Genotoxicity of complex PAH mixtures recovered from contaminated lake sediments as assessed by three different methods

Randerath, Kurt; Randerath, Erika; Zhou, Guo Dong; Supunpong, N.; He, L.Y.; McDonald, Thomas J.; Donnelly, Kirby C.

doi:10.1002/(sici)1098-2280(1999)33:4<303::aid-em7>3.0.co;2-0

Cited by 24 publications

(17 citation statements)

References 39 publications

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“…The finding of increases into toxicity are not without precedent. Thus, a rise in genotoxicity has also been reported in solvent extracts of soils amended with diesel fuel and subjected to biodegradation in the laboratory (Randerath et al, 1999). Acutely toxic products have also been reported to be formed during the course of biodegradation (Wang and Bartha, 1994).…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity is Unrelated to Total Concentration of Priority Carcinogenic Polycyclic Aromatic Hydrocarbons in Soils Undergoing Biological Treatment

2002

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A solid-phase microbiological assay was used to determine the changes in genotoxicity associated with sequestration or biodegradation of carcinogenic compounds in contaminated soils. The concentration of six carcinogenic polycyclic aromatic hydrocarbons (PAHs) did not change in 59 d in sterile soil, but the genotoxicity declined markedly. In a soil undergoing bioremediation in the field for 147 d or biodegradation in the laboratory for 180 d, the concentrations either changed little or declined at different rates, but the genotoxicity increased followed by a decline. The genotoxicity of a second soil declined as a result of biological treatment. The data show that genotoxicity of contaminated soils may be unrelated to the concentration of carcinogenic PAHs because of aging or new mutagens formed during biological treatment.

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Section: Discussionmentioning

confidence: 99%

Genotoxicity is Unrelated to Total Concentration of Priority Carcinogenic Polycyclic Aromatic Hydrocarbons in Soils Undergoing Biological Treatment

2002

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“…Indeno(1,2,3-c,d)pyrene (IP) was purchased from Absolute Standards (Hamden, CT). Materials for DNA extraction [24], [25], [26], [27], RNA extraction [28], and 32 postlabeling analysis [24], [29], [30] have been reported previously.…”

Section: Methodsmentioning

confidence: 99%

Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse

Zhou

Zhu²,

Phillips

et al. 2011

PLoS ONE

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Many carcinogenic polycyclic aromatic hydrocarbons (PAHs) and their metabolites can bind covalently to DNA. Carcinogen-DNA adducts may lead to mutations in critical genes, eventually leading to cancer. In this study we report that fish oil (FO) blocks the formation of DNA adducts by detoxification of PAHs. B6C3F1 male mice were fed a FO or corn oil (CO) diet for 30 days. The animals were then treated with seven carcinogenic PAHs including benzo(a)pyrene (BaP) with one of two doses via a single intraperitoneal injection. Animals were terminated at 1, 3, or 7 d after treatment. The levels of DNA adducts were analyzed by the 32P-postlabeling assay. Our results showed that the levels of total hepatic DNA adducts were significantly decreased in FO groups compared to CO groups with an exception of low PAH dose at 3 d (P = 0.067). Total adduct levels in the high dose PAH groups were 41.36±6.48 (Mean±SEM) and 78.72±8.03 in 109 nucleotides (P = 0.011), respectively, for the FO and CO groups at 7 d. Animals treated with the low dose (2.5 fold lower) PAHs displayed similar trends. Total adduct levels were 12.21±2.33 in the FO group and 24.07±1.99 in the CO group, P = 0.008. BPDE-dG adduct values at 7 d after treatment of high dose PAHs were 32.34±1.94 (CO group) and 21.82±3.37 (FO group) in 109 nucleotides with P value being 0.035. Low dose groups showed similar trends for BPDE-dG adduct in the two diet groups. FO significantly enhanced gene expression of Cyp1a1 in both the high and low dose PAH groups. Gstt1 at low dose of PAHs showed high levels in FO compared to CO groups with P values being 0.014. Histological observations indicated that FO played a hepatoprotective role during the early stages. Our results suggest that FO has a potential to be developed as a cancer chemopreventive agent.

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“…Indeno(1,2,3-c,d)pyrene (IP) was purchased from Absolute Standards (Hamden, CT). Materials for DNA extraction (Gupta 1984; Randerath et al 1981; Reddy and Randerath 1986; Zhou et al 1999), and 32 postlabeling analysis (Phillips and Arlt 2007; Randerath et al 1999; Reddy and Randerath 1986; Zhou et al 2004) have been reported previously.…”

Section: Methodsmentioning

confidence: 99%

Mechanistic relationships between hepatic genotoxicity and carcinogenicity in male B6C3F1 mice treated with polycyclic aromatic hydrocarbon mixtures

et al. 2014

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The genotoxicity of a complex mixture [neutral fraction (NF)] from a wood preserving waste and reconstituted mixture (RM) mimicking the NF with seven major polycyclic aromatic hydrocarbons (PAHs) and benzo(a)pyrene (BaP) was investigated by determining DNA adducts and tumor incidence in male B6C3F1 mice exposed to 3 different doses of the chemical mixtures. The peak values of DNA adducts were observed after 24 h and the highest levels of PAH-DNA adducts were exhibited in mice administered NF+BaP, and the highest tumor incidence and mortality were also observed in this group. DNA adduct levels after 1, 7, or 21 d were significantly correlated with animal mortality and incidence of total tumors including liver, lung, and forestomach. However, only hepatic DNA adducts after 7 d significantly correlated with liver tumor incidence. Most proteins involved in DNA repair including ATM, pATR, Chk1, pChk1, DNA PKcs, XRCC1, FANCD2, Ku80, Mre11 and Brca2 were significantly lower in liver tumor tissue compared to non-tumor tissue. Expression of proteins involved in apoptosis and cell cycle regulation were also significantly different in tumor vs non-tumor tissues and it is possible that PAH-induced changes in these gene products are important for tumor development and growth.

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Genotoxicity of complex PAH mixtures recovered from contaminated lake sediments as assessed by three different methods

Cited by 24 publications

References 39 publications

Genotoxicity is Unrelated to Total Concentration of Priority Carcinogenic Polycyclic Aromatic Hydrocarbons in Soils Undergoing Biological Treatment

Genotoxicity is Unrelated to Total Concentration of Priority Carcinogenic Polycyclic Aromatic Hydrocarbons in Soils Undergoing Biological Treatment

Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse

Mechanistic relationships between hepatic genotoxicity and carcinogenicity in male B6C3F1 mice treated with polycyclic aromatic hydrocarbon mixtures

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