Genotoxic evaluation of occupational exposure to antineoplastic drugs

Aristizábal-Pachón, Andrés Felipe; Castillo, Willian Orlando

doi:10.1007/s43188-019-00003-7

Cited by 15 publications

(9 citation statements)

References 38 publications

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“…Several previous studies have assessed the effect of antineoplastic drug exposure on DNA damage by comet assay and CBMN assay with varying conclusions. Similar to our results, some studies from different countries have found a significant increase of DNA damage in subjects exposed to antineoplastic drugs as compared with control subjects 21–25. Moreover, a recent systematic review and meta-analysis demonstrated a clear and significant association between occupational exposure to antineoplastic drugs and the extent of primary DNA damage in health professionals 7.…”

Section: Discussionsupporting

confidence: 91%

Effect of occupational exposure to antineoplastic drugs on DNA damage in nurses: a cross-sectional study

Huang

Gao²,

Cai

et al. 2021

Occup Environ Med

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BackgroundAlthough the therapeutic effect of antineoplastic drugs is incontestable, these agents can also potentially act as carcinogens, mutagens and/or teratogens in people. The aim of this study was to assess the effect of occupational exposure to antineoplastic drugs on DNA damage, assessed by the comet assay and cytokinesis-block micronucleus (CBMN) assay, in nurses.MethodsThe cross-sectional study enrolled 305 nursing staff members from 7 public hospitals in Shenzhen who handled antineoplastic drugs, and 150 healthy nursing staff members who were not exposed to antineoplastic drugs as the control group. DNA damage was assessed by the comet and CBMN assay. Multiple linear regressions and logistic regressions models were used to analyse the effect of occupational exposure to antineoplastic drugs on DNA damage.ResultsAfter adjustment for confounding factors, compared with non-exposure to antineoplastic drugs, exposure to antineoplastic drugs was positively related to tail moment, olive moment, tail length and tail DNA per cent, and adjusted β or OR (95% CI) was 0.17 (0.08 to 0.26), 0.18 (0.10 to 0.27), 1.03 (0.47 to 1.60) and 1.16 (1.04 to 1.29) (all p<0.05). Moreover, similar significant relationships were observed for the biomarkers of the CBMN assay. Additionally, other than age, there was no interaction between antineoplastic drug exposure and other variables for the levels of biomarkers of the CBMN assay and the comet assay.ConclusionsThe present results showed that exposure to antineoplastic drugs was positively related to the risk of DNA damage in nurses. The results imply that occupational exposure to antineoplastic agents is an important global public health problem that requires urgent attention.

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Section: Discussionsupporting

confidence: 91%

Effect of occupational exposure to antineoplastic drugs on DNA damage in nurses: a cross-sectional study

Huang

Gao²,

Cai

et al. 2021

Occup Environ Med

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“…From the 21 articles read in full, 2 were excluded because they did not present comet assay data. Nineteen studies of occupational exposure [ 12 , 16 , 191 , 196 , 200 , 201 , 202 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 ] remained for qualitative analysis, as summarised in Figure 3 and Table 3 .…”

Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

Ladeira,

Møller,

Giovannelli

et al. 2024

Toxics

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Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important tool for assessing DNA damage, both in environmental and occupational exposure contexts. To evidence the role of the comet assay in human biomonitoring, we have analysed original research studies of environmental or occupational exposure that used the comet assay in their assessments, following the PRISMA-ScR method (preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews). Groups of chemicals were designated according to a broad classification, and the results obtained from over 300 original studies (n = 123 on air pollutants, n = 14 on anaesthetics, n = 18 on antineoplastic drugs, n = 57 on heavy metals, n = 59 on pesticides, and n = 49 on solvents) showed overall higher values of DNA strand breaks in the exposed subjects in comparison with the unexposed. In summary, our systematic scoping review strengthens the relevance of the use of the comet assay in assessing DNA damage in human biomonitoring studies.

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“…2,4 The prevalence of micronuclei (DNA fragments) in epithelial cells and peripheral lymphocytes of healthcare workers exposed to cytotoxic drugs was also found to be statistically higher compared to controls. 3,5 There is also evidence to suggest that oxidative stress is increased in exposed professionals. 6 The International Society of Oncology Pharmacy Practitioners, 7 the Occupational Safety and Health Administration (OSHA) 8 and the American Society of Health-System Pharmacists 9 recommend that employers mandatorily ensure training before starting handling these drugs.…”

Section: Introductionmentioning

confidence: 99%

A simple and inexpensive method to monitor and minimize exposure from manipulation of cytotoxic drugs

Ali

Carmo

Robalo

et al. 2023

J Oncol Pharm Pract

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Pharmacy personnel that manipulate cytotoxic drugs are under continuous exposure risk. Therefore, training and strict adherence to recommended practices should always be promoted. The main objective of this study was to develop and apply a safe, effective and low-cost method for the training and assessment of the safe handling of cytotoxic drugs, using commercially available tonic water. To evaluate the potential of tonic water as a replacement marker for quinine hydrochloride, deliberate spills of 1 mL of four different tonic waters (one coloured and three non-coloured) were analysed under ultraviolet light (300–400 nm). The pigmented sample did not produce fluorescence under ultraviolet (UV) light. The three commercially available tonic waters that exhibited fluorescence were further analysed by UV/Vis spectrophotometry (300–500 nm). Afterwards, a protocol of simulated manipulation of cytotoxic drugs was developed and applied to 12 pharmacy technicians, that prepared 24 intravenous bags according to recommended routine procedures using tonic water. Participants responded to a brief questionnaire to evaluate the adequacy and applicability of the activity. Seven of the participants had spillages during manipulation, the majority of which recorded during manipulation with needles. All participants scored the tonic water manipulation simulation with 4 or 5 points for simplicity, efficiency and feasibility. The obtained results suggest that tonic water can be used to simulate the manipulation of cytotoxic drugs in training and assessment programs. By using this replacement marker for quinine hydrochloride, it is possible to perform a more cost-effective, yet equally effective, assessment.

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Genotoxic evaluation of occupational exposure to antineoplastic drugs

Cited by 15 publications

References 38 publications

Effect of occupational exposure to antineoplastic drugs on DNA damage in nurses: a cross-sectional study

Effect of occupational exposure to antineoplastic drugs on DNA damage in nurses: a cross-sectional study

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

A simple and inexpensive method to monitor and minimize exposure from manipulation of cytotoxic drugs

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