Genotoxic Effects of the Alkaloids Harman and Harmine Assessed by Comet Assay and Chromosome Aberration Test in Mammalian Cells <i>in vitro</i>

Boeira, Jane Marlei; Silva, Juliana da; Erdtmann, Bernardo; Henriques, João Antônio Pêgas

doi:10.1034/j.1600-0773.2001.d01-162.x

Cited by 72 publications

(39 citation statements)

References 40 publications

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“…The Tukey test was applied for post-hoc comparison. A cytotoxic response was considered when the viability values observed were below 70%, when compared to the control (Boeira et al, 2001;Cardozo et al, 2006;Žegura et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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Some water bodies in the Sinos River Basin (SRB) have been suffering the effects of pollution by residential, industrial and agroindustrial wastewater. The presence of cytotoxic and genotoxic compounds could compromise the water quality and the balance of these ecosystems. In this context, the research aimed to evaluate the genotoxicity and cytotoxicity of the water at four sites along the SRB (in the cities of Santo Antônio da Patrulha, Parobé, Campo Bom and Esteio), using bioassays in fish and cell culture. Samples of surface water were collected and evaluated in vitro using the Astyanax jacuhiensis fish species (micronucleus test and comet assay) and the Vero lineage of cells (comet assay and cytotoxicity tests, neutral red -NR and tetrazolium MTT). The micronucleus test in fish showed no significant differences between the sampling sites, and neither did the comet assay and the MTT and NR tests in Vero cells. The comet assay showed an increase in genetic damage in the fish exposed to water samples collected in the middle and lower sections of the basin (Parobé, Campo Bom and Esteio) when compared to the upper section of the basin (Santo Antônio da Patrulha). The results indicate contamination by genotoxic substances starting in the middle section of the SRB.Keywords: water quality, micronucleus test, comet assay, fish bioassays, cell culture. Avaliação da genotoxicidade e da citotoxicidade de amostras de água da Bacia do Rio dos Sinos, sul do Brasil ResumoAlguns corpos d'água da Bacia Hidrográfica do Rio dos Sinos (BHRS) vêm sofrendo os efeitos da poluição por efluentes domésticos, industriais e agroindustriais. A presença de compostos citotóxicos e genotóxicos pode comprometer a qualidade da água e o equilíbrio desses ecossistemas. Neste contexto, o objetivo do trabalho foi avaliar a genotoxicidade e a citotoxicidade da água em quatro pontos ao longo da BHRS (Santo Antônio da Patrulha, Parobé, Campo Bom e Esteio), utilizando bioensaios em peixes e em cultura celular. As amostras de água de superfície foram coletadas e avaliadas in vitro utilizando a espécie de peixe Astyanax jacuhiensis (teste de micronúcleo e ensaio cometa) e a linhagem celular tipo Vero (ensaio cometa e os testes de citotoxicidade vermelho neutro -VN e tetrazólio MTT). O teste de micronúcleos em peixes não apresentou diferenças significativas entre os pontos de coleta, assim como o ensaio cometa e os testes VN e MTT nas células Vero. O ensaio cometa demonstrou aumento nos danos genéticos em peixes expostos às amostras de água coletadas nos trechos médio e inferior da bacia (Parobé, Campo Bom e Esteio) em relação ao trecho superior da bacia (Santo Antônio da Patrulha). Os resultados indicam contaminação por substâncias genotóxicas a partir do trecho médio da BHRS.Palavras-chave: qualidade da água, teste de micronúcleo, ensaio cometa, bioensaios com peixes, cultura celular.

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Section: Discussionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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“…Cytotoxic and genotoxic effects of harmine were investigated in V79 Chinese hamster lung fibroblasts in vitro using singlecell gel assay, also known as Comet assay, and the results showed that harmine increased aberrant cell frequency and induced DNA damage as evidenced by the Comet assay [35] . Effects of harmine on yeast Saccharomyces cerevisiae were investigated to verify putative genotoxicity, mutagenicity and recombinogenicity.…”

Section: Effect Of Harmine Against Cancermentioning

confidence: 99%

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Patel

Gadewar

Tripathi

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

219

120

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“…The colonies were fixed with methanol-acetic acid (3:1) and stained with 0.1% violet crystal. Any sample that presented a number of colonies equal to or less than 70% of the number of colonies in the negative control was considered cytotoxic (Speit et al 1998;Boeira 2001).…”

Section: Methodsmentioning

confidence: 99%

Ecotoxicological Evaluation in an Effluent and Petrochemical Waste Disposal Area

Terra

Feiden

Lemos

et al. 2009

Water Air Soil Pollut

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Water and sediment were studied to assess the impact of wastes from an area used for a disposal area of treated petrochemical effluents in Rio Grande do Sul, Brazil. The study was performed using the Daphnia magna (Straus 1820) for chronical evaluations, mutagenesis in Salmonella/microsome assays, and micronuclei induction in cultures of V79 cell to assess genotoxicity. Six sites were defined for chronic and genotoxic tests by micronuclei induction with liquid and sediment samples. Long-term tests were planned in semi-static flow, with microcrustaceans 2 to 26 h old in the beginning of assays. The minimum level of reproduction required to maintain the species was not reached. There are delays for the beginning of the reproductive process. Survival was also affected in some samples. The reproductive responses were more sensitive on identifying environmental quality than the survival rate. The study of mutagenicity by Salmonella/microsome assay made it possible to define the seasonality of the components showing greater frequency in winter. The predominant event was the frameshift mutation in assays with the presence of metabolism. However, the cytotoxic activity, although present in all seasons, was less frequent in winter. The genotoxicity analysis in V79 cells exposed to liquid samples from the area also showed that cytotoxicity was the most frequent event. This may have interfered in the detection of a potential micronuclei induction. The results showed that, even after treatment, effluents disposed on the soil continue with active pollutants interfering in cladoceran's quality of life, cellular physiology, and DNA integrity.

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Genotoxic Effects of the Alkaloids Harman and Harmine Assessed by Comet Assay and Chromosome Aberration Test in Mammalian Cells in vitro

Cited by 72 publications

References 40 publications

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Ecotoxicological Evaluation in an Effluent and Petrochemical Waste Disposal Area

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