Genotoxicity and oxidative stress biomarkers in Carassius gibelio as endpoints for toxicity testing of Ukrainian polluted river waters

Tsangaris, Catherine; Vergolyas, M. R.; Fountoulaki, Eleni; Гончарук, В. В.

doi:10.1016/j.ecoenv.2011.08.010

Cited by 22 publications

(8 citation statements)

References 39 publications

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“…Currently, fishes of the genus Carassius (Cypriniformes: Cyprinidae), including goldfish, crucian carp and Japanese crucian carp are more and more widely used as test subjects in various ecotoxicological studies (11)(12)(13)(14). These freshwater fish are distributed widely in around the Eurasian continent (15).…”

Section: Introductionmentioning

confidence: 99%

Morphological and hematological parameters of Carassius Gibelio ( Pisces: Gyprinidae ) in conditions of anthropogenic pollution in Southern Bulgaria. Use of hematological parameters as biomarkers

Zhelev¹,

Mollova²,

Boyadziev³

2016

TJS

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Basic morphological and hematological parameters were studied in adult, sexually mature individuals of Carrasius gibelio living in two rivers with varying degrees and different types of anthropogenic pollution (less disrupted site, domestic sewage pollution and heavy metal pollution) in Southern Bulgaria. The study was conducted on 30 female fish, age 3+ from each site. The results show that in C. gibelio, which inhabit the site with heavy metal pollution, the morphological parameters BW and FCF have the lowest statistically significant values. Specimens of C. gibelio from the river site with domestic sewage pollution have the following hematological profiles: erythrocytosis, leukocytosis and hyperchromia, differential leukocyte count: neutrophilia, eosinophilia, monocytosis and lymphocytopenia. Red cell indices (MCH, MCHC and MCV) display the highest statistically significant values. The C. gibelio specimens from the site with heavy metal pollution had the following hematological profiles: erythrocytosis, leukocytopenia and hypоchromia, differential leukocyte count: neutrophilia, eosinophilia, monocytosis and lymphocytopenia. The lowest statistically significant values of red cell indices (MCH, MCHC and MCV) indicate anemic changes of microcytic and hypochromic type. The hematological parameters of C. gibelio are adequate biomarkers of the physiological state of animals and their habitats.

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Section: Introductionmentioning

confidence: 99%

Morphological and hematological parameters of Carassius Gibelio ( Pisces: Gyprinidae ) in conditions of anthropogenic pollution in Southern Bulgaria. Use of hematological parameters as biomarkers

Zhelev¹,

Mollova²,

Boyadziev³

2016

TJS

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“…Malondialdehyde is the final product of lipid peroxidation. Therefore, both the content of malondialdehyde and the activities of antioxidant enzymes can be used to determine the state of oxidative stress in fish (Tsangaris et al, 2011). In this study, the content of MDA in liver increased, indicating oxidized silkworm pupae did induce lipid peroxidation in GIFT.…”

