Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Yin, Dandan; Xu, Zhenbiao; Feng, Minmin; Zhao, Zelong; Chen, Dahu; Lin-xia, Song

doi:10.1155/2022/2822605

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Some studies have suggested that metformin's antioxidant effects may help protect against DNA damage caused by oxidative stress in fish. Our findings are supported by the fact that planarians exposed to 10 mmol/L of metformin for 1, 3, and 5 days exhibited 10.2%, 25.4%, and 36.8% increases in DNA damage, whereas planarians exposed to 50 mmol/L of metformin experienced increases of 40.6%, 62.8%, and 65.4% in DNA damage [31]. The research on the effects of metformin on DNA damage in fish is still limited, and more studies are needed to fully understand the potential risks.…”

Section: Genotoxicitysupporting

confidence: 81%

“…Today, there are more genotoxic pollutants than ever in the aquatic environment, spurring researchers to create rapid monitoring tools. Due to their accessibility, speed, and sensitivity, comet test methodologies are frequently used in ecotoxicology and biomonitoring [31]. Direct DNA damage may occur when environmental contaminants, such as pharmaceutical medications, combine with aquatic creatures; indirect DNA damage can occur when these toxins generate reactive oxygen species (ROS), which have the potential to disrupt the consistency of DNA pieces.…”

Section: Genotoxicitymentioning

confidence: 99%

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Biochemical Patterns and Genotoxicity of the Endocrine Disruptor Metformin in the Freshwater Fish Labeo rohita

Sibiya

Al‐Ghanim

Govindarajan

et al. 2023

Fishes

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Metformin is one of the most extensively used drugs, making it one of the most likely endocrine disruptors in the environment, which may negatively affect fish and other freshwater animals. Still, there is a dearth of studies examining metformin’s impact on freshwater creatures, like fish. This research aimed to identify the biochemical and genotoxicity effects of the endocrine disruptor metformin in the freshwater fish Labeo rohita at ecologically appropriate doses. Metformin’s toxicity was evaluated by subjecting L. rohita to the drug over 28 days at two dosages (40 µg/L to 80 µg/L). The results indicated that 40 µg/L and 80 µg/L of metformin caused an increase in reactive oxygen species and the generation of free radicals in the body of L. rohita, which in turn caused impairment and alterations in total hemoglobin, red blood corpuscles, white blood corpuscles, oxidative stress, lipid peroxidation, protein carbonyl activity, respiratory burst activity, myeloperoxidase activity, and lysozyme activity. In addition, animals treated with the maximum metformin dose (80 µg/L) demonstrated substantial DNA damage in the genotoxicity experiment. Metformin’s endocrine-disrupting actions may have unintended ramifications for the well-being of aquatic species in their natural habitats. Results of the study demonstrated a serious concern that metformin exposure might be harmful to aquatic life.

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Section: Genotoxicitysupporting

confidence: 81%

Section: Genotoxicitymentioning

confidence: 99%

Biochemical Patterns and Genotoxicity of the Endocrine Disruptor Metformin in the Freshwater Fish Labeo rohita

Sibiya

Al‐Ghanim

Govindarajan

et al. 2023

Fishes

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“…Randomly amplified polymorphic DNA (RAPD) is a technique for polymorphism analysis of genomic DNA, which is often used for the detection of genetic diversity and genotoxicity analysis ( Pandey, Nagpure & Trivedi, 2018 ; Zare et al, 2019 ; Zhou et al, 2011 ). It is an important method for detecting the genotoxicity of drugs and pollutants to planarians ( Yin et al, 2022 ; Zhang et al, 2016b ). However, the application of RAPD technology for detecting the genotoxicity of SDS to planarians has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Toxic effects of sodium dodecyl sulfate on planarian Dugesia japonica

Feng

Yin

et al. 2023

PeerJ

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Sodium dodecyl sulfate (SDS) is an anionic surfactant, which is widely used in various fields in human life. However, SDS discharged into the water environment has a certain impact on aquatic organisms. In this study, planarian Dugesia japonica (D. japonica) was used to identify the toxic effects of SDS. A series of SDS solutions with different concentrations were used to treat planarians for the acute toxicity test , and the results showed that the semi-lethal concentration (LC50) of SDS to D. japonica at 24 h, 48 h, 72 h, and 96 h were 4.29 mg/L, 3.76 mg/L, 3.45 mg/L, and 3.20 mg/L respectively. After the planarians were exposed to 0.5 mg/L and 1.0 mg/L SDS solutions for 1, 3, and 5 days, the activities of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) content were measured to detect the oxidative stress and lipid peroxidation in planarians. Random amplified polymorphic DNA (RAPD) analysis was performed to detect the genotoxicity caused by SDS to planarians. The results showed that the activities of SOD, CAT, and MDA content increased after the treatment, indicating that SDS induced oxidative stress in planarians. RAPD analysis showed that the genomic template stability (GTS) values of planarians treated by 0.5 mg/L and 1.0 mg/L SDS for 1, 3, and 5 days were 67.86%, 64.29%, 58.93%, and 64.29%, 60.71%, 48.21%, respectively. GTS values decreased with the increasing of SDS concentration and exposure time, indicating that SDS had genotoxicity to planarians in a time and dose-related manner. Fluorescent quantitative PCR (qPCR) was used to investigate the effects of SDS on gene expression of planarians. After the planarians were exposed to 1.0 mg/L SDS solution for 1, 3, and 5 days, the expression of caspase3 was upregulated, and that of piwiA, piwiB, PCNA, cyclinB, and RAD51 were downregulated. These results suggested that SDS might induce apoptosis, affect cell proliferation, differentiation, and DNA repair ability of planarian cells and cause toxic effects on planarian D. japonica.

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Embryotoxicity Produced by the Mixture of Aluminum, Metformin and Penicillin on Common Carp (Cyprinus carpio): a Study of Interactions

Cano-Viveros

Galár-Martínez²,

García-Medina³

et al. 2022

Water Air Soil Pollut

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Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Cited by 3 publications

References 31 publications

Biochemical Patterns and Genotoxicity of the Endocrine Disruptor Metformin in the Freshwater Fish Labeo rohita

Biochemical Patterns and Genotoxicity of the Endocrine Disruptor Metformin in the Freshwater Fish Labeo rohita

Toxic effects of sodium dodecyl sulfate on planarian Dugesia japonica

Embryotoxicity Produced by the Mixture of Aluminum, Metformin and Penicillin on Common Carp (Cyprinus carpio): a Study of Interactions

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