Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay

Al-Amrah, Hadba; Aboznada, Osama Abdullah; Alam, Mohammad Zubair; ElAssouli, M‐Zaki; Mujallid, Mohammad I.; ElAssouli, Sufian M.

doi:10.3109/08958378.2014.970787

Cited by 21 publications

(8 citation statements)

References 39 publications

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“…The tail moment in buccal cells of smokers was found to be 186 ± 26, which is 371.9% higher than the tail moment in buccal cells of non-smokers. The other comet parameters such as tail length, % tail DNA, and fragmented DNA were 456 ± 71, 97.0 ± 19, and 32.0 ± 3.3, respectively, in buccal cells of smokers, whereas in control group (non-smokers), the values of tail length, % tail DNA, and fragmented DNA were extremely low [72].…”

Section: Life Style Dietary Genetic and Healthy Factorsmentioning

confidence: 87%

“…The comet assay in buccal cells has been used to evaluate DNA damage induced by different materials such as mouthrinses [50], metals released from orthodontic appliances [51][52][53][54][55][56][57][58][59], ionizing radiation [60], as well as assessment of DNA damage, and its modulation by life-style, dietary, genetic and healthy factors [61][62][63][64][65][66][67][68][69][70][71][72][73][74], occupational exposure [66][67][68][69][75][76][77][78][79][80][81][82], and environmental exposure [83][84][85][86]. Different procedures have been used in collecting and processing the samples that are presented and discussed in Rojas et al [33].…”

Section: Use Of Comet Assay In Buccal Cellsmentioning

confidence: 99%

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Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Sánchez-Alarcón¹,

Milić²,

Gómez‐Arroyo³

et al. 2016

Environmental Health Risk - Hazardous Factors to Living Species

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DN" damage risk assessment in comet assay by the use of buccal mucosa cells has great advantages in comparison with other cell type sample due to more safely, easier, cheaper, and non-invasive method for in vivo studies. "ccording to the OECD Guidelines, the in vivo mammalian alkaline comet assay is well-established and validated method for measuring DN" strand breaks in single eukaryotic cells. Considering exposure to xenobiotics and endogenous damage inductors, buccal mucosa cells are the first to be in direct contact after exposure and this makes them an ideal biomatrices in evaluation of the level of individual genotoxicity to several compounds already mentioned. Their clinical diagnostic applicability confers a potential use in patients across time. However, the number of publications referring to the human buccal comet assay is low in the last two decades. This low growing interest may be explained by several factors, including its relative technical problems. Different procedures have been used in collecting and processing the samples. In order to have widespread acceptance and credibility in human population studies, the comet assay in buccal cells requires standardization of the protocol, of parameters analyzed, and a better knowledge of critical features affecting the assay outcomes, including the definition of the values of spontaneous DN" damage. There is a need for further collaborative work as in the HUMN micronucleus assay on lymphocytes and HUMNxL micronucleus assay on buccal cells collaborative projects. The creation of a network of laboratories will allow more focused validation studies, including the design of a classic, historic, prospective cohort study in order to explore the link between measures of genetic instability in the buccal mucosa and the risk of cancer and other chronic-degenerative diseases. One such network connection will start in as a COST project under the name hCOMET The comet assay as a human biomonitoring tool launched by Prof. "ndrew Collins.

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Section: Life Style Dietary Genetic and Healthy Factorsmentioning

confidence: 87%

Section: Use Of Comet Assay In Buccal Cellsmentioning

confidence: 99%

Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Sánchez-Alarcón¹,

Milić²,

Gómez‐Arroyo³

et al. 2016

Environmental Health Risk - Hazardous Factors to Living Species

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“…The popularity of e-cigarettes is attributable to the perception that these cigarettes are less harmful than traditional cigarettes, their easy availability, cheaper price, the spread of vaping as a trend, and the variety of flavors available (de Andrade et al, 2015;Tomashefski, 2016;Collins et al, 2019). However, several studies have reported that, like conventional cigarettes, waterpipe smoking also has genotoxic effects (Al-Amrah et al, 2014;Katarkar et al, 2014;DehghanNezhad et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic profile among e-cigarette consumers, cigarette smokers, and nonsmokers

Al-Qudaihi

Al-Rajoudi

ALJERAYED

et al. 2023

EMCR

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It is widely believed that e-cigarettes are less harmful than conventional cigarettes because they have a lower nicotine content. In contrast to this notion, several in vitro studies have evaluated and demonstrated the genotoxicity associated with e-cigarette smoking. However, there is a lack of human studies on the genotoxicity of e-cigarettes. This pilot study evaluated and compared indicators of genotoxicity in e-cigarette users, cigarette smokers, and nonsmokers. A total of 84 healthy participants, including 20 e-cigarette users, 31 cigarette smokers, and 33 nonsmokers, were recruited. Genotoxicity was evaluated by measuring tail moment (TM), tail length (TL), and % tail DNA intensity (%T) using the comet assay as an indicator of DNA damage in blood and detecting micronuclei in buccal cells with the buccal micronucleus (MN) cytome assay. Bivariate analyses showed that there was no significant difference in TM and TL between e-cigarette users and cigarette smokers, but in both groups, the three parameters were significantly higher than that in nonsmokers (p < 0.02). In contrast, the frequency of micronuclei in e-cigarette users (40%) was higher than that in cigarette smokers (27.5%). Our findings indicate that e-cigarettes have a similar genotoxic effect to regular cigarettes and, therefore, contradict the notion that e-cigarettes are safer than regular cigarettes.

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“…Five studies ( 16 – 20 ) applied standardized questionnaires, however, without citing any reference. Six others ( 9 , 11 – 14 , 21 ) applied non-standardized questionnaires and three ( 8 , 10 , 15 ) did not mention how patients’ information was selected. It is interesting to note that there is a pressing need to standardize items in epidemiological questionnaires used in studies addressing the narghile use ( 28 ).…”

mentioning

confidence: 99%

“…In front of the confusion about how to quantify narghile use, a specific international codification is immediately needed ( 6 ). 2) Information about the type of used tobacco was specified only in three studies ( 8 , 13 , 15 ). The lack of information about the different types of used narghile tobacco makes comparison difficult, because in the case of tombak or jurak , in comparison to tabamel , the pattern is different ( 6 ).…”

mentioning

confidence: 99%

Critical analysis of the published literature about the effects of narghile use on oral health

Khemiss

Rouatbi

Berrezouga

et al. 2015

Libyan Journal of Medicine

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Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay

Cited by 21 publications

References 39 publications

Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Genotoxic profile among e-cigarette consumers, cigarette smokers, and nonsmokers

Critical analysis of the published literature about the effects of narghile use on oral health

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