Section: Discussionmentioning

confidence: 99%

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Feng

Wang

Huang

et al. 2021

Aquaculture Research

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Silkworm (Bombyx mori L.) pupae are a high‐quality feed ingredient, rich in protein and fat. However, silkworm pupae are prone to oxidative rancidity due to the high content of unsaturated fatty acids, which limits its large‐scale use in feed. A 56‐day feeding study was conducted to determine the effects of oxidized silkworm pupae on growth performance, structure and function of the intestine, liver and muscle in genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Three approximately isonitrogenous (crude protein: 328 g kg−1) and isolipidic (crude lipid: 56 g kg−1) diets were formulated containing 4% fresh silkworm pupae, lightly oxidized silkworm pupae and highly oxidized silkworm pupae, which named FSP, LOSP and HOSP respectively. Three groups of GIFT with an initial weight of (44.22 ± 0.54) g were randomly assigned to each diet. The results showed that the weight gain rate (WGR), specific growth rate and feed intake decreased with increasing silkworm pupae oxidative levels (p < 0.05). Villus height in LOSP and HOSP significantly decreased with increasing silkworm pupae oxidative levels (p < 0.05), while villus number and muscular thickness of foregut were similar among three groups (p > 0.05). Dietary oxidized silkworm pupae significantly reduced the activity of intestinal Na+–K+–ATPase compared with FSP group (p < 0.05), whereas that was similar in LOSP and HOSP groups (p > 0.05). The activities of intestinal protease, lipase and pepsin in HOSP group were significantly reduced compared with FSP group (p < 0.05), whereas those were similar in LOSP and FSP groups (p > 0.05). Hepatocytes with swelling and nuclear migration occurred in the liver of LOSP and HOSP groups. Malondialdehyde (MDA) content of LOSP and HOSP groups in liver was significantly higher than that in FSP group (p < 0.05). Muscular histological examinations indicated that muscle fibre atrophy occurred in HOSP group. The content of crude protein and crude ash in muscle of HOSP group was significantly reduced compared with FSP and LOSP groups (p < 0.05). ∑n‐3PUFA contents in the muscle of HOSP decreased by 37.28% compared with FSP group (p < 0.05). To sum up, dietary oxidized silkworm pupae inhibited growth, reduced the gastrointestinal digestive and absorptive capacity and hepatic antioxidant capacity, and the negative impacts were increased with the increasing oxidative degree of silkworm pupae. In addition, highly oxidized silkworm pupae have a destructive effect on the histological structure of the foregut, liver and muscle.

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“…In biological investigations, these alterations in antioxidant defences and oxidative damage are used as indicators of oxidative stress (Halliwell and Gutteridge, 2007). To quantify the level of oxidative stress in fish, many studies have employed measures of antioxidant enzymes or oxidative stress indices in terms of thiobarbituric reactive species (TBARS) (Tsangaris et al, 2011a(Tsangaris et al, , 2011b. Because reactive oxygen species are toxic to living beings, animals employ a variety of antioxidant defence systems to keep the redox state of the cell under control.…”

Section: Statement Of Problemmentioning

confidence: 99%

Quantitative Analysis of Nutrients and Biochemical Evaluation in Pomfret Fish (Pampus argenteus) During Frozen Storage

Samantaray¹,

Tripathy²

2021

Int. j. res. appl. sci. biotechnol.

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Marine fish are well-known for being a high-quality protein source having high concentration of essential amino acids. It has high concentration of mono unsaturated and poly unsaturated fatty acids, which may aid in the optimization of lipid profiles and the reduction of the risk of coronary heart disease (CHD). The goal of this study was to estimate the nutritional and biochemical status of raw sea fish Pampus argenteus after 30 days of frozen storage at -200C with 15-day intervals. Nutrient study showed a decrease in protein and lipid content. The changes of hydrogen peroxide and oxidized lipid products were estimated in the muscle tissue during fresh and storage condition. Results indicate that during storage the oxidative stress increased. An antioxidant enzyme (superoxide dismutase, smutase, catalase, glutathione peroxidase) measurement was determined. The increased amount of oxidative stress during fish storage is shown by the differential activity of antioxidant enzymes. The amount of protein in fish varies slightly between species and even within species. Fish is high in protein, vitamins, minerals, and omega-3 fatty acids, which are essential for brain development (Spencer et al., 1971; Jacylin et al., 2010). A well-balanced diet consists variety of fish that can help in children's growth and development as well as their heart health (Jinadasa, 2014).

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Genotoxicity and oxidative stress biomarkers in Carassius gibelio as endpoints for toxicity testing of Ukrainian polluted river waters

Cited by 22 publications

References 39 publications

Morphological and hematological parameters of Carassius Gibelio ( Pisces: Gyprinidae ) in conditions of anthropogenic pollution in Southern Bulgaria. Use of hematological parameters as biomarkers

Morphological and hematological parameters of Carassius Gibelio ( Pisces: Gyprinidae ) in conditions of anthropogenic pollution in Southern Bulgaria. Use of hematological parameters as biomarkers

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Quantitative Analysis of Nutrients and Biochemical Evaluation in Pomfret Fish (Pampus argenteus) During Frozen Storage

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