Genotoxicity in the freshwater gastropod<i>Lymnaea luteola</i>L: assessment of cell type sensitivities to lead nitrate

Ali, Daoud; Ali, Huma; Alarifi, Saud; Alamer, Ali; Kumar, Phool Gend; Kumar, Sudhir; Masih, Agha Parvez; Alkahtani, Saad; Alkahtane, Abdullah A.; Hussain, Syed

doi:10.1080/02757540.2016.1275587

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…Copper sulfate has been reported to cause deleterious effects at higher levels ( 53 – 55 ). Sometimes the water quality, like pH, dissolved organic carbon, and hardness, also affect the toxicity of metal (e.g., Cu, Zn, Ni) in aquatic environments ( 22 ). Among all the environmental issues, metal pollution is the most serious and major hazard to the safety of humans and worldwide ecosystems ( 21 , 44 , 56 – 58 ).…”

Section: Discussionmentioning

confidence: 99%

Copper sulfate induces clinico-hematological, oxidative stress, serum biochemical and histopathological changes in freshwater fish rohu (Labeo rohita)

Naz

Hussain²,

Guangbin³

et al. 2023

Front. Vet. Sci.

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Despite being an essential trace element for numerous metabolic processes and micronutrients, copper (Cu) has induced adverse effects on the environment and public health due to its continuous and widespread use for the last several decades. The current study assessed the hematological and histopathological alterations in the freshwater fish (Labeo rohita) exposed to graded concentrations of copper sulfate. For this purpose, L. rohita fish (n = 72), weighing ~200–215 g, were randomly divided into four experimental groups and then exposed to acute doses of CuSO4, i.e., control, 0.28, 0.42, and 0.56 μgL−1. For comparative analysis of hematological and biochemical changes, blood/serum samples were obtained on 12, 24, and 36 days. Overall, the body weight of fish decreased with the time and dose of CuSO4; as the dose increases, body weight decreases. Dose and time-dependent results were observed in other parameters also. Results showed a significant increase in leukocytes, whereas red blood cells count, Hb, and Hct were significantly reduced in treated groups compared to the control. The mean corpuscular hemoglobin (MHC) and mean corpuscular hemoglobin concentration (MCHC) showed a non-significant decrease in treated groups compared to the control group. Serum biochemical parameters, including total proteins, albumin, and globulin, decreased significantly (p < 0.05). At the same time, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glucose, and cholesterol were significantly (p < 0.05) increased in the treated groups compared to the control group. Significantly (p < 0.05) increased levels of lipid peroxidation while decreased values of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (RGSH) in the blood of fish were recorded. Histopathological examination of fish gills, liver, and kidneys showed inflammation and degenerative changes due to CuSO4 exposure. In the brain tissue, degenerative changes like neuron necrosis, intracellular edema, cytoplasmic vacuolization, and congestion were observed. In conclusion, the study indicates that exposure to copper sulfate, even in smaller concentrations, can cause adverse hematological and histopathological changes in L. rohita fish.

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Section: Discussionmentioning

confidence: 99%

Copper sulfate induces clinico-hematological, oxidative stress, serum biochemical and histopathological changes in freshwater fish rohu (Labeo rohita)

Naz

Hussain²,

Guangbin³

et al. 2023

Front. Vet. Sci.

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“…[42][43][44][45] Excess ROS will damage the antioxidant capacity, cause mitochondria injury and genotoxicity and lead to cell apoptosis. 46,47 Mitochondria are the primary source of endogenous ROS, mitochondria dysfunction will lead to increased oxidative stress, which results in the production of lipid peroxidation, and MDA is one of the indicators of oxidative damage. 46,48 In this study, BPAF treatment induced mitochondrial function damage, suggesting that BPAF may cause the production of ROS in cells.…”

Section: Discussionmentioning

confidence: 99%

Curcumin alleviates bisphenol AF‐induced oxidative stress and apoptosis in caprine endometrial epithelial cells via the Nrf2 signaling pathway

Liu

Wang

et al. 2023

Environmental Toxicology

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Bisphenol AF (BPAF), a BPA‐substitute, has been widely used in industrial compounds throughout the world. Several studies have shown that BPAF has endocrine interference and reproductive toxicity. However, the toxic effects of BPAF on pregnancy and placenta of goats are still unclear. Therefore, the objective of this study was to reveal the toxic effect of BPAF by using an in vitro culture model of caprine endometrial epithelial cells (EECs) and further attempted to alleviate the toxicity by curcumin pretreatment. The results showed that BPAF induces significant effects on EECs, including decreased cell viability and mitochondrial membrane potential (△ψm), elevating intracellular reactive oxygen species (ROS), promoting cell apoptosis through upregulating the expression of Bax, Cytochrome c, and downregulating the expression of Bcl‐2. Meanwhile, BPAF induced dysregulation of oxidative stress by increasing the levels of malondialdehyde (MDA) and glutathione peroxidase (GSH‐Px) but decreasing the activities of superoxide dismutase (SOD). However, curcumin pretreatment could significantly attenuate BPAF‐induced toxic effects in EECs. Further study revealed that BPAF treatment could activate mitogen‐activated protein kinase (MAPK) pathway and nuclear factor‐erythroid 2‐related factor 2 (Nrf2) expression, but curcumin pretreatment significantly inhibited the activation of MAPK signal pathway and Nrf2 expression induced by BPAF. Overall, this study indicated that curcumin could prevent BPAF‐induced EECs cytotoxicity, which provides a potential therapeutic strategy for female infertility associated with BPAF exposure.

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“…The studies were done on several cell types from embryonic cells to adult cells such as haemocytes, gill cells, hepatopancreas cells and digestive gland cells. The majority of studies are on freshwater snails employing different species including Lymnaea stagnalis [158][159][160][161], Lymnaea luteola [162][163][164][165], Biomphalaria glabrata [166], Biomphalaria alexandrina [167,168], Marisa cornuarietis [169], Potamopyrgus antipodarum [170], Bellamya aeruginosa [171], Pila globose [172], Viviparous bengalensis [149] and Heleobia cf. australis [173].…”

Section: Gastropodsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Genotoxicity in the freshwater gastropodLymnaea luteolaL: assessment of cell type sensitivities to lead nitrate

Cited by 12 publications

References 21 publications

Copper sulfate induces clinico-hematological, oxidative stress, serum biochemical and histopathological changes in freshwater fish rohu (Labeo rohita)

Copper sulfate induces clinico-hematological, oxidative stress, serum biochemical and histopathological changes in freshwater fish rohu (Labeo rohita)

Curcumin alleviates bisphenol AF‐induced oxidative stress and apoptosis in caprine endometrial epithelial cells via the Nrf2 signaling pathway

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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