Genotoxicity assessment of root extracts of Paeonia lactiflora Pall

Bak, Su-Min; Back, Seng-Min; Kim, Da Yeon; Jeung, Na-Young; Kim, Nanyoung; Yang, Jun-Young; Han, Kyung-Nam; Kim, Yong-Bum; Min, Byung Sun; Lee, Byoung‐Seok; Park, Seong Hoon

doi:10.1016/j.mrgentox.2022.503579

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…The revertant colony count in the negative control group fell within the range of the historical control data, whereas in all positive control groups, the revertant colony count showed a significant increase (at least two-fold higher) compared to the negative control group. 53 This indicated that the test was performed appropriately and supported the validity of the results.…”

Section: Genotoxicitymentioning

confidence: 62%

“…The Ames test was carried out according to the OECD test guidelines 471, which are based on the method of Maron and Ames with minor modifications. 53 Briefly, S. typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli WP2uvrA (Molecular Toxicology, Inc.) were used as tester strains in the presence or absence of 5% v/v Aroclor 1254-induced rat liver S9 metabolic system (S9 mix). Based on the dose-range test results, 10 and 20 ppm per plate were selected as the maximum concentration for the Ames test of MB (UV + TiO 2 ) and MB (focused ultrasonication).…”

Section: Methodsmentioning

confidence: 99%

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Novel ultrasonic technology for advanced oxidation processes of water treatment

Kim,

Park

et al. 2024

RSC Adv.

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Section: Genotoxicitymentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Novel ultrasonic technology for advanced oxidation processes of water treatment

Kim,

Park

et al. 2024

RSC Adv.

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“…Ames test, which evaluates bacterial reverse mutation, is used worldwide as an initial screening method to determine the mutagenic potential of new chemicals [1][2][3][4][5][6] . In the standard Ames test, Salmonella Typhimurium (TA100, TA98, TA1535, and TA1537) and Escherichia coli (E. coli) (WP2uvrA) strains are used to identify potentially genotoxic substances 7 . Many carcinogens or mutagens remain inactive until they are enzymatically transformed into an electrophilic species that can covalently bind to DNA, causing mutations.…”

Section: Introductionmentioning

confidence: 99%

Differential Genotoxicity of Polygoni Multiflori in Rat and Human: Insights from Ames Test and S9 Metabolic Activation System

Bak,

Back,

Kim

et al. 2024

Preprint

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The Ames test is used worldwide to initially screen the mutagenic potential of new chemicals. In the standard Ames test, S. typhimuriumstrains (TA100, TA98, TA1535, and TA1537) and Escherichia coli (WP2uvrA) are treated with substances with/without cytochrome P450s (CYPs)-induced rat S9 fractions for identifying mutagens and pro mutagens. However, many substances show completely different toxicity patterns depending on whether the liver S9 fraction belongs to rats or humans. The natural product Polygoni Multiflori Radix(PMR)can also show bacterial reverse mutation, followed by the rat or human liver S9 fraction. While PMR elicits reverse mutations in the TA1537 strain in rat liver S9 but not in human liver S9, this mechanism has not been verified yet. To explain this, the differences in metabolic enzymes compositions commonly observed between rats and humans have been implicated. This study aimed to explore the key factors that cause differences in the genotoxicity of PMR between rat and human liver S9 metabolic enzymes. The results of next-generation sequencing (NGS) analysis showed that both rat and human metabolic enzymes caused similar mutations in TA1537. However, when the metabolic enzymes in each S9 fraction were analyzed using ion mobility tandem mass spectrometry (IM-MS), rat- and human-specific enzymes were identified among the cytochrome (CYP) family, especially aryl hydrocarbon receptor (AHR)-related CYPs. These findings suggest that CYP1A1 isoforms contribute to the mechanism of PMR in the Ames test. Therefore, an invitro Ames test might be more reliable in predicting genotoxicity for both rodents and humans. This will also help overcome the limitations of laboratory animal-based toxicity evaluations, which provide unreliable results due to interspecies differences between humans and rodents.

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