Genotoxic properties of Betonica officinalis, Gratiola officinalis, Vincetoxicum luteum and Vincetoxicum hirundinaria extracts

Slapšytė, G.; Dedonyte, Veronika; Adomėnienė, Aušra; Lazutka, Juozas R.; Kazlauskaitė, Jūratė; Ragažinskienė, Ona; Venskutonis, Petras Rimantas

doi:10.1016/j.fct.2019.110815

Cited by 11 publications

(6 citation statements)

References 40 publications

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“…Another research group has performed genotoxicity evaluations demonstrating that Betonica officinalis extract does not induce chromosome aberrations in normal human lymphocytes in vitro. However, DNA damage was detected and a slight increase in sister chromatid exchange values, which was attributed to the plant extract and potential donor susceptibility [23]. Tumor cells were not included in the study in order to determine stronger genotoxic effect against these type of cells.…”

Section: Discussionmentioning

confidence: 99%

Antitumor and Immunomodulatory Properties of the Bulgarian Endemic Plant Betonica bulgarica Degen et Neič. (Lamiaceae)

Mladenova

Batsalova

Dzhambazov

et al. 2022

Plants

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Extracts obtained from different Betonica species have been shown to possess important biological properties. The present study aimed to investigate the cytotoxicity, antitumor and immunomodulatory potential of the endemic plant Betonica bulgarica (Lamiaceae) and thus, reveal new aspects of its biological activity. Methods: Methanolic extract obtained from inflorescences was analyzed for cytotoxicity against mammalian cell lines. The antitumor potential of the sample was determined using human cervical and lung adenocarcinoma cells (HeLa and A549). Programmed cell death-inducing effects against HeLa cells and peripheral blood lymphocytes, as well as immunomodulatory properties of the extract were determined by flow cytometry analysis. Results: The research results demonstrated that the extract has significant inhibitory potential against HeLa cells (mean IC50 value 119.2 μg/mL). The sample selectively induced apoptotic death in tumor cells. Cytotoxic effects towards mouse cell lines were detected following treatment with high concentrations of Betonica bulgarica extract (200 and 250 μg/mL). Twenty-four-hour ex vivo incubation of peripheral blood leucocytes in growth medium containing plant extract induced prominent effects in distinct immune cell populations. They included elevated levels of CD25+ and CD56+ T cells’ lymphocytes, particularly CD4+CD25+ and CD8+CD56+ cells. Conclusions: The present study demonstrates that Betonica bulgarica inflorescence extract possesses potential beneficial antitumor and immunomodulatory activity and could serve as a source of bioactive compounds with biomedical application.

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Section: Discussionmentioning

confidence: 99%

Antitumor and Immunomodulatory Properties of the Bulgarian Endemic Plant Betonica bulgarica Degen et Neič. (Lamiaceae)

Mladenova

Batsalova

Dzhambazov

et al. 2022

Plants

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“…The most accepted SCE formation mechanism is based on a double-strand break that occurs during DNA replication. Some environmental agents can cause a pathologic homologous recombination between the sister chromatids of chromosomes [48]. It is essential to evaluate if magnetite nanoparticles or M-CS/Tween 80 microspheres can cause this kind of exchange between genetic material because some changes in the chromosome sequence can cause several pathologies such as cancer.…”

Section: Sister Chromatid Exchangementioning

confidence: 99%

A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

et al. 2021

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Microspheres have been proposed for different medical applications, such as the delivery of therapeutic proteins. The first step, before evaluating the functionality of a protein delivery system, is to evaluate their biological safety. In this work, we developed chitosan/Tween 80 microspheres loaded with magnetite nanoparticles and evaluated cell damage. The formation and physical–chemical properties of the microspheres were determined by FT-IR, Raman, thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), and SEM. Cell damage was evaluated by a full set of in vitro assays using a non-cancerous cell line, human erythrocytes, and human lymphocytes. At the same time, to know if these microspheres can load proteins over their surface, bovine serum albumin (BSA) immobilization was measured. Results showed 7 nm magnetite nanoparticles loaded into chitosan/Tween 80 microspheres with average sizes of 1.431 µm. At concentrations from 1 to 100 µg/mL, there was no evidence of changes in mitochondrial metabolism, cell morphology, membrane rupture, cell cycle, nor sister chromatid exchange formation. For each microgram of microspheres 1.8 µg of BSA was immobilized. The result provides the fundamental understanding of the in vitro biological behavior, and safety, of developed microspheres. Additionally, this set of assays can be helpful for researchers to evaluate different nano and microparticles.

