Genotoxicity of Single-Walled Carbon Nanotubes: In Vitro Study on Human Embryonic Fibroblast Cells

Abstract

The effects of single-walled carbon nanotubes on the levels of DNA aberrations, chromosome and genome disorders were studied on human embryonic fibroblasts, their karyotype was analyzed by the spectral karyotyping method. The level of DNA aberrations increased after 3-h exposure to the nanotubes. No appreciable increase in the incidence of aberrant metaphases, micronuclei, and chromosome 1, 6, 8, 11, X, and Y aneuploidy after 24- and 48-h incubation with the nanotubes were detected.

Synthesis and characterization of single‐walled carbon nanotube: Cyto‐genotoxicity in Allium cepa root tips and molecular docking studies

“…CAs were also induced by SWCNTs exposure in immortalized human bronchial epithelial cells and primary human respiratory epithelial cells due to disruption of the mitotic spindle by SWCNTs (Sargent et al 2009, 2012), on Swiss–Webster mice through activation of oxidative stress by functionalized SWCNTs (Patlolla et al, 2016), on the mouse macrophage cell line due to enhanced of ROS production (Di Giorgio et al, 2011) and on human lymphocytes (Catalán et al, 2012). Dissimilar to our results, CAs in Chinese hamster lung fibroblast cell line and in CD‐1 mice mouse bone marrow cells (Ema et al, 2013; Naya et al, 2011), in Chinese hamster ovary cells (Kim et al, 2015), in human embryonic fibroblast cells (Nikitina et al, 2015), and in human bronchial epithelial and mesothelial cells (Lindberg et al, 2013) were not raised due to SWCNT exposure.…”

“…Dissimilar to our results, CAs in Chinese hamster lung fibroblast cell line and in CD-1 mice mouse bone marrow cells (Ema et al, 2013;Naya et al, 2011), in Chinese hamster ovary cells (Kim et al, 2015), in human embryonic fibroblast cells (Nikitina et al, 2015), and in human bronchial epithelial and mesothelial cells (Lindberg et al, 2013) were not raised due to SWCNT exposure.…”