Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract

Abstract

Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.

Antioxidant effect of Hymenaea courbaril L (Jatob) sap on the healing of wounds on mice

“…Initially, the ethyl acetate extract of the Jatobá sap was evaluated regarding its capacity for sequestering free radicals through the DPPH, ABTS, and SOD tests, presenting a strong antioxidant activity, with effects comparable to the controls used as positive standard for such activities (Table 1). These results corroborate with a previous study in which the same assays were conducted to evaluate the antioxidant potential of the H. courbaril L., but using the hydroethanolic extract from seed, showing its antioxidant potential (Spera et al, 2019).…”

“…Studies carried out with the H. courbaril L species reveal the presence of phenolic compounds, such as tannins, flavonoids, essential oils, terpenes and diterpenes. Regarding their biological action, studies carried out with extracts, fractions or compounds isolated from this plant demonstrate antibacterial activity (Aleixo et al, 2015), anti-inflammatory (Brito et al, 2015), antiviral action (Cecílio et al, 2012), antineoplastic and immunosuppressive activity (Suárez and Chávez, 2018;Spera et al, 2019), action antimicrobial (Correa et al, 2020), muscle relaxant properties (Bezerra et al, 2013) and antifungal and antioxidant activity (Menezes et al, 2020). However, the use of the Jatobá sap for healing skin wounds was not yet studied.…”

>i<Scaffolds>/i< de biopolímeros e resina de jatobá para utilização em engenharia tecidual

“…Pode ser aplicada no tratamento de diversos tecidos, como sistema nervoso central (AMADO et al, 2008;SADEGHI;MOZTARZADEH;MOHANDESI, 2019), para doença de Parkinson (HARRIS et al, 2020), na regeneração dérmica (IACOB et al, 2018;WEISS, 2017), dentária (BELLUCCI et al, 2019), na regeneração óssea e de cartilagem (KAZIMIERCZAK et al, 2019;STUCKENSEN et al, 2019;TOLOUE et al, 2019), pancreática (XU et al, 2019), do fígado (HOSSEINI et al, 2019), vasos sanguíneos e válvula cardíaca (COPES et al, 2019), entre outros. A combinação do uso de scaffolds com a semeadura de células para criar uma estrutura capaz de funcionar como um suporte 3D e que promova a formação de uma nova MEC semelhante ao tecido nativo lesionado é a abordagem mais utilizada na engenharia tecidual (STACE et al, 2015). Nesta técnica as células são coletadas e semeadas em um scaffold poroso biodegradável, com o tamanho e a forma desejados e condicionados em um biorreator in vitro por várias semanas, onde esse scaffold é exposto a sinais bioquímicos e estimulação mecânica que servem para guiar a formação adequada do tipo de tecido a ser substituído (EBRAHIMI; ASIRI; MOHAMMAD, 2018).…”

“…Compostos como os lábdanos presente na resina apresentam atividades antibacteriana, antiinflamatória e antitumoral (GIRÓN et al, 2008), enquanto que os flavonoides mostraram efeito citotóxico para células tumorais, efeito antigenotóxico na medula óssea e atividade antioxidante (SPERA et al, 2019). O ácido copálico (ácido cis/trans labd-8( 20),13-dien-15-oico), por exemplo, demonstrou ser atóxico para células normais além de apresentar atividade antiinflamatória (VARGAS et al, 2015).…”

“…A resina também pode conter flavonoides, lipídios, taninos, procianidinas e óleos essenciais (VALE et al, 2013). Os lábdanos presente na sua composição apresentaram atividades antibacteriana, anti-inflamatória e antitumoral (GIRÓN et al, 2008), já os flavonoides mostraram efeito citotóxico para células tumorais, efeito antigenotóxico na medula óssea e atividade antioxidante (SPERA et al, 2019). O ácido copálico, um ácido resinoso, demonstrou ser atóxico para células normais e ter atividade antiinflamatória (VARGAS et al, 2015).…”