Genotoxic effects of vinclozolin on the aquatic insect Chironomus riparius (Diptera, Chironomidae)

Aquilino, Mónica; Sánchez-Argüello, Paloma; Martínez-Guitarte, José-Luis

doi:10.1016/j.envpol.2017.09.088

Cited by 14 publications

(6 citation statements)

References 45 publications

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“…Taken together, this may indicate that the sGO nanomaterial would be less damaging to the cell compared to mlGO and lGO, since it does not cause apoptosis. Similar results for NLK gene and DECAY inactivation have been obtained previously in C. riparius after treatment to the fungicide Vinclozolin [ 36 ]. In addition, the results obtained with mlGO and lGO could suggest that the activation of the repair mechanisms produced after their exposure in the C. riparius larvae would not be sufficient to stop cell apoptosis.…”

Section: Resultssupporting

confidence: 87%

“…In this work, we studied the transcriptional activity of XRCC1 , ATM , NLK and DECAY genes, whose actions are related to the genotoxic effects derived from exposure to different compounds. These genes have been previously characterized and analyzed in C. riparius [ 36 ]. They are involved in different DNA repair mechanisms and the resulting expression pattern demonstrates a variety of damage.…”

Section: Resultsmentioning

confidence: 99%

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Graphene Oxides (GOs) with Different Lateral Dimensions and Thicknesses Affect the Molecular Response in Chironomus riparius

et al. 2023

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Graphene oxide (GO) materials possess physicochemical properties that facilitate their application in the industrial and medical sectors. The use of graphene may pose a threat to biota, especially aquatic life. In addition, the properties of nanomaterials can differentially affect cell and molecular responses. Therefore, it is essential to study and define the possible genotoxicity of GO materials to aquatic organisms and their ecosystems. In this study, we investigated the changes in the expression of 11 genes in the aquatic organism Chironomus riparius after 96 h of exposure to small GOs (sGO), large GOs (lGO) and monolayer GOs (mlGO) at 50, 500 and 3000 μg/L. Results showed that the different genes encoding heat shock proteins (hsp90, hsp70 and hsp27) were overexpressed after exposure to these nanomaterials. In addition, ATM and NLK—the genes involved in DNA repair mechanisms—were altered at the transcriptional level. DECAY, an apoptotic caspase, was only activated by larger size GO materials, mlGO and lGO. Finally, the gene encoding manganese superoxide dismutase (MnSOD) showed higher expression in the mlG O-treated larvae. The lGO and mlGO treatments indicated high mRNA levels of a developmental gene (FKBP39) and an endocrine pathway-related gene (DRONC). These two genes were only activated by the larger GO materials. The results indicate that larger and thicker GO nanomaterials alter the transcription of genes involved in cellular stress, oxidative stress, DNA damage, apoptosis, endocrine and development in C. riparius. This shows that various cellular processes are modified and affected, providing some of the first evidence for the action mechanisms of GOs in invertebrates. In short, the alterations produced by graphene materials should be further studied to evaluate their effect on the biota to show a more realistic scenario of what is happening at the molecular level.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Graphene Oxides (GOs) with Different Lateral Dimensions and Thicknesses Affect the Molecular Response in Chironomus riparius

et al. 2023

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“…In particular, it is the most frequently fungicide used by the wine industry [ 10 ]. Many papers in the literature indicate vinclozolin as an environmental and aquatic pollutant [ 11 , 12 , 13 ]. Vinclozolin dissipates in the environment by microbial-mediated hydrolysis, soil metabolism, abiotic degradation, and transport by water.…”

Section: Introductionmentioning

confidence: 99%

Chronic Exposure to Vinclozolin Induced Fibrosis, Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis in Mice Kidney

Paola

D’Amico

Genovese

et al. 2022

IJMS

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Vinclozolin is one of the most used fungicides in the control of fungi in fruits, vegetables, and ornamental plants. The effects of its exposure on different organs have been described, but information regarding its relevance to vinclozolin-induced nephrotoxicity is largely missing. This study focuses on the potential mechanism of vinclozolin-induced nephrotoxicity. CD1 male mice were administered vinclozolin (100 mg/kg) by oral gavage for 28 days. Vinclozolin administration decreased body weight over the treatment period and at the end of the experiment, increased the ratio of kidney weight to body weight and increased serum urea nitrogen and creatinine contents. Vinclozolin also induced histopathological alterations, including tubular dilatation and necrosis and impaired the integrity of the renal-tubular architecture and kidney fibrosis. The analyses conducted showed that vinclozolin administration altered the mRNA levels of mitochondrial function-related proteins (SIRT3, SIRT1, PGC-1α, TFAM, NRF1, VDAC-1, and Cyt c) and oxidative stress (increased lipid peroxidation and decreased total antioxidative capacity, catalase, and superoxide dismutase activities, glutathione levels, and glutathione peroxidase activity) in the kidneys. Furthermore, vinclozolin induced toxicity that altered Nrf2 signalling and the related proteins (HO-1 and NQO-1). Vinclozolin administration also affected both the extrinsic and intrinsic apoptotic pathways, upregulating the expression of proapoptotic factors (Bax, Caspase 3, and FasL) and downregulating antiapoptotic factor (Bcl-2) levels. This study suggests that vinclozolin induced nephrotoxicity by disrupting the transcription of mitochondrial function-related factors, the Nrf2 signalling pathway, and the extrinsic and intrinsic apoptotic pathways.

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“…Following this study, D’Amico et al demonstrated that exposure to vinclozolin (and other endocrine disruptors) significantly induced oxidative stress and inflammation, worsening arthritis and Parkinson’s disease in experimental murine models [ 9 , 10 ]. In consideration of the fact that most previous studies investigated the effects of vinclozolin exposure on the reproductive system, we investigated for the first time the impact of long exposure to vinclozolin on the respiratory system [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

D’Amico

Paola

Impellizzeri

et al. 2022

IJMS

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Endocrine-disrupting substances (EDS) are common and pervasive in our environment and pose a serious risk to both human and animal health. Endocrine-disrupting compounds (EDCs) have been associated with a variety of detrimental human health effects, including respiratory issues, as a result of their ability to disrupt cell physiology. Vinclozolin ((RS)-3-(3,5-Dichlorophenyl)-5-methyl-5-vinyloxazolidine-2,4-dione) is a common dicarboximide fungicide used to treat plant diseases. Several studies have analyzed the effects of vinclozolin exposure on the reproductive system, but less is known about its effect on other organs such as the lung. Mice were exposed for 28 days to orally administered vinclozolin at a dose of 100 mg/kg. Vinclozolin exposure induced histological alterations and collagen depositions in the lung. Additionally, vinclozolin induced inflammation and oxidative stress that led to lung apoptosis. Our study demonstrates for the first time that the toxicological effects of vinclozolin are not limited to the reproductive system but also involve other organs such as the lung.

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Genotoxic effects of vinclozolin on the aquatic insect Chironomus riparius (Diptera, Chironomidae)

Cited by 14 publications

References 45 publications

Graphene Oxides (GOs) with Different Lateral Dimensions and Thicknesses Affect the Molecular Response in Chironomus riparius

Graphene Oxides (GOs) with Different Lateral Dimensions and Thicknesses Affect the Molecular Response in Chironomus riparius

Chronic Exposure to Vinclozolin Induced Fibrosis, Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis in Mice Kidney

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

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