Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems

Totsuka, Yukari; Higuchi, Takashi; Imai, Toshio; Nishikawa, Akiyoshi; Nohmi, Takehiko; Kato, Tatsuya; Masuda, Shuich; Kinae, Naohide; Hiyoshi, Kyoko; Ogo, Sayaka; Kawanishi, Michihiro; Yagi, Takashi; Ichinose, Takamichi; Fukumori, Nobutaka; Watanabe, Masatoshi; Sügimura, Takashi; Wakabayashi, Keiji

doi:10.1186/1743-8977-6-23

Cited by 90 publications

(68 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the comet assay mean found in the group non-exposed to asbestos was 11 ± 2 AU. This is within the range found in the literature in several populations, reinforcing the fact that there was a statistically significant difference in DNA damage levels between the two groups, with the implication that these results were caused by exposure to asbestos [18,[28][29][30].…”

Section: Group Exposed To Asbestossupporting

confidence: 87%

Evaluation of Genotoxic Effects of Asbestos on Occupationally Exposed Workers in Brazil

Amaral¹,

Joca²,

Carvalho³

et al. 2016

Biomonitoring

View full text Add to dashboard Cite

group and individuals without asbestosis, respectively. Linear regression analysis showed that 28,4% and 50,5% of comet assay results were increased by exposure to asbestos and developed asbestosis. The results of CAT and GST were not difference between the groups. These results supports the association of genotoxic damage and the onset of asbestosis by chrysotile asbestos exposure in workers of this study.Keywords: asbestos, occupational exposure, genotoxicity. IntroductionAsbestosis is a form of pneumoconiosis caused by inhaling asbestos, a generic commercial designation for a group of naturally-occurring mineral silicate fibres of the serpentine (chrysotile asbestos) and amphibole series (actinolite, amosite, anthophyllite, crocidolite and tremolite) [1]. Inhaling any kind of asbestos fibers causes the development of intense interstitial pulmonary fibrosis, which involves an inflammatory reaction, production of collagen and the formation of granuloma [2][3][4][5].Asbestosis and other diseases related to asbestos are increasing worldwide [6,7] and several studies have shown that exposure to asbestos has contributed to occupationally-related cancer. Each year, approximately 125 million people around the world are occupationally exposed to asbestos and at least 100,000 people die because of diseases related to this exposure (for example asbestos-related lung cancer, mesothelioma, or asbestosis [1]) despite the fact that "chrysotile manufacturers, governments of asbestos-producing nations, and some asbestos miners' unions contend either that their products do not cause disease or that there is insufficient evidence to reach a reliable conclusion", as stated by Pezerat (2009) [8]. Abstract: Genotoxic effects of occupational workers exposed to asbestos can be evaluated using different biomarkers as oxidative stress enzymes in conjuction with comet assay. This study assessed changes to oxidative stress enzymatic parameters and genotoxic damage in workers occupationally exposed and non-exposed to chrysotile asbestos, who attended the outpatient Clinic of the Center for Worker Health Studies and Human Ecology (CESTEH/ENSP/FIOCRUZ) in Brazil. Chest radiography and spirometry were performed to assess clinical progression of symptoms. The traditional visual score comet assay in peripheral whole blood cells was used to assess DNA damage, and oxidative stress was evaluated by measuring catalase (CAT) and glutathione S-transferase (GST) activities. Respiratory alterations were observed in 53% of workers exposed, as determined by pulmonary function and bronchodilation, and 6 workers were diagnosed with asbestosis. The comet assay was statistically significantly higher in the exposed group and individuals with asbestosis compared to the non-exposed

show abstract

Section: Group Exposed To Asbestossupporting

confidence: 87%

Evaluation of Genotoxic Effects of Asbestos on Occupationally Exposed Workers in Brazil

Amaral¹,

Joca²,

Carvalho³

et al. 2016

Biomonitoring

View full text Add to dashboard Cite

show abstract

“…In both studies, however, the bone marrow was not exposed to C60 directly. A recent report showed that intratracheal instillation of C60 increased both mutation frequency (gpt assay) and DNA damage (comet assay) in the lung (3). From the mutation spectra, it was suggested that oxidative DNA damage might be involved in mutagenicity of C60 (3).…”

Section: Discussionmentioning

confidence: 99%

“…Reports on the in vivo genotoxicity of C60, however, are con‰icting. It was reported that intratracheal instillation of C60 increased both mutation frequency detected by gpt-assay and DNA damage detected by comet assay in lung (3). Nevertheless another group showed that treatment with C60 by gavage has no genotoxical eŠect in ICR mice, using in vivo micronucleus test in bone marrow cells (4).…”

Section: Introductionmentioning

confidence: 99%

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Horibata

Ukai

Naoki

et al. 2011

Genes and Environment

View full text Add to dashboard Cite

Carbon nanoparticles, such as carbon nanotubes and fullerene (C 60 ), are potential candidates as leading substances in nanotechnologicalˆelds, but little is known about their safety. Here we examined in vivo genotoxicity of C 60 , by performing the Pig-A gene mutation assay in the peripheral blood of male C57BL/6Cr mice. Mice were given single intraperitoneal injection of 3 mg of C 60 particles in 0.5 mL suspension containing 0.1%-Tween80-saline. As a positive control for the Pig-A gene mutation assay, mice were given a single oral administration of N-nitroso-Nethylurea. At 2 and 8 weeks after treatments, we analyzed CD24-negative and -positive red blood cells in peripheral blood and calculated Pig-A mutant frequencies. As a result, we detected no signiˆcant diŠerences in the mutant frequencies between C 60 treated and non-treated mice, indicating that C 60 is negative for genotoxicity in vivo in the limited target tissues assessed in this study. For the full assessment, we need comprehensive whole body survey on the genotoxicity of C 60 .

show abstract

“…The MN test was carried out as described previously (Totsuka et al, 2009). Briefly, human lung carcinoma A549 cells and Chinese hamster ovary (CHO) AA8 cells obtained from the RIKEN Cell Bank (Wako, Japan) were cultured in Eagle's minimum essential medium (Nissui Pharmaceutical Co. Ltd., Tokyo, Japan) supplemented with 10% fetal bovine serum (JRH Biosciences, Lenexa, KS, USA) at 37ºC in a 5% CO 2 atmosphere.…”

Section: Micronucleus Testmentioning

confidence: 99%

Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

-We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours' treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 μg/ml (34 μg/cm 2 ), respectively. In A549 cells, treatment with the nanoparticles (2 μg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 μg/ml (0.34 μg/cm 2 ) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nanoparticles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

show abstract

Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems

Cited by 90 publications

References 51 publications

Evaluation of Genotoxic Effects of Asbestos on Occupationally Exposed Workers in Brazil

Evaluation of Genotoxic Effects of Asbestos on Occupationally Exposed Workers in Brazil

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells

Contact Info

Product

Resources

About