2024
DOI: 10.3390/atmos15121420
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Genotoxicity and Cytotoxicity Induced In Vitro by Airborne Particulate Matter (PM2.5) from an Open-Cast Coal Mining Area

Claudia Galeano-Páez,
Hugo Brango,
Karina Pastor-Sierra
et al.

Abstract: This study evaluates the cytotoxic and genotoxic effects of PM2.5 collected from an open-cast coal mining area in northern Colombia. Cyclohexane (CH), dichloromethane (DCM), and acetone (ACE) extracts were obtained using Soxhlet extraction to isolate compounds of different polarities. Human lymphocytes were exposed to the extracted compounds, and cytotoxicity and genotoxicity were assessed using the cytokinesis block micronucleus (CBMN) and comet assays, incorporating FPG and ENDO III enzymes to detect oxidati… Show more

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