Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol

Abstract

Tramadol is an analgesic and psychoactive drug that acts primarily upon the central nervous system where it alters brain function, resulting in temporary changes in perception, mood, consciousness and behavior. The aim of present study was to analyze the genotoxicity and repair capability of DNA after Tramadol exposure in albino mice (Mus musculus). For this purpose, forty mice were divided equally into four groups as; a control group (without drug) and three treatment groups that were treated with three doses of Tramadol as minimum dose group, Intermediate dose group and maximum dose group, corresponding to 25 mg/kg, 50 mg/kg and 75 mg/kg of body weight respectively. The dose was given orally for 15 days. After 15 days peripheral blood was drawn from half mice of each group and subjected to comet assay. While the remaining half mice were given a recovery period of 15 days and same procedure was used for blood collection and comet assay. Significant difference in various comet parameters was observed among control and exposed groups. Maximum damage was observed at highest concentration 75 mg/kg of Tramadol and minimum damage was observed at dose 25 mg/kg of Tramadol, while results of repaired mice group showed that repair capability of Tramadol was minor and recovery of Tramadol required a lot of time. It can be concluded that Tramadol cause genotoxicity that is dose dependent and has low repair capability.

Toxicology of tramadol following chronic exposure based on metabolomics of the cerebrum in mice

“…The present study investigated the effect of chronic tramadol administration on the cerebrum of mice based on metabolomics, with a focus on oxidative stress and inflammation as adverse reactions of tramadol. With regard to the dosage of tramadol in animal experiments, we set the dose at 20 mg/kg and 50 mg/kg, which was consistent with a previous study 29 . Compared to the median lethal dose in mice (200-300 mg/kg), the doses used in our animal experiments were lower; however, it is better to study the long-term side effects of tramadol as a painkiller.…”

Mecanismos de genotoxicidade in vivo do tramadol: uma revisão bibliográfica

“…Regarding the consequences that these women face, they vary according to the psychiatric status at the time of the assessment of the crime. [1][2][3] Conclusions: Women with a history of psychiatric illness and with psychological alterations in previous pregnancies are more likely to commit this crime. In addition to these factors, one should be noted that pregnant women who abandon psychotropic medication contribute intensively to infanticide.…”

“…Introdução: O etilenoglicol é um álcool utilizado na produção de anticongelantes, detergentes, tintas e esmaltes, entre outros. A exposição é maioritariamente acidental pelo consumo de bebidas ou alimentos adulterados, porém o sabor adocicado e acessibilidade associada a um baixo custo, promovem o seu consumo como substituinte do etanol [1,2]. Objetivos: Compreender o xenobiótico através da abordagem da sua cinética no organismo, mecanismo de ação, toxicidade e aspetos forenses relacionados com o mesmo.…”

Estudo de biomarcadores de genotoxicidade e nefrotoxicidade decorrentes da exposição a doses terapêuticas de tramadol

“…Resultados: Estudos in vivo demonstraram que o tramadol, em doses terapêuticas, tem potencial genotóxico, sendo a sua genotoxicidade dose-dependente e afetando a capacidade de reparação do DNA. Esta mantém-se reduzida após a descontinuação da administração oral de tramadol [3]. A administração de tramadol induz efeitos neurotóxicos, hepatotóxicos e nefrotóxicos, aumentando os níveis hepáticos e renais de 8-hidroxidesoxiguanosina (8-OHdG) de maneira dose-dependente [5].…”