.1%, 28.2%, and 1.7%). THCs for males were 9007 ± 3800 cells per individual and 4695 ± 1892 cells per individual for females. The cells of E. coli were phagocytized by the hemocytes. Our findings increased the knowledge of hemocytes in P. hawaiensis and is a step forward in using hemocyte-based immune responses as an endpoint in ecotoxicology.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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“…Micronuclei frequency was determined in hemocytes of P. hawaiensis adults, according to Botelho et al (2022). Twelve organisms (six males and six females) were exposed to two concentrations of 6PPD quinone (250 and 500 μg/L), solvent control (DMSO 0.01%), and positive control (1.5 mg/L Zn 2+ ) for 96 h at 24 ± 2°C and photoperiod of 12 h light/dark.…”

Section: Methodsmentioning

confidence: 99%

Toxicity and mutagenicity studies of 6PPD‐quinone in a marine invertebrate species and bacteria

Botelho,

Militão,

Brinkmann

et al. 2023

Environ and Mol Mutagen

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N-(1,3-dimethylbutyl)-N 0 -phenyl-p-phenylenediamine-quinone (6PPD-quinone), an oxidation product of the tire additive, 6PPD, has been associated with high mortality of salmonids (0.1 μg/L). The objective of this study was to determine the acute toxicity using neonates and mutagenicity (micronuclei in hemolymph of exposed adults) of 6PPD-quinone in the marine amphipod Parhyale hawaiensis. Also, we studied its mutagenicity in the Salmonella/microsome assay using five strains of Salmonella with and without metabolic system (rat liver S9, 5%). 6PPD-quinone did not present acute toxicity to P. hawaiensis from 31.25 to 500 μg/L. Micronuclei frequency increased after 96 h-exposure to 6PPD-quinone (250 and 500 μg/L) when compared to the negative control. 6PPD-quinone also showed a weak mutagenic effect for TA100 only in the presence of S9. We conclude that 6PPD-quinone is mutagenic to P. hawaiensis and weakly mutagenic to bacteria. Our work provides information for future risk assessment of the presence of 6PPD-quinone in the aquatic environment.

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“…Ademais, foram reportadas alterações significativas da microbiota no solo após aplicação de nanomateriais, o que poderia influenciar negativamente à produção vegetal, uma vez que microrganismos do solo desempenham atividades na nutrição, no crescimento e na proteção das plantas (DOOLETTE et al, 2016;MCGEE et al, 2018). Efeitos de nanomateriais carbônicos e inorgânicos a artrópodes estão mais bem elucidados, com evidências de efeitos neurotóxicos, na inibição da atividade de enzimas antioxidantes, em alterações bioquímicas e estruturais, além de efeitos negativo no desenvolvimento e reprodução (BOTELHO et al, 2022;EL-ASHRAM et al, 2020;EL-SAMAD et al, 2022;SHAHRZAD;MANZOOR, 2019;ZACOUTEGUY et al, 2021). Evidências apontam o risco de biomagnificação de nanopartículas no ambiente (JUDY et al, 2011).…”

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Aplicação de dsRNA sintético ou associado a nanocarreadores de zeína para controle de Tuta absoluta (Meyrick, 1917) por RNAi

Rodrigues

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Genotoxic effects of silver nanoparticles on a tropical marine amphipod via feeding exposure

Cited by 14 publications

References 46 publications

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Toxicity and mutagenicity studies of 6PPD‐quinone in a marine invertebrate species and bacteria

Aplicação de dsRNA sintético ou associado a nanocarreadores de zeína para controle de Tuta absoluta (Meyrick, 1917) por RNAi

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