Genotoxicity of industrial effluents, river waters, and their fractions using the Ames test and in vitro cytogenetic assay

Černá, Milena; Pastorková, Anna; Šmı́d, Jiři; Bavorová, H; Očadlíková, Dana; Rössner, Pavel; Zavadil, J.

doi:10.1016/0378-4274(96)03737-x

Cited by 19 publications

(5 citation statements)

References 15 publications

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“…Many of these pollutants have been identified as genotoxic, and investigations with the umu test revealed a mutagenic potential of more heavily contaminated old Neckar sediments [12]. Because such old sediments may be remobilized during floods [3], mutagenicity was determined in parallel to cytotoxicity by means of the Ames test [13], for which an enormous body of reference data for pure substances [14], native surface waters [15,16], wastewaters [17,18], and SPM [19] are available.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Settling Particulate Matter and Sediments of the Neckar River (Germany) During a Winter Flood

Hollert¹,

Dürr²,

Erdinger³

et al. 2000

Environ Toxicol Chem

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To investigate the cytotoxic and genotoxic potentials of settling particulate matter (SPM) carried by the Neckar River, a well-studied model for a lock-regulated river in central Europe, during a flood, acute cytotoxicity was investigated using the fibroblast-like fish cell line RTG-2 with the neutral red retention, the succinic acid dehydrogenase (MTT), and the lactatedehydrogenase (LDH) release assays as well as microscopic inspection as endpoints. Genotoxicity of water, pore water, sediments, and SPM were assessed using the Ames test. Different extraction methods (Soxhlet extraction with solvents of variable polarity as well as a fluid/fluid extraction according to pH) in addition to a supplementation of biotests with S9 fractions from the liver of ␤naphthoflavone/phenobarbital-induced rats allowed a further characterization of the biological damage. Both sediments and SPM extracts caused cytotoxic effects in RTG-2 cells. Cytotoxicity was found to increase significantly with polarity of extracting solvents (NR50 ϭ effective concentration for 50% cell death in the neutral red test: 80 [65], 100 [70], 180 [220], and 225 [270] mg/ml for ethanol, acetone, dichloromethane, and n-hexane extracts, respectively, if measured with [without] S9 supplementation). Following extraction according to pH, cytotoxicity could be attributed mainly to neutral substances (NR50: 80 and 218 mg dry SPM/ml test medium for the neutral and the acid fractions, respectively), whereas the slightly acid and basic fractions already showed little or no cytotoxicity. Samples taken during the period of flood rise showed the highest cytotoxic activities. Cytotoxicity was significantly enhanced by the addition of S9 preparations. In contrast, no genotoxic activity was found in native surface waters, pore waters, and SPM.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Settling Particulate Matter and Sediments of the Neckar River (Germany) During a Winter Flood

Hollert¹,

Dürr²,

Erdinger³

et al. 2000

Environ Toxicol Chem

View full text Add to dashboard Cite

show abstract

“…Many of these pollutants have been identified as genotoxic, and investigations with the umu test revealed a mutagenic potential of more heavily contaminated old Neckar sediments [12]. Because such old sediments may be remobilized during floods [3], mutagenicity was determined in parallel to cytotoxicity by means of the Ames test [13], for which an enormous body of reference data for pure substances [14], native surface waters [15, 16], wastewaters [17, 18], and SPM [19] are available.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of settling particulate matter and sediments of the Neckar River (Germany) during a winter flood

Hollert

Dürr

Erdinger

et al. 2000

Enviro Toxic and Chemistry

119

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Abstract-To investigate the cytotoxic and genotoxic potentials of settling particulate matter (SPM) carried by the Neckar River, a well-studied model for a lock-regulated river in central Europe, during a flood, acute cytotoxicity was investigated using the fibroblast-like fish cell line RTG-2 with the neutral red retention, the succinic acid dehydrogenase (MTT), and the lactatedehydrogenase (LDH) release assays as well as microscopic inspection as endpoints. Genotoxicity of water, pore water, sediments, and SPM were assessed using the Ames test. Different extraction methods (Soxhlet extraction with solvents of variable polarity as well as a fluid/fluid extraction according to pH) in addition to a supplementation of biotests with S9 fractions from the liver of ␤-naphthoflavone/phenobarbital-induced rats allowed a further characterization of the biological damage. Both sediments and SPM extracts caused cytotoxic effects in RTG-2 cells. Cytotoxicity was found to increase significantly with polarity of extracting solvents (NR50 ϭ effective concentration for 50% cell death in the neutral red test: 80 [65], 100 [70], 180 [220], and 225 [270] mg/ml for ethanol, acetone, dichloromethane, and n-hexane extracts, respectively, if measured with [without] S9 supplementation). Following extraction according to pH, cytotoxicity could be attributed mainly to neutral substances (NR50: 80 and 218 mg dry SPM/ml test medium for the neutral and the acid fractions, respectively), whereas the slightly acid and basic fractions already showed little or no cytotoxicity. Samples taken during the period of flood rise showed the highest cytotoxic activities. Cytotoxicity was significantly enhanced by the addition of S9 preparations. In contrast, no genotoxic activity was found in native surface waters, pore waters, and SPM.

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“…The Ames plate incorporation method [ [70] , [71] , [72] ] and SOS Chromotest [ 73 , 74 ] have been widely employed in evaluating the genotoxicity of various industrial waste streams and waste leachates. Conversely, the high-throughput Ames MPF method is less commonly utilized in industrial toxicity assessments [ 75 ].…”

Section: Resultsmentioning

confidence: 99%

Industrial ecotoxicology in focus: The unexplored environmental impacts of pilot-scale advanced filtration in Sc recovery

Fekete-Kertész,

Márton,

Molnár

et al. 2024

Heliyon

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Genotoxicity of industrial effluents, river waters, and their fractions using the Ames test and in vitro cytogenetic assay

Cited by 19 publications

References 15 publications

Cytotoxicity of Settling Particulate Matter and Sediments of the Neckar River (Germany) During a Winter Flood

Cytotoxicity of Settling Particulate Matter and Sediments of the Neckar River (Germany) During a Winter Flood

Cytotoxicity of settling particulate matter and sediments of the Neckar River (Germany) during a winter flood

Industrial ecotoxicology in focus: The unexplored environmental impacts of pilot-scale advanced filtration in Sc recovery

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