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“…10 Ayrıca homeopatik preparat Engystol'ün bileşimine katılmaktadır. 11 V. canescens subsp. canescens (Willd.)…”

Section: Introductionunclassified

Investigation on Antimicrobial, Antibiofilm Activities; and Synergistic Effects of Seeds of Two Vincetoxicum Taxa Growing in Turkey

Öksüz

Güzel

2022

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi

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Amaç: Bu çalışmanın amacı bazı türleri geleneksel olarak nevroz, sıtma, skrofula, uyuz, ateş, eksternal kanserler, incinme ve yara tedavisinde kullanılan Vincetoxicum cinsine ait iki taksonun (Vincetoxicum canescens subsp. pedunculata (VC) ve Vincetoxicum fuscatum subsp. fuscatum (VF)) tohumlarının antimikrobiyal, antibiyofilm aktiviteleri ve sinerjik etkilerini incelemektir.Yöntem: Öğütülmüş tohumlardan elde edilmiş etanollü ekstrelerin antimikrobiyal etkinlikleri mikrodilüsyon yöntemiyle 5 referans bakteri suşu (Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae) ve 2 referans maya suşuna (Candida albicans and Candida parapsilosis) karşı test edilmiştir. Ayrıca ekstrelerin P. aeruginosa’nın biyofilm oluşumunu inhibe etme ve oluşmuş biyofilm üzerine etkisi kristal viyole yöntemi ile değerlendirilmiştir. Biyofilm testleri ile minimum biyofilm inhibisyon konsantrasyonu (MBIC50) ve minimum biyofilm azaltma konsantrasyonu (MBRC50) belirlenmiştir. E.coli'ye karşı iki ekstre arasındaki sinerjik etkiyi değerlendirmek için ise mikrodilüsyon dama tahtası yöntemi kullanılmıştır.Bulgu: Ekstreler, test edilen bakteri ve mayaları 62,5–250 µg/mL minimum inhibisyon konsantrasyonları (MİK) aralığında inhibe etmiştir. VF ve VC ekstrelerinin 0,5X ve 0,25X MİK'de biyofilm oluşumunu sırasıyla %47, %39 ve %50, %34 oranında inhibe ettiği ve iki ekstrenin MBIC50 değerinin 62,5 µg/mL olduğu belirlenmiştir. Ayrıca ekstrelerin önceden oluşmuş biyofilmi 1X ve 2X MİK'de sırasıyla %54, %62 ve %56, %61 oranında inhibe ettiği ve MBRC50 değerinin 125 µg/mL olduğu belirlenmiştir. Dahası ekstrelerin E.coli'ye karşı aditif etki (FİK=0,62) gösterdikleri de tespit edilmiştir.Sonuç: Test edilen ekstreler orta ve düşük antimikrobiyal etki göstermekle birlikte hem biyofilm oluşumunu engelleme hem de oluşmuş biyofilmi ortadan kaldırmada iyi etkinliğe sahiptir.

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Genotoxic properties of Betonica officinalis, Gratiola officinalis, Vincetoxicum luteum and Vincetoxicum hirundinaria extracts

Cited by 11 publications

References 40 publications

Antitumor and Immunomodulatory Properties of the Bulgarian Endemic Plant Betonica bulgarica Degen et Neič. (Lamiaceae)

Antitumor and Immunomodulatory Properties of the Bulgarian Endemic Plant Betonica bulgarica Degen et Neič. (Lamiaceae)

A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

Investigation on Antimicrobial, Antibiofilm Activities; and Synergistic Effects of Seeds of Two Vincetoxicum Taxa Growing in Turkey

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