Genotoxicity of wastewater samples from sewage and industrial effluent detected by the Allium root anaphase aberration and micronucleus assays

Grover, I. S.; Kaur, Satwinderjeet

doi:10.1016/s0027-5107(99)00065-2

Cited by 143 publications

(84 citation statements)

References 5 publications

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“…Higher plants such as A. cepa, V. faba and T. paludosa have large monocentric chromosomes in reduced numbers and are accepted as suitable test material for the study of environmental mutagenesis (Rank and Nielsen 1998;Grover and Kaur 1999;Patra and Sharma 2002;Sharma and Vig 2012). Allium cepa assay is one of the best and extensively used system for the determination of cytotoxic and genotoxic impacts of pollutants, due to its sensitivity (Barberio et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionmentioning

confidence: 99%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…The MNC value represented 2.74±1.56 of the positive control. The induction of micronuclei is usually the outcome of chromosome breaks/fragments or spindle poisoning which is an anomalous disjunction of chromosomes at anaphase stage of cell cycle (Dash et al 1988;Grover and Kaur 1999). Also, MNC are the results of acentric fragments or lagging chromosomes that fail to incorporate into either of the daughter nuclei during telophase in mitosis cell (Yi and Si 2007;Fernandes et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and Antimutagenic Effects ofCapparis spinosaL. on theAllium cepaL. Root Tip Meristem Cells

Sultan

Çelik

2009

Caryologia

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“…In situ studies have used the Trad/MCN test to detect genotoxicants in aquatic systems [Grant et al, 1992], river water [Duan et al, 1999;Jiang et al, 1999], and drinking water [Ma et al, 1985;Helma et al, 1994;Monarca et al, 1998], whereas the Vicia faba test has been employed in assays of river water [Rizzoni et al, 1995;Ji et al, 1999;Miao et al, 1999] and sediments [Rizzoni et al, 1995;Minissi and Lombi, 1997;Minissi et al, 1998]. The anaphase aberration test conducted in Allium cepa root tips has been used to study urban and industrial wastewater [Fiskesjo, 1985;Grover and Satwinderjeet, 1999;Monarca et al, 2000], drinking water [Fiskesjo, 1981], and fresh water [Smaka-Kincl et al, 1996].…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of drinking water disinfectants in plant bioassays

Monarca

Feretti

Zani

et al. 2005

Environ and Mol Mutagen

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The genotoxicity of two widely used drinking water disinfectants, sodium hypochlorite (NaClO) and chlorine dioxide (ClO2), and a new disinfectant, peracetic acid (PAA, CH3‐CO‐COOH), was evaluated in three short‐term plant tests: (1) induction of anaphase chromosome aberrations in the root cells of Allium cepa, (2) micronucleus induction in the root cells of Vicia faba, and (3) micronucleus induction in Tradescantia pollen cells. The study was carried out in the laboratory by directly exposing the plants to several concentrations of the disinfectants in redistilled water at unadjusted (acid) and adjusted (neutral) pHs. Both 0.1 and 0.2 mg/l NaClO induced chromosome aberrations in the Allium cepa test at acid pH, but concentrations up to 0.5 mg/l of all the disinfectants were negative at neutral pH. Concentrations ranging from 0.1 to 0.5 mg/l NaClO, ClO2, and PAA induced micronuclei in Vicia faba at acid pH, while 1–2 mg/l NaClO and ClO2 and 0.5–2 mg/l PAA gave positive responses at neutral pH. Most of concentrations of ClO2 produced positive responses in the Tradescantia micronucleus test. In general, the highest levels of genotoxicity were observed under acid conditions; at acid pH, significant effects were induced by low concentrations of ClO2 and PAA. Since the test concentrations of disinfectants are typical of those encountered in the biocidal treatment of tap water and similar concentrations are consumed daily by a large number of people, the genotoxicity of these compounds may constitute a significant public health concern. Environ. Mol. Mutagen., 2005. © 2005 Wiley‐Liss, Inc.

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Genotoxicity of wastewater samples from sewage and industrial effluent detected by the Allium root anaphase aberration and micronucleus assays

Cited by 143 publications

References 5 publications

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Genotoxic and Antimutagenic Effects ofCapparis spinosaL. on theAllium cepaL. Root Tip Meristem Cells

Genotoxicity of drinking water disinfectants in plant bioassays

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