Genotoxicity and Cytotoxicity of Zinc Oxide and Titanium Dioxide in HEp-2 Cells

Osman, Ilham F; Baumgartner, Andreas; Cemeli, Eduardo; Fletcher, J.; Anderson, Diana

doi:10.2217/nnm.10.52

Cited by 143 publications

(65 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, the in vitro results obtained using the comet assay in this study produced positive responses independent of the cell type used. This occurred when this nanomaterial was tested using different mammalian cell line models such as primary mouse embryo fibroblasts (Yang et al, 2009), human epidermal (A431) cells (Sharma et al, 2009), human negroid cervix carcinoma Hep-2 cells (Osman et al, 2010), human bronchial epithelial cells BEAS-2B (Ng et al, 2011), human epidermal keratinocytes (HEK) (Sharma et al, 2011a), human liver HepG2 cells (Sharma et al, 2011b), human hepatocytes (L02), and human embryonic kidney (HEK293) cells (Guan et al, 2012). On the other hand, Kumari et al (2011) studied the cytotoxicity and genotoxicity of ZnO NPs (<100 nm) on root cells of A. cepa.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay

Demir¹,

Kaya²,

Kaya³

2014

Turk J Biol

View full text Add to dashboard Cite

Nanogenotoxicity is an emergent field, relevant for estimating the potential genotoxic risk of nanomaterials. In this study we investigated the genotoxic potential of zinc oxide (ZnO, ≤35 and 50 nm) and titanium dioxide (TiO 2 , 21 and 50 nm) nanoparticles (NPs) in the nuclei of Allium cepa root meristem cells by using a modified alkaline comet assay. ZnO and TiO 2 NPs were supplied to cells treated in vitro at 3 different concentrations (10, 100, and 1000 µg/mL). For the comet assay, our results indicate a dose-dependent increment in DNA damage in cells exposed to 100 and 1000 µg/mL of ZnO NPs (≤35 and 50 nm) for percentage of DNA tail (% DNA tail). On the other hand, the results from TiO 2 NPs indicated that the DNA damage in the cells exposed to only the highest concentration (1000 µg/mL) of TiO 2 NPs (21 nm) is statistically significant in comparison to the control cells, while all concentrations of TiO 2 NPs (50 nm) are stat

show abstract

Section: Discussionmentioning

confidence: 99%

Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay

Demir¹,

Kaya²,

Kaya³

2014

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…Zinc nanoparticles in the range 10-100 μg/mL were used for 24-48 h on HEp-2 cells. The observed results from the study demonstrated that the zinc oxide nanoparticles were toxic for the cells causing DNA damage and reduction in cell viability [216]. Another study focused on the assessment of in vivo toxicity of zinc nanoparticles at 14-20 μg/mL for 12 h to determine the effects of treatment on cell viability, DNA damage, ROS production and apoptosis [217].…”

Section: Mechanistic Studiesmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

View full text Add to dashboard Cite

Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

show abstract

“…60 ZnO NPs (,100 nm) induced genotoxicity in HEp-2 human cervix carcinoma cells, using the Comet assay and the cytokinesis-blocked micronucleus assay. 61 Genotoxicity was associated with increased tyrosine phosphorylation.…”

Section: Genotoxicity Studies In Vivo and In Vitro In Vivo Studiesmentioning

confidence: 99%

A review of mammalian toxicity of ZnO nanoparticles

Vandebriel¹,

Jong²

2012

NSA

442

247

View full text Add to dashboard Cite

This review summarizes the literature on mammalian toxicity of ZnO nanoparticles (NPs) published between 2009 and 2011. The toxic effects of ZnO NPs are due to the compound's solubility. Whether the increased intracellular [Zn 2+ ] is due to the NPs being taken up by cells or to NP dissolution in medium is still unclear. In vivo airway exposure poses an important hazard. Inhalation or instillation of the NPs results in lung inflammation and systemic toxicity. Reactive oxygen species (ROS) generation likely plays an important role in the inflammatory response. The NPs do not, or only to a minimal extent, cross the skin; this also holds for sunburned skin. Intraperitoneal administration induces neurological effects. The NPs show systemic distribution; target organs are liver, spleen, lung, and kidney and, in some cases, the heart. In vitro exposure of BEAS-2B bronchial epithelial cells and A549 alveolar adenocarcinoma cells results in cytotoxicity, increased oxidative stress, increased intracellular [Ca 2+ ], decreased mitochondrial membrane potential, and interleukin (IL)-8 production. Decreased contractility of airway smooth muscle cells poses an additional hazard. In contrast to the results for BEAS-2B and A549 cells, in RKO colon carcinoma cells ZnO NPs and not Zn 2+ induce cytotoxicity and mitochondrial dysfunction. Short-term exposure of skin cells results in apoptosis but not in an inflammatory response, while long-term exposure leads to increased ROS generation, decreased mitochondrial activity, and formation of tubular intercellular structures. Macrophages, monocytes, and dendritic cells are affected; exposure results in cytotoxicity, oxidative stress, intracellular Ca 2+ flux, decreased mitochondrial membrane potential, and production of IL-1β and chemokine CXCL9. The NPs are phagocytosed by macrophages and dissolved in lysosomes. In vitro the Comet assay and the cytokinesis-blocked micronucleus assay show genotoxicity, whereas the Ames test does not. This is, however, not confirmed by in vivo genotoxicity assays. Protein binding results in increased stability.

show abstract

Genotoxicity and Cytotoxicity of Zinc Oxide and Titanium Dioxide in HEp-2 Cells

Cited by 143 publications

References 63 publications

Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay

Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay

In vitro and in vivo toxicity assessment of nanoparticles

A review of mammalian toxicity of ZnO nanoparticles

Contact Info

Product

Resources

About