Genotoxicity assessment of water soluble fractions of biodiesel and its diesel blends using the Salmonella assay and the in vitro MicroFlow® kit (Litron) assay

Leme, Daniela Morais; Grummt, Tamara; Oliveira, Danielle Palma de; Sehr, Andrea; Renz, Sylvia; Reinel, Sissy; Ferraz, Elisa Raquel Anastácio; Marchi, Mary Rosa Rodrigues de; Machado, Marcos Canto; Zocolo, Guilherme Julião; Marin-Morales, Maria Aparecida

doi:10.1016/j.chemosphere.2011.10.017

Cited by 28 publications

(13 citation statements)

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“…Some toxic effects of biodiesel and biodiesel blends with petroleum diesel have been reported for different organisms. In accordance to this, it has been demonstrated that biodiesel promotes mutagenic and genotoxic effects in Salmonella typhimurium (Leme et al, 2012) and oxidative stress in freshwater fish (Nogueira et al, 2011). The toxic mechanisms promoted by biodiesel is not fully understood, but is probably related to the presence of additives and impurities in the commercial formulations.…”

Section: Introductionmentioning

confidence: 85%

Biochemical responses in mussels Perna perna exposed to diesel B5

et al. 2015

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Section: Introductionmentioning

confidence: 85%

Biochemical responses in mussels Perna perna exposed to diesel B5

et al. 2015

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“…Finally, the samples were dried under a gentle nitrogen stream. For the Ames test, these extracts were dissolved in DMSO2526 and were matched into a specific concentration series to make 0.1 mL solutions containing organic compounds from 0.5 L, 1 L, and 2 L water to produce 0.5, 1.0, and 2.0 L equivalent/plate of water samples, respectively. The concentrates were stored at −20°C for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Mutagenicity of drinking water sampled from the Yangtze River and Hanshui River (Wuhan section) and correlations with water quality parameters

Yang

et al. 2015

Sci Rep

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A total of 54 water samples were collected during three different hydrologic periods (level period, wet period, and dry period) from Plant A and Plant B (a source for Yangtze River and Hanshui River water, respectively), and several water parameters, such as chemical oxygen demand (COD), turbidity, and total organic carbon (TOC), were simultaneously analyzed. The mutagenicity of the water samples was evaluated using the Ames test with Salmonella typhimurium strains TA98 and TA100. According to the results, the organic compounds in the water were largely frame-shift mutagens, as positive results were found for most of the tests using TA98. All of the finished water samples exhibited stronger mutagenicity than the relative raw and distribution water samples, with water samples collected from Plant B presenting stronger mutagenic strength than those from Plant A. The finished water samples from Plant A displayed a seasonal-dependent variation. Water parameters including COD (r = 0.599, P = 0.009), TOC (r = 0.681, P = 0.02), UV254 (r = 0.711, P = 0.001), and total nitrogen (r = 0.570, P = 0.014) exhibited good correlations with mutagenicity (TA98), at 2.0 L/plate, which bolsters the argument of the importance of using mutagenicity as a new parameter to assess the quality of drinking water.

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“…This model evaluates the dose-response effect by calculating the analysis of variance (AN-OVA) between the number of revertants at different concentrations and the negative control, followed by linear regression (Leme et al, 2012). The results were considered positive when there was a positive dose-response and a significant statistical difference between the tested concentrations and the negative control.…”

Section: Ames Test (Salmonella/microsome Preincubation Assay)mentioning

confidence: 99%