Genotoxic potential of ethinylestradiol in cultured mammalian cells

Siddique, Yasir Hasan; Beg, Tanveer; Afzal, Mohammad

doi:10.1016/j.cbi.2004.10.008

Cited by 27 publications

(15 citation statements)

References 47 publications

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“…Catalase is found to reduce sister chromatid exchange levels resulting from the treatment of hydrogen peroxide by breaking hydrogen peroxide into water and oxygen (Culotta, 2000). Similar results were obtained in our earlier study performed on ethinylestradiol (Siddique et al, 2005a). Since the medroxyprogesterone acetate is genotoxic only in the presence of S9 mix with NADP, the first step would involve, the aromatic hydroxylation catalysed by cytochrome P450s (Fig.…”

Section: Discussionsupporting

confidence: 80%

Genotoxic potential of medroxyprogesterone acetate in cultured human peripheral blood lymphocytes

et al. 2006

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Section: Discussionsupporting

confidence: 80%

Genotoxic potential of medroxyprogesterone acetate in cultured human peripheral blood lymphocytes

et al. 2006

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“…There are several reports on the genotoxic potential of estrogens and synthetic progestins in vivo and in vitro [21][22][23][24][25][26][27][28]. Most of the oral formulations have a definite proportion of estrogen and synthetic progesterone [29]. However, the composition of the OCs can vary from country to country so as the response toward the drugs forms the basis of pharmacogenomics [30].…”

Section: Discussionmentioning

confidence: 99%

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

et al. 2015

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The continuous use of synthetic hormones as contraceptive pill or hormonal replacement therapy among women is increasing day by day. The widespread use of different formulations as oral contraceptives by women throughout their reproductive cycle has given rise to a serious concern for studying the effects of oral contraceptives on enzymatic profile and DNA damage in peripheral blood lymphocytes among users. The present study was carried out on women taking oral contraceptives. The study was based on the questionnaire having the information of reproductive history, fasting, age, health, nature of menstrual cycle, bleeding and other disease. The profile of the blood serum enzymes i.e. alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), lactate dehydrogenase (LDH), aminotransferases (SGOT and SGPT), serum proteins (albumin and globulin) and DNA damage in lymphocytes was studied among users and non-users. The results of the present study suggest that OCs not only effects enzymatic activity but also results in DNA damage that may vary with the duration of using oral contraceptives. A significant increase in LDH, GGT, SGPT, SGOT, globulin and decrease in ALP as well as albumin was found among users as compared to non-users. The observed DNA damage was more in users as compared to non-users. Hormonal contraceptives seem to exert DNA damage and also have significant effects on blood serum enzymes.

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“…This may be because of presence of more number of Group C chromosomes in normal karyotype. Siddique et al observed the genotoxic effect of steroid at higher doses [9,16,17]. The authors also concluded that therapeutic doses are safe, but they may be even genotoxic in long term usage [18,19].…”

Section: Resultsmentioning

confidence: 98%

“…Ahmed M E et al studied genotoxicity of norethindrone(norethisterone) and norgestrel, lymphocytes were exposed to three different concentrations of the drug 20, 40 and 75 ìg /ml for norethindrone [16]. Norgestrel was studied at 10, 25, 50 ìg /ml concentrations.…”

Section: Resultsmentioning

confidence: 99%

Effect of Synthetic Progesterone (Norethisterone) on Human Chromosomes: A Cytogenetic Study From India

Sumedha¹,

S.D²

2018

IJAR

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Birth restriction methods dates back to prehistoric times, half a million years ago. Modern contraceptive methods constitute most contraceptive use. Nearly 800 million married or in-union women are projected to be using contraception in 2030. Norethisterone (synthetic progesterone) is used for many therapeutic purposes, and is being used by millions of women in India. The present study was carried out during August 2009 to July 2011 on sixty fertile females within reproductive age group. Chromosomal analysis was carried out to find the effects of synthetic progesterone (Norethisterone) on human chromosomes in lymphocyte culture in vitro in three groups at 0, 75 µg, 100 µg of drug per ml respectively and observed for chromosomal aberrations like break, gap, dicentric chromosome and chromosomal association. Chromosomal aberrations were significantly increased at higher concentrations. Mean chromosomal gaps at 0µg/ml, 75µg/ml and 100 µg/ml concentration were 6.90, 7.62 and 10.58 respectively and mean chromosomal breaks in that same concentration were 6.63, 7.28 and 10.08 respectively. 30 samples of the 60 showed chromosomal associations and 5 showed dicentric chromosomes. There is a direct correlation between increase in concentration of Norethisterone and structural chromosomal aberrations, which may be carried to next generation, and lead to anomalies in progeny of woman taking such high doses of synthetic progesterone.

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Genotoxic potential of ethinylestradiol in cultured mammalian cells

Cited by 27 publications

References 47 publications

Genotoxic potential of medroxyprogesterone acetate in cultured human peripheral blood lymphocytes

Genotoxic potential of medroxyprogesterone acetate in cultured human peripheral blood lymphocytes

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

Effect of Synthetic Progesterone (Norethisterone) on Human Chromosomes: A Cytogenetic Study From India

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